Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 83 for “"SH2"”.
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Specificity of SH2 domains and protein tyrosine phosphatases
… (PTKs) or have an associated PTK activity. SH2 domains and protein tyrosine phosphatases (PTPs) play essential roles in transmitting these signals. SH2 domains are small domains of V100 amino acids that bind to phospho-tyrosine (pY) in the context of adjacent amino acids. and PTPs counteract …
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Characterizing the Phosphorylation State of Tie2 using SH2 Domain Fusion Proteins
… downstream signaling via their Src homology 2 (SH2) domains. Currently there are no phosphospecific antibodies for Tie2, therefore, identifying critical residues responsible for certain pathways remains difficult. In our study, we aim to use purified SH2 domains of known binding partners to Tie2 …
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Das Interaktom der SH2-Domänen der Proteintyrosinphosphatase SHP2 und ihren krankheitsrelevanten Mutanten
… PI3K pathways. Structurally SHP2 consists of two SH2 domains, a catalytically active PTP domain and tyrosine residues in the C-terminal tail which upon phosphorylation interact with SH2 domain containing proteins. In addition to its enzymatic function, the protein is also capable of exerting …
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Design, synthesis, and thermodynamic evaluation of peptidomimetic ligands binding to the Src SH2 domain
… pYEEI-derived peptidomimetic ligands to the Src SH2 domain were evaluated to investigate the effects of structural changes on protein-ligand binding energetics. The effect of preorganizing the pYEEI ligand into its binding conformation was analyzed by substituting the isoleucine residue with a …
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Protein Tyrosine Phosphorylation in Haematopoietic Cancers and the Functional Significance of Phospho- Lyn SH2 Domain
… of a conserved tyrosine in the Src homology 2 (SH2) domains of Src family kinases, which was frequently observed in human cancer specimens and regulated in cancer-derived cell lines. Using the Lyn SH2 domain as a model, I discovered that when this tyrosine (Y194) is phosphorylated, the domain …
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Disulfide Bridging the Gap between Src and Cortactin: A New Paradigm in SH2 Domain-mediated Signaling
… between Src and cortactin, whereby the Src SH2 domain interacts with cortactin via a disulfide bond formation and that this binding event is necessary for the formation of pro-invasive invadopodia. The work here also defines a paradigm shift in how SH2 domain-containing proteins interact …
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Synthetic Studies on Small Molecule Modulators of Src Homology 2 (SH2) Domain-Containing Inositol 5’-Phosphatase (SHIP)
<p>Small molecule modulators of SH2-containing inositol 5’-phosphatase (SHIP) have recently become a hotly pursued area in medicinal chemistry. Pharmaceutical targeting of SHIP with small molecules has been identified as a new method to directly influence the phosphoinositide 3-kinase (PI3K) cell …
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Biophysical studies of the allosteric regulatory mechanism of Syk tandem SH2 domains interacting with immunoreceptor tyrosine-based activation motifs
… with immunoreceptors through its tandem SH2 domains (tSH2), which contain two SH2 domains connected by interdomain A. The association of Syk with immunoreceptors is regulated by Y130 phosphorylation in interdomain A. The unphosphorylated tSH2 can bind with the doubly phosphorylated …
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Protoplast Fusion for the Production of Intermonoploid Somatic Hybrids in Cultivated Potato
… at the loci). One group of somatic hybrids (SH1, SH2 and SH2B) was of particular interest because it resulted from the fusion of a S. phureja monoploid to a high acetylleptinidine-producing monoploid derived from an F1 hybrid between S. chacoense and S. phureja. The leptine acetylleptinidine (ALD) …
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The intramolecular domain interactions and phosphatase activation mechanisms of Shp2
… in response to growth factors. It contains two SH2 domains (N-SH2 and C-SH2), a phosphatase catalytic domain and a C-terminal tail containing two tyrosyl phosphorylation sites. While the tyrosine phosphorylation of C-terminal tail has been shown to be critical for the proper Shp2 functions, …
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UNIQUE ALLOSTERIC MECHANISM REGULATING PROTEIN-PROTEIN INTERACTION THROUGH PHOSPHORYLATION: A CASE STUDY OF THE CONFORMATIONAL CHANGES IN THE SYK TANDEM SH2 PROTEIN
… kinase comprising three folded domains: two SH2 domains and a catalytic domain. The tandem SH2 domains connected by linker A are key to the regulation of Syk activity. Immune signaling through Syk is initiated by the binding of the tandem SH2 to the dpITAMs found on immune cell receptors. The …
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Protein/Peptide Recognition Modules in Cellular Signaling and HIV Pathogenesis
… utilized by three peptide-recognition domains: SH2 (Src homology 2) domains, PTB (phosphotyrosine binding) domains, and SH3 (Src homology 3) domains. The SH2 domains recognize tyrosylphosphorylated peptides using a binding mode that is conserved among the SH2 family members. In particular, the …
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Funktionelle Charakterisierung von Daughter of Sevenless
… mehrere Tyrosinreste in Konsensussequenzen für SH2-Domänen Bindungsstellen von verschiedenen Proteinen. Die strukturellen Merkmale von Dos und Experimente, die zeigten, daß Tyrosine im Dos Protein nach der Aktivierung von Sev phosphoryliert werden, legen den Schluß nahe, daß Dos zur Familie der …
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Funktionelle Untersuchungen zum PTPN11-Genprodukt SHP2 und zu PTPN11 Mutanten, die dem Noonan-Syndrom zugrunde liegen
… SHP2, die zwei Src-homology-2 (SH2) Domänen und eine Phosphatase-Domäne besitzt. Die N-terminale SH2-Domäne (N-SH2) ist ein konformativer Schalter: entweder interagiert N-SH2 mit der Phosphatase-Domäne (PTP) und hemmt diese, oder sie bindet an ein Phosphotyrosin-Motiv von …
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The Functional Analysis of A Major Tyrosine Phosphorylation Site On Actin
… or ‘readers’, for this modification. Using an SH2 (Src Homology 2) protein domain array, we identify N-terminal SH2 domains of p85, regulatory subunits of Phosphatidylinositol 3-kinase (PI3K), and VAV2, a Rho GTPase guanine nucleotide exchange factor, as phosphorylation-dependent binding …
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FRK cancer-related mutations: Effect on enzymatic activity and cellular processes
… derived from FRK C terminus (ETDSS(pY)SDANN), SH2 consensus binding sequence (HF(pY)ENI), PTEN (PNVEEPSNPEASSS), and BRCA1 (DTYLIPQIPHSHY). The wild type SH2 domain was able of binding to both peptides from the FRK C terminus and the SH2 consensus binding sequence with a Kd of 2.5 M and 0.4 M …
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Molecular and biochemical characterization of suppressor of cytokine signaling-3 (SOCS-3) and SH2-containing phosphatase 1 (SHP-1) in the context of G-CSF) and erythropoietin signaling
In this work interactions of SOCS-3 with the EpoR were studied since there was some evidence from the literature that SOCS-3 would bind to more than one recruitment site on the Epo receptor. For that purpose SOCS-3 was cloned, expressed and purified for subsequent biochemical studies. These …
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Systematic Identification of Interaction Partners of RET Receptor Isoforms
… Two-Hybrid (MaMTH) assay using a library of SH2 domain-containing adaptor and signaling proteins, to screen for interactions with each RET isoform. Through different steps of analysis of MaMTH screen data, we narrowed down RET potential interactors. We complemented these studies by ranking …
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Einfluss des Insulin-ähnlichen Wachstumsfaktors I auf die Androgenrezeptor-Signaltransduktion in Prostatakrebszellen
… Bindung zwischen der C-SH2-Domäne der p85-Untereinheit der PI3-Kinase und dem N- und C-Terminus des Androgenrezeptors nachgewiesen werden. Durch die nähere Charakterisierung dieser Bindungsbereiche mit Hilfe von Peptidarrays und anschließenden Alanin-Substitutionen war es …
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