Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"SMAC"”.
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SMAC Mimetics Sensitize HIV-Infected Cells to MG1-Mediated Death
… mitochondria derived activator of caspases (SMAC) mimetic (AEG 40730, LCL-161, birinapant) to kill the latently HIV-infected lymphocytic cell line J1.1 and myelocytic cell line OM10.1. In HIV-infected cell lines, it was shown that the order of administration of MG1 and SMAC mimetics is …
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Smac Mimetic and Combination Immunotherapy for the Treatment of Urothelial Cell Carcinoma
… proteins regulate cell death signaling cascades. Smac mimetic (SM) compounds are antagonists of these proteins and are being evaluated for their anti-cancer efficacy and immunomodulatory effects. Here, I adopted an orthotopic murine model of urothelial carcinoma (MB49) resistant to prominent …
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Characterization of a Small Molecule Smac Mimetic's Role in Inducing Apoptosis in Human Cancer Cells
… mitochondria-derived activator of caspases (Smac), a mitochondria-released protein during apoptosis, binds to IAPs BIR domains with four amino acid residues (AVPI) and releases the inhibition caused on caspases by IAPs. With the idea of designing a Smac mimicking drug, that will induce …
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Analysis of a novel cytotoxic drug induced TRAIL-R2 signalling complex using a new FACS-based Smac/DIABLO release assay
… of proapoptotic factors such as cytochrome c and Smac/DIABLO into the cytosol. As the release of Smac/DIABLO is important in apoptosis and possibly impaired in DR5-silenced cells, we developed a FACS based Smac/DIABLO release assay in 5-Flurouracil (5-FU) stimulated HCT116 cancer cells. Using this …
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Roles of protein kinase C in cell death and breast cancer
… several breast cancer cells. Here we show that Smac, a proapoptotic protein, associates with PKCδ in many different cancer cell lines. Furthermore, the PKCδ-Smac association was dissociated upon paclitaxel treatment. Upon PKC activation with TPA the PKCδ-Smac complex was stabilized and the …
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Overcoming Acquired Resistance to BRAF Inhibitors by Novel Synergistic Drug Combination and Discovery of Novel Smac Mimetics as Selective Survivin Inhibitors
… mitochondria-derived activator of caspases (Smac) is a rational strategy to design potential IAP inhibitors. In this report, we used the bioactive conformation of AVPI tetrapeptide in the N-terminus of Smac as a template and performed a shape-based virtual screening against a drug-like …
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Calpain 9 Functions in TNF Receptor Mediated Apoptosis
… Mitochondria-Derived Activator of Caspases, Smac, from the mitochondria relieves IAP Caspase inhibition, activating apoptosis. Our lab has developed a small molecule Smac mimetic. Surprisingly, approximately 25% of cell lines show single agent Smac mimetic sensitivity through activation of …
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Loss of Caspase-8 Function In Combination With Smac Mimetic Treatment Sensitizes Head and Neck Squamous Carcinoma to Radiation Through Induction of Necroptosis.
… mitochondria-derived activator of caspase (SMAC) mimetic, Birinapant, when combined with pan-caspase inhibitors zVAD-FMK or emricasan. Strikingly, inhibition of CASP8 function via knockdown or emricasan treatment was associated with enhanced radiation killing by Birinapant through induction …
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Protein Kinase C as an Apoptosis Regulator and a Potential Prognostic Factor in Breast Cancer
… of apoptosis regulation since it interacts with Smac in breast cancer cells. The interaction is mediated via the C1b domain in the regulatory domain of PKCδ and depends on the N-terminus of Smac. Treatment with lethal triggers, such as paclitaxel, causes dissociation of the interaction, which is …
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Induction of Synthetic Lethality In Mutant Kras Cells For Non-Small Cell Lung Cancers Chemoprevention and Therapy
… mitochondria-derived activator of caspases (Smac) mimetic. So the combination of TRAIL and Smac mimetic induced the synthetic lethal reaction specifically in the mutant-KRAS cells but not in normal lung cells and wild-type <em>KRAS</em> lung cancer cells. Therefore, a synthetic lethal …
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Sequential Optimization for Prospective Customer Segmentation and Content Targeting
… model-based algorithm configuration, or B-SMAC) is developed to automatically and intelligently target online advertisements through iterative batch experimentation. The result, verified through simulation and field experiment, is the maximization and characterization of ad performance over …
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NEW INSIGHTS INTO THE REGULATION OF MITOCHONDRIAL OUTER MEMBRANE PERMEABILIZATION DURING APOPTOSIS
… ( 6 h). Total cellular cytochrome c and Smac were decreased after 24 h of incubation. Interestingly, inhibition of the 26S proteasome by co-treatment of Apaf-1-deficient cells with bortezomib or MG132 led to the robust retention of total cellular cytochrome c and Smac. Combined, the major …
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Desenvolvimento de Modelos Químico - Quânticos para o Estudo de Complexos com Íons Lantanídeos e Actinídeos
… também uma versão do modelo para actinídeos, SMAC/AM1, em particular para o íon Th(IV). No processo de parametrização destes modelos, dois pontos são de fundamental importância: (i) a escolha de um conjunto de treinamento capaz de representar bem o nosso universo de estruturas de complexos e …
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Prognostic biomarkers for head and neck squamous carcinoma
… therapeutics for this disease. TRAIL and Smac mimetics are novel promising anticancer agents. However, their effect on head and neck cancers so far has not been tested. The main objective of this research was to investigate biomarkers which could predict response of head and neck squamous …
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Type I Interferon-Mediated Killing of Cancer Cells with IAP-Targeted Combination Immunotherapy
SMAC mimetic compounds (SMCs) are small molecule antagonists of the Inhibitor of Apoptosis (IAP) family of proteins. Binding of SMCs to the IAPs results in the sensitization of cancer cells to apoptosis in the presence of death ligands, such as tumour necrosis factor alpha (TNFα). I hypothesize …
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Variability and Dynamics of Apoptotic Events in Single Cells
… outer membrane, the release of cytochrome c and Smac from mitochondria, and the activation of the caspase family of proteases. The permeabilization of the mitochondria outer membrane is thought to represent the commitment of a cell to an apoptotic fate, and the subsequent release of mitochondrial …
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The role of TNFAIP1 in regulation of LPS/TNF-ɑ-induced signaling pathway
… HO-1/HMOX1/HSP32, MCL-1, P27/Kip1, or SMAC/Diablo; 5. TNFAIP1 promoter DNA was cloned into pGL3 basic plasmid DNA to determine promoter activity. TNFAIP1 promoter activity was tested via its potential protein-protein interaction using luciferase gene expression. With a MAPK inhibitor, …
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Investigating the Role of the Inhibitor of Apoptosis Proteins (IAPs) in Metastasis Formation
… cell line MDA-MB231 were treated with SM83, a Smac mimetic developed in our laboratory that acts as a pan-IAP inhibitor, and tumour nodules were profiled for gene expression. The analysis revealed that the inhibition of IAPs significantly reduces the expression of SNAI2, a zinc finger …
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Genetic and Biochemical Analyses of the Necessity for Caspase Activation by the CED4-Domain Proteins, APAF-1 and DARK
… site also used by the mammalian anti-IAP protein SMAC to block IAP-caspase interaction. Despite these peptides' failure to allow for reconstitution of caspase activation in vitro, genetic inactivation of IAP's leads to significant activation of caspases in vivo. DARK plays a critical role in …
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Cluster based jamming and countermeasures for wireless sensor network MAC protocols
… of security. We present a new WSN MAC protocol, RSMAC (Random Sleep MAC) that is designed to provide resistance to periodic jamming attacks while maintaining elements that are essential to WSN functionality. CPU, memory and especially radio usage are kept to a minimum to conserve energy while …
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