Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"SNAP-tag"”.
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Photoimmunotheranostic targeting of CSPG4-positive melanoma cells using SNAP-tag technology
… disease if the cancer is detected in the early stages. Once melanoma enters the later stages, and particularly in the metastatic phase, recurrence is probable, and no adequate treatment exists. Previous work in this group has shown that photodynamic therapy (PDT) presents an opportunity to induce …
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Development of SNAP-tag-based fusion proteins targeting HIV-1 viral reservoirs
… a J3-ETA IT, this work sought to produce a J3-SNAP fusion protein by osmotic stress expression in the presence of compatible solutes in the periplasmic space of E. coli. SNAP-tag is a self-labelling protein that covalently binds benzylguanine (BG)-modified substrates in a 1:1 stoichiometric …
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Establishment of recombinant antibody technologies allowing for the generation of SNAP-tag fusion proteins
… conjugates, which may exhibit multiple advantages over their unconjugated counterparts, but their general use in clinical application has been restricted due to developmental deliberations. Historical conjugation strategies used for the generation of ADCs commonly resulted in heterogeneous …
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Validation of an aASPH-SNAP-tag fusion protein for immunodiagnosis of triple-negative breast cancer
… fused to its light chain variable region. The SNAP-tag is commonly known as the engineered version of the human DNA repair enzyme O6 -alkylguanineDNA-alkyltransferase (AGT) that has the ability to recognize and bind to any benzyl-guanine modified substrates in a 1:1 stoichiometry. This makes …
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Generation of recombinant SNAP-tag based antibody fusion proteins for immunotherapy of acute myeloid leukemia (AML)
… fused to a self labelling human protein called SNAP-tag were generated. SNAP-tag was used for site-specific conjugation to O6 -benzylguanine (BG) derivatives to demonstrate functionality against overexpressed and therapeutically relevant AML antigens CD33 and CD45. The scFv component of each …
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Developing novel SNAP tag-based antibody fusion proteins directionally conjugated to fluorophores for immunophenotyping of acute myeloid leukemia
… maturation arrest at a particular developmental stage, which is specific to each subtype of leukemia. The treatment strategies for acute myeloid leukemia include chemotherapy, bone marrow transplantation, radiotherapy, and immunotherapy, with current research focusing on antibody-based …
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Comparison of the therapeutic efficacy of SNAP-tag fusion protein and the synergistic actions of chemotherapy and photodynamic therapy in killing resistant melanoma
… therapy, using the self-labelling activity of SNAP-tag fusion protein to conjugate synthetic small molecule lead substance toxin such as monomethyl Auristatin F (MMAF or AURIF). This cytotoxic payload was delivered to targeted cells, through genetic fusion of SNAP-tag to three different single …
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Development of SNAP-tag based fusion proteins as novel auristatin F-containing immunoconjugates and photoimmunotheranostics in the detection and treatment of triple-negative breast cancer
… conjugates (ADCs), might exhibit several advantages compared to their small-molecule counterparts, but their widespread clinical use is hampered by various developmental considerations. Traditional conjugation strategies employed to arm mAbs with cytotoxic warheads, usually give rise to …
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Evaluation of tumour-associated antigens to optically label cutaneous basal cell carcinoma for surgical excision
… and design, engineer, and test a range of SNAPtag–based antibody fusion proteins that would specifically bind to and detect such BCC cell surface receptors. The SNAP-tag antibody technology is based on the genetic fusion of a disease-specific ligand to a protein tag derived from …
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Generation of Glioblastoma specific SNAP based antibody fusion proteins for future radiolabelling application
… tissue of the GBM tumour microenvironment. SNAP-tag® fusion antibodies against the abovementioned TAAs were employed in this study. The fusion antibody is a recombinant antibody-based fusion protein created by fusing the SNAPtag®, a modified version of the DNA repair enzyme O6 -alkylguanine …
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Synthesis and biological evaluation of auristatin-F recombinant antibody-drug conjugates for cancer therapy
… potential and specificity towards cancer cells. SNAP-tag site-directed conjugation was employed in order to overcome the current challenges of heterogeneous ADC combination products resulting from standard conjugation methods. The SNAP-tag fusion proteins were generated using recombinant DNA …
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Antibody engineering to evaluate binding, internalisation, and intracellular routing of tumour-targeting fusion proteins
… antibody derivative was genetically fused to a SNAP-tag, enabling stable and site-specific conjugation of the scFv to diagnostic and therapeutic substrates. To enhance antigen binding, internalisation, and therapeutic efficacy, a bivalent scFv fusion protein was created in tandem with the …
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Development of potential immunodiagnostic & therapeutic techniques using SNAP-fusion proteins as tools for the validation of Triple-negative Breast Cancer
… (HER2). TNBC is commonly associated with early stage metastasis with low survival rates as well as a high frequency of recurrence and proves to be problematic in both the young and elderly female populations. Conventional diagnostic methods for TNBCs include mammography, magnetic resonance …
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Chemi-Optogenetics: Photoactivatable Probes To Control Protein Proximity And Cellular Processes
… of self-labelling proteins (i.e. Halo-tag, SNAP-tag, eDHFR), to develop a series of tools that localize proteins of interest to its target with spatiotemporal precision. The local proximity of a protein and its substrate is sufficient to initiate a desired biological effect, as the …
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Yeast-based vaccine approaches to cancer immunotherapy
… method is site-specific (based on the SNAP-tag) and designed to facilitate antigen release in the dendritic cell phagosome and subsequent translocation for cross-presentation.
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QUANTIFYING OLIGOMERIC STATES OF PROTEINS IN-CELL VIA SINGLE-MOLECULE SUPER-RESOLUTION MICROSCOPY
… the dimerization of SOD1 by imaging SOD1-Halo-tag proteins conjugated with FRET donor fluorophore, PA-JF549, and acceptor fluorophore, JF646, in COS7 cells. Analyzing the FRET contribution on donor's intensity distributions allows us to estimate the dimerization of SOD1 proteins in cells. To …
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Single Molecule Ligand Binding Studies on CCR5 by Fluorescence Cross-Correlation Spectroscopy
… We fluorescently labeled CCR5 using the SNAP tag, a functional tag derived from DNA alkyl transferase, with Alexa-488. We show similar cell surface expression for CCR5-SNAP and wild-type CCR5 using flow cytometry with different epitope recognizing antibodies. We demonstrate that CCR5-SNAP …
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Establishment of enhanced protein purification strategies for recombinant immunotherapeutics
… the proteins of interest, such as the affinity tag incorporated in the recombinant antibody, and its Isoelectric value (pI), largely contribute to the choice of purification technique as demonstrated by the well characterized mammalian-expressed SNAP tag-based antibody fusion protein …
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Generation and therapeutic evaluation of recombinant immunotherapeutics on CD64+ monocytic cell lines and polarized ex vivo differentiated human macrophages
… aeruginosa exotoxin A (ETA`), or 2) a SNAP-tag fusion protein (FP) to allow chemical coupling of the antibody fragment to the cytostatic drug monomethyl auristatin F (AURIF) or the light sensitive dye IR700. By so doing generating an antibody drug conjugate (ADC) or a …