Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"SKBR3"”.
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The Cell Membrane Proteome of the SKBR3/HER2+ Cells and Implications for Cancer Targeted Therapies
… at investigating the cell membrane proteome of SKBR3 cells, representative of HER2+ breast cancers, and the signaling landscape and cellular responses elicited by the cell membrane receptors when the cells are stimulated with either growth factors or therapeutic drugs. It was hypothesized that …
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The role of receptor tyrosine kinase signalling in HER-2-positive cells and trastuzumab (Herceptin) resistance in breast cancer
… compared to parental cells, while T-resistant SKBR3 cells have significantly elevated IGF-IR levels compared to parental cells (p = 0.026). Targeting IGF-IR with anti-IGF-IR siRNA or an IGF-IR tyrosine kinase inhibitor (TKI) (NVP-AEW541) inhibited the growth of both parental and T-resistant …
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Understanding Acquired Resistance to Lapatinib In Breast Cancer Cells
… acquired resistance, we established a cell line SkBr3-R that is resistant to Lapatinib, from a Lapatinib-sensitive breast tumor cell line, SkBr3. We have characterized the cell lines and demonstrated that Lapatinib resistance in our system is not facilitated by receptor-level activity or by …
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Self-assembled Polymeric Nanoparticles for Targeted Delivery of Anticancer Drugs
… bound specifically and efficiently with SKBR3 breast cancer cells that overexpress HER2 receptors. Anticancer drugs were incorporated into the immuno-nanoparticle system and the drug delivery via an antibody-mediated targeting mechanism was investigated in vitro. First, a protein …
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An In Vitro 3D Model to Evaluate Behaviour of Breast Cancer Cells and Response to Treatment
… 3D cell culture conditions. MDA-MB231 and SKBR3 cells were prepared as spheroids using ultra-low attachment plates and as 'artificial cancer masses' (ACM) by embedding cells in a dense collagen type-I. The ACMs were maintained under flow (150 μL/min) and flow/pressure (550 μL/min, ~19 mmHg) …
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Novel Role of ErbB-2 in Inhibition of Jagged-1-Mediated Trans-Activation of Notch in Breast Cancer
… that trastuzumab or lapatinib treatment of SKBr3 cells</p><p>increased the cell surface protein expression of Jagged-1 by flow cytometry and cell surface biotinylation. Moreover, confocal studies indicated that Jagged-1 and Notch-1 co-localized possibly in early endosomal antigen-1 (EEA-1) …
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Dual mTOR/HDAC Inhibition: Preclinical Development of a Novel Breast Cancer Therapy
… to the single agent treatments in HER2-positive SKBR3 cells, while the viability</p> <p>and apoptosis of non-transformed MCF-10A cells were less affected. Dual MLN0128/TSA treatment also decreased AKT-S473 phosphorylation significantly more than the single agent treatments in all breast cancer …
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Resistance to HER2-targeted therapies in HER2-positive breast cancer
… with HER2-targeting agents showed that SKBR3 cell death could be rescued by iron chelation. HO-1 overexpression in SKBR3 cells revealed increased autophagic flux and resistance to HER2-targeted therapies. Inhibition of autophagy reversed resistance, rendering them susceptible to …
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SMART POLYMERIC DRUG NANOCARRIERS FOR BIOMEDICAL APPLICATIONS
… a normal expression of the HER2 receptor, and SKBR3 which are known to highly over-express the same receptor. Quantitative uptake studies showed good cellular internalization, by both cell lines and an augmented uptake by SKBR3 cells, when the surface was coated with the monoclonal antibody, …
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Quantitative mRNA detection with advanced nonlinear microscopy
… patterns of Her2 mRNA transcript in single SKBR3, MCF7, and HeLa cell are investigated with the SHaSM. SHaSM can successfully differentiate the Her2 mRNA from the nonspecific BTO monomers, and identify more than one transcript in a diffraction-limited spot for SKBR3 cells. Quantification …
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Targeting the actin cytoskeleton in HER2+ metastatic cancer cells using a macrolide toxin
… We found that MycB treatment of HER2+ breast (SKBR3, MDA-MB-453) and ovarian (SKOV3) cancer cells led to loss of viability (IC50 values ≤ 64 nM). Sub-lethal doses of MycB treatment caused potent suppression of leading edge protrusions, migration and invasion potential of HER2+ cancer cells …
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Proteins Involved in HDACi Induced Decay of ERBB2 Transcripts in Breast Cancer
… human breast cancer cell line SKBR3 and an ERBB2 3’UTR mRNA construct and assay designed to precipitate protein complexes, we attempt to identify proteins physically associated with and potentially mediating the polysome-associated ERBB2 mRNA decay pathway. Progress in this …
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Microfluidic Approaches for Probing Protein Phosphorylation in Cells
… developed for the analysis of phospho-proteins. SKBR3 cells were used as a model system for developing and demonstrating the microfluidic chips. The detection and quantification of phospho-proteins involved in MAPK (mitogen activated protein kinase) signaling pathways was achieved at the low nM …
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Polyomavirus Enhancer Activator 3 (PEA3), a Member of the Ets Family of Transcription Factors, Is a Transcriptional Activator of Notch-1 and Notch-4 in Breast Cancer: An Opportunity for Novel Combinational Therapy
… where PEA3 levels are endogenously high. In SKBr3 and BT474 breast cancer cells where PEA3 levels are low, exogenous overexpression of PEA3 significantly increased Notch-4 transcripts. Chromatin immunoprecipitation confirmed enrichment of PEA3 within the promoter regions of both Notch-1 and …
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Combinatorial therapy for triple negative breast cancer and the effect of nanoscale surfaces
… TNBCs along with normal breast cells, MCF-7 and SkBr3, to find the combinatorial index of the drug cocktail. It was found that combination of pentoxifylline, nifuroxazide and amonafide resulted in a combinatorial index of 0.82 in HCC1806 cells. We also investigated the gene and protein expression …
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The Development of Methods for Generating Fc-Conjugates and Antibody-Drug Conjugates
… cytotoxicity in breast cancer cells (SKBR3).
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Modeling Brk/PTK6 expression in the mammary epithelium.
… Brk-knockdown in breast cancer cells (T47D and SKBR3) identified differential phosphorylation of Erk5, and notably, p38 MAPK in response to EGF and heregulin-β1 treatment. These studies illustrate that forced expression of Brk/PTK6 in non-transformed mammary epithelial cells in vivo mediates …
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Characterisation of a novel bioactive complex polysaccharide from a marine invertebrate with potent anticancer and antimalarial activities
… cell line, overexpression of protein HER2- SKBR3 cell line, the hepatoblastoma-derived cell line (Hep G2), chronic myelogenous leukaemia (K562), TNBC and mammosphere formation in breast cancer subtypes, and on malaria has been investigated in vitro using drug sustainable assays. Focusing on …
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Use of Kinase Inhibitors to Illuminate Signaling Pathways in Breast Cancer
In the United States, breast cancer is the most commonly diagnosed cancer and is the second most common cause of cancer-related deaths among women. Among the various subtypes of breast cancer, 25-30% of diagnoses present themselves as human epidermal growth factor receptor 2 positive (HER-2+). …