Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 40 for “"SMAD2"”.
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SMAD2/3 - FOXH1 regulation during cell cycle progression upon differentiation
… of TGFβ signalling via its downstream effector SMAD2/3 is directed by cell cycle regulators and especially cyclin Ds. Indeed, cyclin Ds direct cell fate propensity either by activating CDK4/6 activity, which results in blocking SMAD2/3 nuclear entry or by directly binding to developmental genes …
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Role of SMAD2 and SMAD3 on Adipose Tissue Development and Function
… development, function, and maintenance.</p> <p>SMAD2/3 proteins are downstream mediators of transforming growth factor-β (TGFβ) family signaling, including activins, which regulate critical preadipocyte and mature adipocyte functions. Previous studies have demonstrated that Smad2 global knockout …
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Semi-Synthesis of the Transcription Factor SMAD2 Containing Caging Groups and Phosphoaminoacid Analogues
… to the study of the cellular signaling protein Smad2. Smad2 is activated by phosphorylation, which is the best characterized reversible PTM. Once phosphorylated, Smad2 accumulates in the nucleus of cells where it helps to direct transcriptional changes that affect cell behavior. Techniques were …
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Growth Differentiation Factor 11 Promotes Survival of Mouse Retinal Ganglion Cells in Vitro through Smad2/3 Activation
… RGC survival in vitro through activation of pSmad2/3.
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Activin/Nodal Signalling Controls the Epigenome and Epitranscriptome of Human Pluripotent Stem Cells
… mechanisms controlled by the Activin/Nodal-SMAD2/3 signalling pathway, whose activity dictates the balance between hPSC pluripotency and differentiation. First, I show that SMAD2/3 modulates the chromatin epigenetic landscape of hPSCs by cooperating with the pluripotency factor NANOG to …
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SMAD2, SMAD3 et SARA : des facteurs clés de la signalisation du TGFβ impliqués dans l’invasion des cellules cancéreuses stimulée par le microenvironnement hypoxique
… acteurs principaux de la signalisation du TGF SMAD2 et SMAD3, jouent des rôles opposés dans l’invasion des cellules cancéreuses : SMAD2 agissant comme inhibiteur, et SMAD3 promouvant la production d’invadopodes. Ces rôles opposés peuvent être attribués à la modulation de gènes cibles distincts …
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Oncogene-Induced Signaling Heterogeneity in Lung Cancer
… readouts - phospho-Erk1/2, phospho-Stat3, Smad2/3, β-catenin, P65, and Foxo1 and quantitatively analyze thousands of cells with defined set of genetic changes. I ask - Can I utilize oncogene-induced signaling alterations in single cells to identify novel targetable vulnerabilities? Using …
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Signal transduction of transforming growth factor-Beta in cytotoxic T cells
… TGF-b resultierte in der Unfähigkeit der Zellen, Smad2 ligandeninduziert zu phosphorylieren. Entweder wurde überhaupt keine Phosphorylierung beobachtet, oder eine anhaltende Phosphorylierung von Smad2 unabhängig vom Vorhandensein des Liganden. Des weiteren stellten wir einen Zusammenhang zwischen …
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Verwendung von Gene-Targeting-Techniken zur Etablierung neuer Mauslinien mit Mutationen in B-Zell-Signalwegen
… war es, die Rolle des TGF-β Signalmediators SMAD2 auf B-Zellebene näher zu untersuchen. Von Erwin Böttinger bekamen wir eine Mauslinie, in der das Smad2-Gen gefloxt ist, die mit der CD19-Cre Maus gekreuzt wurde. So wurde eine B-Zell spezifische SMAD2 knock out Maus (bSmad2-/-) erzeugt. Meine …
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Regulation der Differenzierung von Ratten-Calvaria-Osteoblasten unter Einfluss von Wachstumsfaktoren
… von Erk1 und Erk2, sowie von Smad1 und Smad2 mit Hilfe eines Kinase-Aktivitätsassays sowie der Westernblot-Analyse untersucht werden. Im Rahmen dieser Arbeit soll weiterhin untersucht werden, welche Bedeutung die Wachstumsfaktoren TGF-b1 und BMP-4 auf die Aktivität der alkalischen …
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Self-Renewal Requirements of Human Embryonic Stem Cells and Their Engraftment Potential in Mouse Blastocysts
… is activated (through the signal transducer Smad2/3) while the BMP/GDF branch (Smadl/5) is only active in isolated mitotic cells. Upon early differentiation, Smad2/3 signaling is decreased while Smadl/5 signaling is activated. We next tested the functional role of TGFp/activin/nodal signaling …
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The regulation of TGFβ/BMP signalling by deubiquitylating enzymes
… OTUB1 was recruited to the active phospho-SMAD2/3 complex only upon TGFß induction and OTUB1 had a crucial role in TGFß-mediated gene transcription and cellular migration. OTUB1 inhibited the ubiquitylation of phospho-SMAD2/3 by binding to and inhibiting the E2 ubiquitin-conjugating enzymes …
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Effect of Anti-fibrotic Drug (Pirfenidone) on TGF-β Signaling Pathway in Ocular Fibrotic Disorders
… by preventing nuclear accumulation of active Smad2/3 complexes rather than phosphorylation of Smad2/3. Conclusions: These results collectively provide a rational background for future evaluation of pirfenidone as a potential anti-fibrotic agent for treating proliferative vitreoretinopathy and …
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Modulation of human adipose-derived stem cells and dermal fibroblasts in co-cultures by activation of the IL-6 trans-signalling pathway via the soluble IL-6Rα receptor
… and VEGF) and biomarker expression (TGF β1RII, SMAD2/3, SMAD 1/5/9 and SMA) were quantitated. Although both cell types secreted IL-6, ADSC basal levels were 100-fold that of DF. Both cell types expressed CD130 (gp130) signalling transductor but were negative for the membrane-bound CD126 receptor …
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14-3-3 Zeta Overexpression Serves As A Novel Molecular Switch Turning Tgf-Beta From Tumor Suppressor to Tumor Promoter
… is an important co-transcriptional factor for p-smad2 to activate PTHrP expression and bone osteolytic effect. Taken together, we reveal a novel mechanism that 14-3-3ζ dictates the tumor suppressor or metastases promoter activities of TGF-β signaling pathway through switching p-smad2 binding …
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Transcriptome Analysis of Sea Lamprey Embryogenesis
… functional roles. Genes TGFBR1 (protein kinase), SMAD2 (signal transducer), TRAF6 (signal transducer), PCNA (DNA replication), SLX4 (endonuclease), and PXN (focal adhesion) were selected for their NGS expression levels, GO annotations, and biological function significance. Their NGS expression …
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Characterisation of BMP Processing and Signalling in Adipocytes
… that BMP8B signals through both SMAD1/5/8 and SMAD2/3 pathways in several cell types, but the impact of such unusual signalling specificity in adipocyte function is unknown. Thus, investigating BMP8B functions is crucial, both for therapeutic and molecular biology insights. My PhD project aims …
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Molecular Mechanisms Underlying The Transcriptional Regulation of T Helper 17 and Regulatory T Cells
… downstream of TGF-b, we found that while Smad2, Smad3 and Smad4 are required for the generation of iTreg cells, only Smad2 is indispensable for the induction of IL-17-producing cells, suggesting that TGF-b induces these T helper lineages through differential signaling pathways. Thus, our …
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Smad Proteins and theTgf-Beta Signaling Pathway During In Vitro Progression of Hpv16-Immortalized Human Keratinocytes' and 'Microarray Gene Expression Profiling of Exfoliated Cervical Cells'
… of Smad3 and Smad4, and phosphorylation of Smad2 are affected during in vitro progression. We did not find any consistent decrease in protein expression of Smad2, Smad3 or Smad4; or increase in protein expression of inhibitory Smad7 that paralleled the increasing loss of sensitivity to the …
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