Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 48 for “"SILAC"”.
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Differential expression profiling of proteomes of pathogenic and commensal strains of Staphylococcus aureus using SILAC
… isotope labeling of amino acids in cell culture (SILAC). Four commensal and pathogenic strains each were grown in the SILAC minimal media (RPMI 1640), containing light (12C) and heavy (13C) form of lysine, respectively, until early stationary growth phase. Various protein fractions, including cell …
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Proteomic Investigation of Endocrine Therapy Resistance in Breast Cancer Investigating the Molecular Mechanisms for SERM Resistant Cell Lines Using SILAC-Based Proteomic Approach
… novel multi-stable isotope labelled amino acids (SILAC) proteomics approach in phenotype-specific breast cancer cell lines resistant to endocrine treatment. Method: In vitro chemo-sensitivity (IC50) was determined for MCF7, T47D, MDA-MB-231, MDA-MB-468, MDA-MB-453, BT-20 and MCF-10A breast cell …
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Development and Application of a quantitative Mass spectrometry based Platform for Thermodynamic Analysis of Protein interaction Networks
… Isotope Labeling by Amino acid in cell Culture (SILAC) quantitation. A solution-based SILAC-SPROX protocol is described in Part III and a SILAC-SPROX protocol involving the use of cyanogen bromide and a gel-based fractionation step is described in Part IV. The SILAC-SPROX-Cyanogen bromide …
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A large scale analysis of chemoattractant induced cytoskeletal dynamics in Dictyostelium discoideum by means of proteomic and imaging approach
… quantitative proteomic technique and adapted the SILAC experimental approach to analyse the fast translocation dynamics of proteins associated with a crude cytoskeleton preparation after stimulation with the chemoattractant in Dictyostelium discoideum. These experiments detected, among many other …
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The role of latent membrane protein 2 in the pathogenesis of Epstein-Barr virus associated malignancy
… isotope labelling amino acid in cell culture (SILAC) to identify interacting proteins which combined with LC-MS/MS analysis was performed and 10 novel LMP2A interactors and 20 novel LMP2B interactors were identified. All these potential interactions require validation but of particular interest …
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Investigating the structure and function of HSV-1 tegument proteins: UL7 and UL51
… screen and quantitative proteomics (SILAC). An interaction between UL51 and the G-Box domain of the centriole protein CPAP was identified by Y2H screen, and validated by immunoprecipitation from transfected cells and in pull-down experiments using recombinant proteins purified from E. …
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A network analysis based proteomic and transcriptomic investigation into HIV-Tat induced neuronal dysfunction and the neuroprotective effect of lithium
… proteomic analysis of HIV-Tat treated SILAC-labelled SH-SY5Y neuroblastoma cells was carried out, alongside transcriptomic analysis of the same system in which 3077 proteins were identified and quantified with 407 proteins and 1074 genes being differentially expressed. Subsequently, …
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Identification of a novel metastasis enhancer, CDCP1, and analysis of its functions during melanoma metastasis
… with high versus low metastatic abilities. Using SILAC (stable isotope labeling with amino acids in culture) coupled with nano-spray tandem mass spectrometry, this work led to the discovery of C̲ub Ḏomain C̲ontaining Protein 1 (CDCP1) as one of those differentially expressed transmembrane …
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Investigating the molecular mechanisms of the interactions between Lactobacillus reuteri strains and intestinal mucus
… labelling with amino acids in cell culture (SILAC), was used to identify novel mucus binding protein candidates. This was achieved through detection of increased cell surface protein expression when L. reuteri was grown in presence of mucins, resulting in induced mucus binding phenotype. …
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Identifying targets of the MAST kinase Drop out through genetic and proteomic analysis in Drosophila melanogaster
… Potential substrates of Dop are identified by a SILAC and phosphoproteomic approach of Drosophila embryos. Substrates identified from this approach may help in understanding the function of Dop during development. In conjunction with the proteomic approach, interactors of dop are also identified …
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A screen for phosphatidylinositol 3,4-bisphosphate and other 3-phosphoinositide effector proteins
… labelling with amino acids in cell culture (SILAC) to differentially label cells stimulated in the absence and presence of the PI 3-kinase inhibitor wortmannin. The integration of these techniques provides a ratio-metric readout, allowing authentically 3- phosphoinositide (3-PI) responsive …
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Development of a Software Package for the Quantitative Analysis of Proteomic Mass Spectrometry Datasets Labelled with Nitrogen-15
… Labelling by Amino acids in Cell culture (SILAC), 15N poses unique challenges for analysis because the level of label incorporation affects not only the relative intensity of signals in MS analysis, but also how that signal is distributed. A computational signal extraction algorithm is not …
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Cell Wall/Surface Proteome of Candida albicans: its Application in Rapid Identification of Yeast Species by Mass Signature and Characterization by in vitro and in vivo Chemical Labelings
… the exposed peptide segments of these proteins. SILAC (Stable Isotope Labeling by Amino acids in Cell Culture) based CWP quantification analyses were performed to monitor CWP accumulation level change in response to hyphae induction. Information on surface exposed peptide segments and regulation …
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Multiple Levels of Regulation of Cyclin-dependent Kinase Inhibitor 1A (p21/WAF-1) by the <i>DDX5 </i>Gene
… p21 expression. In addition, through Pulse SILAC proteomics and western blot validation I have identified a number of potential targets of the DDX5 lncRNA and miRNA species encoded within intron 11.
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Enabling tools for biological analysis : technologies for the study of protein dynamics, detection and interaction
… of proteins with quantitative mass spectrometry (SILAC). Using these arrays we have probed changes in the phosphorylation state of cells in response to activation of the Erb1 and Erb2 receptors. Using our microarray platform we were able to further probe the phosphoproteome for proteins that have …
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Signalling pathway of FBXO7 and its role in hereditary Parkinsonism
… di-GLY capture proteomics combining in vivo SILAC labelling with antibody-based affinity enrichment of “di-GLY remnant motifs”- containing peptides prior to proteomic profiling of the wild-type in comparison to the homozygous R379G Fbxo7 KI ubiquitinome in MEF lysates. The di-GLY remnant …
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IQGAP1 is a novel effector of gonadotropin-releasing hormone receptor signaling
… containing low-density plasma membranes. SILAC (stable isotope labeling by amino acids in cell culture) in combination with mass spectrometry demonstrated that GnRH, within 10 min, altered the association of 87 proteins with this plasma membrane fraction. Ontology analysis revealed that …
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Data analysis in proteomics novel computational strategies for modeling and interpreting complex mass spectrometry data
… on individual peptides, and to identify SILAC modification-containing spectra in a full-scale proteomic analysis. Secondly, matrix decomposition and projection approaches are explored; these use an eigen-decomposition to extract general trends from groups of related spectra. A data …
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Identification of TbRP2-interacting proteins using proximity-dependent biotinylation (BioID)
… in combination with quantitative proteomic (SILAC) techniques, to identify putative TbRP2-interacting proteins in vivo. A selected cohort of these proteins were subsequently interrogated by bioinformatics, localised within the cell using a PCR only (pPOT) YFP-tagging strategy and their …
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The role of JAK1 and JAK3 in CD8<sup>+</sup> effector T cells
… identified using mass spectrometry analysis of SILAC (stable isotope labelling with amino acids in cell culture) labelled CTL. Tofacitinib regulated a selective number of phosphorylation sites, with less than 1.2% of the CTL phosphoproteome significantly regulated by tofacitinib treatment …
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