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Showing 1 to 20 of 21 for “"SKOV3"”.
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Multi-Omics Analysis of 3D SKOV3 Ovarian Cancer Model Cell line Cultured on Novel Silicon-Titanium Diboride Micropatterned Substrate
… metastasis. The ovarian cancer model cell line SKOV3 was cultured on a novel SiTiB2 a physiologically relevant 3D substrates, to generate a cell monolayer, at day three, aggregates at day seven, and disaggregation at day nine. The transcriptomic profiles of the SKOV3 cell lines were analyzed …
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Investigating the role of TRPC3 in the biogenesis of extracellular vesicles
… across a panel of ovarian cell lines, with SKOV3 chosen as the principal line of study. Activating TRPC3 amplified Ca2+ signalling in SKOV3 cells, and significantly increased cell growth and migration, whilst cell invasion was unchanged. In contrast, inhibiting TRPC3 attenuated …
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Immunostimulatory and Cellular Toxic Effects of Single-Stranded RNA On Cancer Cells
… level and cellular toxicity of ssRNAs in SKOV3 cells. In this work, we confirmed that 5'-ppp-ssRNA (~ 60nt) was immunostimulatory and toxic in SKOV3 cells. 5'-ppp-ssRNA (~ 60nt) led to IFN-P induction and apoptosis in SKOV3 cells. Longer 5' ppp- ssRNA tended to have a higher …
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Reactive Oxygen Species are Second Messengers in the Lysophosphatidic Acid Induced Survival Signaling of Ovarian Cancer
… that these signaling cascades are increased in SKOV3 ovarian carcinoma cells when stimulated with LPA. Furthermore, each of these pathways is positively regulated by reactive oxygen species (ROS), which are becoming increasingly recognized as having signaling capabilities. We observed that LPA …
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Tumor-Cell Derived Inhibin, Beta A Is Essential For Adrenergic Effects On Fibroblast Activation
… from non-treated and norepinephrine (NE) treated Skov3 and HeyA8 cells and analyzed for induction of CAF-phenotype by a-SMA expression. Gene and protein expression of cytokines and changes in migratory potential of fibroblasts were assessed.</p> <p><strong>Results:</strong> Among the significantly …
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Atm Signaling to Tsc2: Mechanisms and Implications For Cancer Therapy
… systems including MCF7 breast carcinoma cells, SKOV3 ovarian cancer cells, and various lineages of mouse embryonic fibroblasts, we showed that once activated by reactive oxygen species (ROS), ATM signals to mTORC1 to induce autophagy via the LKB1-AMPK-TSC2 pathway. Targeting dysregulation of …
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Pharmacological regulation and function of store-operated calcium channels
… in the human ovarian adenocarcinoma-derived SKOV3 cells. Blockade of TRPC channel activity by 2-APB, SKF-96365, TRPC pore-blocking antibodies or transfection with TRPC siRNA significantly inhibited SKOV3 cell proliferation. Overexpression of TRPC genes promoted colony growth of SKOV3 cells.It …
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Novel Micropatterned Substrate for Investigation of Ovarian Cancer Aggregate Formation and Response to Epigenetic Drug Therapy
… of cells into 3D aggregates. OVCAR3 and SKOV3 cell lines were used in the absence/presence of pan-genome epigenetic drugs such as suberoylanilide hydroxamic acid (SAHA). The micropatterned substrate supported cell proliferation, migration, and aggregate formation while maintaining …
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Targeting the actin cytoskeleton in HER2+ metastatic cancer cells using a macrolide toxin
… of HER2+ breast (SKBR3, MDA-MB-453) and ovarian (SKOV3) cancer cells led to loss of viability (IC50 values ≤ 64 nM). Sub-lethal doses of MycB treatment caused potent suppression of leading edge protrusions, migration and invasion potential of HER2+ cancer cells (IC50 ≤ 32 nM). In contrast, other …
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Use of a direct, positive selection strategy to generate improved prodrug-activating enzymes for cancer gene therapy
… were generated and their ability to sensitise SKOV3 cancer cells tested. T41G N71S was ~2-fold improved relative to WT NTR. Purified enzymes were also tested with SKOV3 cells, with T41G N71S being ~2.6-fold improved relative to WT NTR. My best enzyme should be considered when planning future …
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Development of Quantitative Molecular Photoacoustic Imaging For Noninvasive Cancer Diagnostics
… shown <em>in vitro</em>, with more signal from SKOV3 cells (i.e., high FRa expression), as well as <em>in vivo</em>, with increased Lipo-JICG contrast enhancement observed from targeted FRa-Lipo-JICG than non-targeted RG-16-Lipo-JICG in mice with SKOV3 ovarian tumors.</p>
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Investigations into the roles of Histamine Receptor H1 and extracellular vesicles in ovarian cancer cell behaviour
… expression was modulated in three OC cell lines (SKOV3, OVCAR3 and OVCAR5), via siRNA transfection, chemical activation (via histamine) or inhibition (via chlorpheniramine) and its effect on cellular behaviour was evaluated through different in vitro assays. Results showed that HRH1: 1) does not …
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The Regulatory Role of Mixed Lineage Kinase 4 Beta in MAPK Signaling and Ovarian Cancer Cell Invasion
… gelatinase activity and invasion in SKOV3 ovarian cancer cells, both of which are cellular responses that require MLK3. Collectively, our data establish MLK4beta as a novel suppressor of MLK3 activation, MAPK signaling and cell invasion.
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Exploring the effects and efficacy of novel cell cycle regulating compounds in aggressive Breast, Ovarian and Cervical Cancer
… breast cancer, MDA-MB 231 (d) Ovarian cancer, SKOV3 (e) Cervical cancer, HeLa. Retinal Pigment The epithelial cell line RPE-1 was used as a control. Western blotting was used to investigate the expression levels of Cdc20 and Cyclin B1 to confirm the compounds were acting on the intended target. …
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The role of p27 phosphorylation in mediating atRA sensitivity of ovarian carcinoma cells
… of p27. Similar results were not observed in SKOV3 cells which are not growth inhibited by atRA treatment. Finally, we demonstrated that overexpression of a mutant of p27 that cannot be phosphorylated on S10 induces a dominant negative effect on the endogenous p27 activity. This dominant …
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Significance of Altered Golgi Apparatus and Cell Fusion Events in Ovarian Cancer Cells
… had no effect on other cell lines (OVCAR3 and SKOV3) tested. Cells exposed to cisplatin during migration showed significantly depolarised Golgi, reduced cell motility and wound healing capacity. Protein trafficking was also significantly reduced in response to cisplatin. Cell-line specific …
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Design and development of novel tools for the screening and identification of inhibitors of HER receptor family and NFR2 for ovarian cancer therapy
… and enhanced cytotoxicity in PEO1, OVCAR3, SKOV3 and MCF7-AREc32 cell lines. This explained the crosstalk mechanism between HER receptor family and NRF2 and the role of NRF2 in drug resistance and as a relevant anti-cancer target which opens up novel avenues of targeting HER receptor kinase …
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Chronic Stress Promotes Tumor Growth Through Increased Bdnf Production and Neo-Innervation
… receptors, which showed that stimulation of the SKOV3 ovarian cancer cell line with norepinephrine (NE) leads to increased expression of several neurotrophins, including brain-derived neurotrophic factor (BDNF). Neurite extension assays showed that media conditioned by ovarian cancer cell lines …
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Targeting Metabolic Adaptations to Anti-Angiogenic Therapy In Ovarian Cancer
… for glutaminase (GLS) expression with the SKOV3 cell line demonstrating the greatest expression and therefore it was utilized for all <em>in vivo</em> experiments. We demonstrated a significant sensitivity of these GLS-expressing cells to GLSi treatment <em>in vitro. </em>AVA treatment …
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A Bayesian Approach to Dose-Response Assessment and Drug-Drug Interaction Analysis: Application to In Vitro Studies
… acid or trichostatin A in the cell lines HEY and SKOV3. This suggests potential new epigenetic therapies in cell growth inhibition of ovarian cancer cells.
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