Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 59 for “"SH-SY5Y-cells"”.
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A study of the neurotoxicity of MPTP and analogs in human neuroblastoma SH-SY5Y cells
… (MPTP) in an in vitro model SH-SYSY human neuroblastoma cells were explored using cytotoxic effects, neurochemical changes and pathological injury as endpoints. The results suggested that: MPTP entered the SH-SYS5Y human neuroblastoma cells through a non-dopamine transport …
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The effects of Parkinson's disease mimetics on the proteasomal and neurofilament systems in SH-SY5Y cells
… the various activities of the proteasome in SH-SY5Y human neuroblastoma cells treated with the PD mimetics MPP+ (a complex I inhibitor) or dopamine. Treatment with these toxins led to dose and time dependent reductions in ATP and glutathione levels and also chymotrypsin-like and …
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DIFFERENTIATION-INDUCED CHANGES IN ANTIOXIDANT CAPACITY AND ANTIOXIDANT RESPONSE IN SH-SY5Y CELLS: ROLE OF NRF2
… antioxidant response. Whereas neuronal cells are post-mitotic and are particularly susceptible to oxidative stress because of high oxygen consumption, astrocytes are mitotic and rely more on glycolytic metabolism. In this study, the difference in antioxidant response and capacity in …
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DIFFERENTIATION-INDUCED CHANGES IN ANTIOXIDANT CAPACITY AND ANTIOXIDANT RESPONSE IN SH-SY5Y CELLS: ROLE OF NRF2
… antioxidant response. Whereas neuronal cells are post-mitotic and are particularly susceptible to oxidative stress because of high oxygen consumption, astrocytes are mitotic and rely more on glycolytic metabolism. In this study, the difference in antioxidant response and capacity in …
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Protective effects of Human Bone Marrow-Derived Mesenchymal Stem Cells on Apoptosis Induced Human Neuroblastoma SH-SY5Y Cells
… using human bone marrow-derived mesenchymal stem cells (hMSCs) is an attractive tool to alleviate neurological disease We investigate whether culture media of hMSCs and coculted hMSC using by transwell have effects against apoptosis induced SH-SY5Ys. SH-ST5Ys were treated with 0.25？？M …
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The effect of glucagon-like peptide-1 (GLP-1) receptor agonists on cell viability, ADAM10 maturation and the proteolysis of ADAM10 substrates in SH-SY5Y cells
… conditions such as AD, as they have been shown to exert neuroprotective properties. Research has also suggested that one of these compounds, exendin-4, can enhance the amount of membrane-associated ADAM10. The current study, therefore, aims to determine firstly; whether GLP-1 analogues can …
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The relationship between the infection of Toxoplasma gondii and Alzheimer’s disease
… on oxidative stress in cultured human brain cellsAs a pilot study, previously prepared slides from 124 brain sections from Alzheimer’s patients and controls were examined for the presence of Toxoplasma gondii cysts. Despite a 10% prevalence of T. gondii in humans in the UK, no cysts were …
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Neuronaalisten kolinergisten nikotiinireseptorien positiivinen ja negatiivinen allosteerinen modulaatio
… opioids, codeine, oxycodone and tramadol in SH-SY5Y cells. SH-SY5Y cells express endogenously α3* and α7-nAChRs. Binding assays were performed with radioactive ligand [3H]-epibatidine. Functional interactions of nicotine and the opioids were studied with 86Rb+- efflux assay. Codeine, …
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Characterising the role of GPR50 in neurodevelopment and lipid metabolism
… metabolism. The work presented in this thesis shows that GPR50 promotes neuronal differentiation. Overexpression significantly increased the number of neurites per cell in SH-SY5Y cells. Further, dendritic branching was enhanced by GPR50 transfection in hippocampal and cortical neurons (DIV …
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Characterisation of the molecular mechanisms of dopaminergic axonal growth and their impairment in Parkinson's disease
… the BMP-Smad transcriptional response in cells overexpressing α-synuclein. α-synuclein impaired both BMPR1B- and Smad1- mediated transcription, however, GDF5 and BMP2 treatment restored α-synuclein-mediated impairments in the BMP-Smad transcriptional response. Histone deacetylase (HDAC)5 …
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Design, development, and evaluation of novel polymer-drug conjugates and interpolymer complexes for the treatment of neurodegenerative disease.
… mechanisms. NM15 (unadjusted and adjusted value) showed significantly enhanced antioxidant activity (p ≤ 0.0001) compared to vanillin. The adjusted value of N5 showed significantly greater AChE and BuChE inhibition (p ≤ 0.0001) compared to HEXNAP. Kinetic and modelling studies revealed that N5 …
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Maternal stress signature and infant neurodevelopment: determining the beneficial effects of human milk oligosaccharides - an in vitro study
… roles in many essential functions, including shaping the gut microbiota, inhibiting pathogen adherence to prevent infection, development of the infant immune response, anti-inflammatory properties, gastrointestinal barrier function and brain development. Recent studies have found that not only …
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Nanotechnology based therapeutic approaches to iron‐induced oxidative stress in an in vitro model of Parkinson’s disease
… protect against rotenone‐induced parkinsonism in SH‐SY5Y cells, and in a co‐cultured hCMEC/D3 ‐ SH‐SY5Y cellular BBB model. In Parkinson’s disease (PD), excess free iron drives the accumulation of toxic hydroxyl radicals within mitochondria of dopaminergic neurons, resulting in sustained oxidative …
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Genomic and non genomic effects of elevated concentration of anabolic steroids in human neuronal cells
… of mu opioid receptor (MOPr) transcripts in SH-SY5Y human neuroblastoma cells. This effect was prevented by the androgen receptor (AR) antagonist hydroxyflutamide. Receptor binding assays confirmed a decrease in MOPr of approximately 40% in nandrolonetreated cells. Treatment with actinomycin …
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Defining the potential of HDAC5 and HDAC9 as therapeutic targets for Parkinson’s disease
… enzymes which regulate histone acetylation, have shown some promise in this regard. For example, pan-HDAC inhibition by pharmacological compounds in PD models have been reported to provide some neuroprotection in PD models. However, given the number of different classes of HDACs (ClassI-IV) and …
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Identification of Roles for Peptidylarginine Deiminases (PADs) In Models of Nervous System Regeneration, Assessing Capacities of Pharmacological PAD Inhibitor Application
… objective of this research study was to establish a consistent in vitro model of CNS injury and neuronal regeneration utilising differentiated SH-SY5Y cells. PAD inhibitors were evaluated for their effects on cell migration and wound healing, using Cl-amidine, AMF30a and GSK199 critical for …
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The role of the E2 copper binding domain in the cell biology of the amyloid precursor protein
… These constructs were expressed in HEK and SH-SY5Y cells and the effects of the mutations on the afore mentioned properties of APP were assessed. The results show that these mutations had little consistent effect on the expression, proteolysis or cellular localisation of APP. Furthermore, …
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Developmental Appearance of Phosphorylated Intermediate Filaments in Nuclei of Glioma and Neuroblastoma Cells
… skeleton in the nucleus of eukaryotic cells, termed the nucleoskeleton, is only vaguely known. Investigations into the role of intermediate filaments (IF) in the nucleus have primarily focused upon lamins, however some studies have suggested that there may be a role for non-lamin …
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In vitro investigation of the molecular relationship between mitochondrial dysfunction and neurogenesis in autism aetiology
… are needed to investigate the molecular relationship between mitochondrial dysfunction and neuronal differentiation as it pertains to ASD aetiology. A key differentially methylated gene was Propionyl-CoA Carboxylase Subunit B (PCCB), a mitochondrial enzyme involved in metabolizing short fatty …
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5-HT1A receptor expression : Studies in postmortem tissue and characterisation of a model system.
… Kd quantifying 5-HT[1A] receptor density. The SHSY-5Y neuroblastoma cell line is a well characterised cell line model used in neurotransmitter studies when differentiated. The 5-HT[1A] receptor couples to Gi proteins inhibiting AC activity and hence mediating a variety of intracellular changes …
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