Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 130 for “"SNARE"”.
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Structural Studies of Complexin/SNARE Interactions
… to delineate interactions between complexin and SNARE components that lead to the formation of a primed minimal fusion machinery. We have generated and used new constructs of short forms of synaptobrevin and complexin, as well as constructs of SNAP-25 and syntaxin that have previously been shown …
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SNARE specificity in membrane protein traffic
Recent findings indicate that there is a role for SNAREs in ion channel regulation. With the discovery of the vesicle trafficking SNARE protein NtSYP121 came the development of the predicted cytosolic domain fragment of the protein. This Sp2 fragment was found in studies to block the responses of …
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Eine funktionelle Charakterisierung der frühendosomalen SNARE-Proteine
… von Vesikeln, wobei dieser letzte Schritt von SNARE-Proteinen bewerkstelligt wird. Neben deren Funktion in der Fusion wird schon seit einiger Zeit auch über eine Beteiligung an dem, der Fusion vorausgehenden Prozess des Docking, d.h. der stabilen Verknüpfung der Organellen spekuliert. Diese …
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Biophysikalische und thermodynamische Charakterisierung der neuronalen SNARE-Komplexbildung
… konservierte Proteinfamilie, die so genannten SNARE-Proteine, vermittelt. SNARE-Proteine bilden einen stabilen α-helikalen Komplex zwischen zwei Membranen aus. Dabei bilden die plasmamembranständigen Proteine SNAP-25 und Syntaxin einen Akzeptorkomplex für das in der Vesikelmembran sitzende …
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Structural and Functional Studies of Munc18 and SNARE Proteins
… are proteins from the Sec1/Munc18 (SM) and SNARE (soluble N-ethylmaleimide sensitive factor attachment protein receptors) families. SNAREs function by forming a four-helix bundle called SNARE complex; assembly of the SNARE complex brings two membranes together and is key for membrane fusion. …
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Unraveling the Role of SNARE Interactions in Neurotransmitter Release
… 1 and SNAP-25, which are collectively known as SNAREs and form a tight complex (the core complex). The assembly of the core complex may mediate membrane fusion. Complexin is a highly conserved cytoplasmic protein that binds tightly to the SNARE complex. Analysis of the interaction between …
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Charakterisierung von SNARE-Proteinen in der Hefe Saccharomyces cerevisiae
SNARE-Proteine sind in allen Eukaryonten an der Fusion von Transport-Vesikeln mit Zielmembranen beteiligt, wobei sie Komplexe bilden in denen jeweils 3 Q-SNAREs mit einem R-SNARE interagieren. Im ersten Teil der Dissertation wurde über einen Multicopy-Suppressor-Screen versucht, zusätzliche …
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SNARE-mediated exocytosis of MUC2 from intestinal goblet cells
… Herein we describe for the first time that the SNARE complex in goblet cells mediates mucin exocytosis and degradation of these regulatory pathways exacerbates pathogenesis of the intestinal pathogen Entamoeba histolytica. The SNARE complex is the classical exocytosis machinery as the effector …
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Munc18-1 Is a Master Orchestrator of Synaptic SNARE Assembly
Pages 114-185 are misnumbered as pages 115-186.
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Biochemical Analysis of SNARE Protein Interactions – Role of Transmembrane Domain
SNARE (soluble NSF attachment protein receptor) Proteine umfassen verschiedene Familien von Proteinen, welche in Eukaryonten von der Hefe bis hin zum Menschen vorkommen. Sie sind für die Membranfusion bei zellulären Prozessen, wie Vesikeltransport, Neurotransmitterausschüttung oder …
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Regulation of SNARE-dependent fusion in an in vitro system
… Central to this machinery are members of the SNARE (soluble NSF attachment protein receptor) family of proteins. The process of SNARE-mediated membrane fusion needs to be tightly regulated and the SNARE proteins are partially responsible for the specificity in communication between eukaryotic …
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Regulation of SNARE-Mediated Synaptic Vesicle Release by Synaptotagmins and Complexins
… influx through voltage-gated Ca2+ channels. The SNARE (Soluble N-ethylmaleimide-sensitive-factor Attachment Protein Receptors) complex is the core component of the fusion machinery in the presynaptic terminal, as it forms a physical bridge between the vesicular membrane and the presynaptic target …
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Analysis of Synaptotagmin-SNARE Complex Interactions by One-Dimensional NMR Spectroscopy
… numerous proteins at the neuronal synapse. The SNARE proteins form a complex that mediates fusion between the synaptic vesicle and plasma membrane. The protein synaptotagmin-1 is the major sensor for the calcium concentration. Synaptotagmin-1 and the SNARE complex are therefore believed to …
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Biophysical Characterization of SNARE Complex Disassembly Catalyzed by NSF and alphaSNAP
… sind Vertreter der konservierten Familie der SNARE-Proteine von essentieller Bedeutung. Man vermutet, dass gerichtete Komplexbildung zwischen SNAREs auf gegenueberliegenden Membranen dazu fuehrt, dass die zu fusionierenden Mebranen in raeumliche Naehe gezogen werden und schliesslich …
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Regulation of SNARE function during sperm capacitation to promote the acrosome reaction
… factor attachment protein receptor (SNARE) complex formation and movement into membrane rafts in preparation for the acrosome reaction. Two t-SNAREs and a v-SNARE form a trans-SNARE complex, which is required for exocytosis. The core complex along with other regulatory proteins exerts …
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Single-molecule fluorescence resonance energy transfer study of SNARE-mediated membrane fusion
… factor attachment protein receptor (SNARE) play a central role in fusion processes which is also regulated by many accessory proteins, such as synaptotagmin, complexin and Munc18. By a new lipid mixing method at the single-vesicle level, we are able to accurately detect different …
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Molecular mechanisms of snare assembly and expulsion of intravesicular contents in cell secretion
… factor (NSF)-attachment protein receptors called SNAREs, have been implicated in membrane fusion in cells. For example in neurons, target membrane proteins SNAP-25 and syntaxin (t-SNARE) present at the porosome base, and a synaptic vesicle-associated membrane protein (v-SNARE), are part of the …
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Re-localization of SNARE proteins in mouse sperm prior to the acrosome reaction
The principal role of SNARE proteins is to arbitrate vesicle fusion to a target membrane. Formation of tripartite SNARE protein complexes between SNARE proteins on opposing membranes is the minimal requirement for membrane fusion. The SNARE protein family is large, consisting of more than 60 …
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Neue Einblicke in die SNARE-vermittelte Fusion: Detektion einzelner Proteoliposomen mit einem konfokalen Mikroskop
… sie durch die konservierte Proteinfamilie der SNAREs angetrieben werden. Diese Proteinen haben eine etwa 60 Aminosäuren lange, in Heptad-Wiederholungen angeordnete Sequenz, das SNARE-Motiv. Jeweils vier verschiedene SNARE-Motive sind auf die fusionierenden Membra-nen verteilt. Bei der Fusion …
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