Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"SNARE protein"”.
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Biochemical Analysis of SNARE Protein Interactions – Role of Transmembrane Domain
SNARE (soluble NSF attachment protein receptor) Proteine umfassen verschiedene Familien von Proteinen, welche in Eukaryonten von der Hefe bis hin zum Menschen vorkommen. Sie sind für die Membranfusion bei zellulären Prozessen, wie Vesikeltransport, Neurotransmitterausschüttung oder …
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An evolutionarily conserved regulatory mechanism for endosomal membrane trafficking
… cells is facilitated by the formation of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes; a process that is regulated by Sec1p/Munc18 (SM) proteins. Membrane fusion has been conserved through evolution and hence a lot or our knowledge about the …
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Re-localization of SNARE proteins in mouse sperm prior to the acrosome reaction
The principal role of SNARE proteins is to arbitrate vesicle fusion to a target membrane. Formation of tripartite SNARE protein complexes between SNARE proteins on opposing membranes is the minimal requirement for membrane fusion. The SNARE protein family is large, consisting of more than 60 …
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Vps1's Role From the Early Endosome to the Golgi
Vps1 is a dynamin-like protein that has been implicated in various cellular events, including protein trafficking from the late Golgi-to-endosome, peroxisome fission, vacuole fission and fusion, and retention of Golgi proteins. We recently observed in FM4-64 pulse-chase experiment with vps1 null …
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SNARE specificity in membrane protein traffic
Recent findings indicate that there is a role for SNAREs in ion channel regulation. With the discovery of the vesicle trafficking SNARE protein NtSYP121 came the development of the predicted cytosolic domain fragment of the protein. This Sp2 fragment was found in studies to block the responses of …
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Mutational analysis of the Vam3 transmembrane domain and its effects on vacuole fusion in Saccharomyces cerevisiae
Vam3 is a SNARE protein essential in yeast vacuole homotypic fusion. The specific role of the Vam3 trans-membrane domain (TMD) during vacuole fusion remains unclear. Previous work had shown that replacing Vam3 TMD with a prenyl lipid anchor (CCIIM) results in attenuated fusion and accumulation of …
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Charakterisierung von SNARE-Proteinen in der Hefe Saccharomyces cerevisiae
SNARE-Proteine sind in allen Eukaryonten an der Fusion von Transport-Vesikeln mit Zielmembranen beteiligt, wobei sie Komplexe bilden in denen jeweils 3 Q-SNAREs mit einem R-SNARE interagieren. Im ersten Teil der Dissertation wurde über einen Multicopy-Suppressor-Screen versucht, zusätzliche …
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Vesicle-associated Membrane Proteins in Airway Mucin Secretion
… by the collaborative interaction between a SNARE complex and an SM (Sec1/Munc18) protein. One type of SNARE protein, a Vesicle-Associated Membrane Protein (VAMP), has been shown to regulate exocytosis in multiple cell types.</p> <p>Here, we report that VAMP8 has the highest gene expression …
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Untersuchung von Septin-Interaktionen
… eine neue Klasse stark konservierter homologer Proteine, die 1971 in der Hefe Saccharomyces cerevisiae entdeckt wurden. Sie gehören zur Gruppe der GTPasen und sind an Zytokinesevorgängen wesentlich beteiligt. Doch auch in postmitotischen Zellen werden Septine exprimiert, so etwa im Gehirn- und …
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Functional Analysis of AtVAMP714 gene in Arabidopsis
… tag was positioned between ATP-binding protein and vesicle associated membrane protein genes. Cloned mutant locus and expression studies indicated that the vesicle associated membrane protein gene is upregulated in the activation tagging line. The aim of this work is to investigate the …
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Dynamics and Turnover of Lipid and Protein Domains in Cells and Supported Bilayers
<p>Protein and lipid clustering is an important mechanism for cell processes such as exo- and endo-cytosis and creating functional signaling complexes. It has been seen that both lipids and proteins are involved with the formation of cluster domains on cell membranes, yet little is known about the …
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Lipid Rafts in Pancreatic Beta- and Alpha-cell Stimulus-secretion Coupling
… lipid rafts. Numerous signaling and transport proteins have been found to be targeted to lipid raft microdomains, where the function of the associated membrane proteins could be distinctly regulated. I have identified the expression of lipid raft constituent proteins, caveolin-1/2 in pancreatic …
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Mechanistic Insights into the Role of Munc13 in Synaptic Vesicle Docking, Priming, and Fusion
… influx of Ca2+, which is sensed by the complex protein machinery at the axon terminal. In order for these Ca2+-mediated fusion events to occur in the correct time and place, protein machines interact with neurotransmitter filled vesicles to dock and prime them for release. Munc13 is one of the …
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Characterization Of Protein Prenyltransferases And Protein Prenylation In Plasmodium Falciparum
… urgent to identify new drug targets. Many proteins involved in eukaryotic signal transduction and cell cycle progression undergo post-translational lipid modification by a prenyl group. Protein prenyltransferases, which catalyze the post-translational prenyl modification, have been …
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Investigating the Role of Drosophila Tomosyn in Synaptic Strength and Plasticity
… by precise spatial and temporal regulation of SNARE assembly that occurs at specialized presynaptic subdomains in response to transient calcium influx following an action potential. SV fusion from the presynaptic terminal results in a postsynaptic response that varies in size depending on …
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Novel Insights into the Regulation and Function of SGK3
… substrate unique to SGK3, the endosomal SNARE protein, Syntaxin 12 that is not phosphorylated by Akt. This finding represents the first, non-Akt related function of SGK3 at the endosomes and further explains why SGK3 requires regulation by two distinct classes of PI3K.
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Biophysikalische und thermodynamische Charakterisierung der neuronalen SNARE-Komplexbildung
… werden durch eine konservierte Proteinfamilie, die so genannten SNARE-Proteine, vermittelt. SNARE-Proteine bilden einen stabilen α-helikalen Komplex zwischen zwei Membranen aus. Dabei bilden die plasmamembranständigen Proteine SNAP-25 und Syntaxin einen Akzeptorkomplex für das in …
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Lipid composition and membrane physical parameters regulate priming and SNARE function in yeast vacuole membrane fusion
… Apart from a family of highly conserved proteins called SNAREs, membrane fusion also requires a set of regulatory lipids that includes ergosterol, diacylglycerol, phosphatidic acid and phosphoinositides. Although a lot is known about the SNARE protein machinery, the role of lipids and the …
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Der Syntaxin 1-Cluster - Organisation und Dynamik einer supramolekularen Struktur der Plasmamembran
… Fluid-Mosaic-Modells erwarten würde. Viele Proteine sind nicht uniform verteilt, sondern in sogenannten Membran-Domänen angereichert. Die Frage, wie die Heterogenität der Plasmamembran zustande kommt und über die Zeit erhalten bleibt, ist nicht abschließend geklärt und die diesbezüglich …
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INVESTIGATING THE ROLE OF SNAP29 AND THE AUTOPHAGY PATHWAY IN DROSOPHILA MELANOGASTER MODELS OF C9ORF72 - LINKED ALS/FTD.
… pathway, responsible for clearing damaged proteins and organelles, progressively declines in normal aging and in neurodegenerative diseases, leading to toxic protein aggregate accumulation. Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), two devastating …
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