Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 47 for “"SERCA"”.
-
Herp Reduces ER Calcium Content by Proteasomal Degradation of SERCA
… of Herp interacting proteins, which could be SERCA and the proteasome. Herp may recruit the proteasome from the cytosol to the ER membrane, thereby facilitating the ER associated degradation (ERAD) of SERCA. The proteasome recruitment and the subsequent degradation of SERCA reduce ER lumenal …
-
Examining the Role of Phospholamban Phosphorylation on Interaction with SERCA Using Fluorescence Microscopy
… of the Sarco/Endoplasmic Reticulum Ca2+-ATPase (SERCA) by Phospholamban (PLB) plays a crucial role in normal cardiomyocyte function through controlling the speed and extent of myocyte relaxation. The interaction between PLB and SERCA is altered in many forms of heart failure (HF), making these …
-
An Investigation of the Phospholamban-Serca Regulatory Interaction with Fluorescence Resonance Energy Transfer
… changes in the structure and affinity of the PLB-SERCA regulatory complex in live cells. Using this approach, we have detected a high level of PLB-SERCA interaction even at Ca2+ concentrations known to fully relieve PLB inhibition of SERCA, suggesting that dissociation is not required for relief …
-
Characterizing RyR and SERCA function in the C57 and D2 mdx mouse models of Duchenne Muscular Dystrophy
… and leak, and higher starting myoplasmic Ca2+. SERCA activity (ATP hydrolysis) was lower in D2 mdx mice while higher in C57 mdx mice. These dramatic impairments in SR Ca2+ handling were not attributed to differences in SERCA isoform content or changes in its regulator, sarcolipin. However, under …
-
Probing cardiac calcium regulation using time-resolved optical spectroscopy.
… reticulum calcium adenosine triphosphatase (SERCA) is an integral membrane Ca2+ pump that reuptakes Ca2+ into the lumen of sarcoplasmic reticulum (SR), decreases the cytoplasmic Ca2+ concentration, and initiates relaxation in both skeletal and cardiac muscle. In the heart, SERCA activity is …
-
The Role of Membrane Lipid Composition on Sarco(endo)plasmic Reticulum Calcium ATPase Function in Rat Soleus Muscle
Sarco(endo)plasmic reticulum calcium ATPase (SERCA) is a transmembrane protein whose function is regulated by its immediate lipid environment (annulus). The composition of the annulus is currently unknown or it’s susceptibility to a high saturated fat diet (HSFD). Furthermore it is uncertain if …
-
Mutations in Phospholamban Alter the Structure and Function of the Calcium ATPase Regulatory Complex
… sarco/endoplasmic reticulum calcium ATPase (SERCA). Mutations in PLB that change the PLB monomer-pentamer equilibrium result in dysregulation of SERCA. To determine the structural and regulatory role of the C-terminal residues of PLB in the membranes of living cells, we fused fluorescent …
-
Targeting the calcium ATPase to the endoplasmic reticulum
The sarco/endoplasmic reticulum calcium ATPase (SERCA) pumps calcium from the<br/>cytoplasm into the lumen of the endoplasmic or sarcoplasmic reticulum (ER/SR), removing<br/>excess Ca2+ from the cytoplasm and replenishing ER/SR Ca2+ stores. SERCA is located in<br/>both the ER and the ER-Golgi …
-
Characterization of SERCA function in the hippocampal and prefrontal cortex regions of the brain from C57 and D2 mx mice
… the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) is also highly expressed in the brain and therefore is suspected to play a role in the regulation of neuronal Ca2+. Proper Ca2+ homeostasis is one of the most important factors of cognition, and dysfunctional SERCA has been implicated in …
-
Informing Therapeutic Approaches For Infectious Disease And Heart Disease, Focusing On Cell-Free Synthesis Of Bacteriophages And Calcium Transport By Serca
… to investigate the structural model by which SERCA, PLB, and DWORF regulate intracellular calcium transport in cardiac muscle. I detail the methodology behind purifying and co-reconstituting these membrane proteins in vesicles, and I present the dynamical information of these proteins obtained …
-
Coronary artery disease in metabolic syndrome: a role for the sarcoplasmic reticulum Ca2+ ATPase
… of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) were associated with induction of proliferation. Organ culture of coronary arterial segments revealed that culture-induced medial thickening was prevented by SERCA inhibition with cyclopiazonic acid (CPA). Activation of SERCA with the small …
-
Kontraktilität des isolierten menschlichen Kardiomyozyten. Einfluß von Calcium, Isoproterenol, Stimulationsfrequenz, Stimulationspause und Thapsigargin.
… konnte. So weist die Calcium-ATPase des SR (SERCA) am terminal insuffizienten Herzen eine gestörte Funktion auf. An insuffizienten Ventrikelmyozyten kam es unter niedrigem extrazellulären Calciumangebot zur positiven PPB. Durch Blockade der SERCA mittels Thapsigargin zeigte sich an …
-
PHARMACOLOGICAL MODULATION OF SARCOPLASMIC RETICULUM CALCIUM ATPASE AND CALCIUM RELEASE CHANNELS FOR MUSCLE CELL PROTECTIVE ACTION
… sarcoplasmic reticulum (SR) Ca2+-ATPase (SERCA) and ryanodine receptors (RyRs). However, there are no pharmacological agents that directly and specifically target these two crucial elements required for intracellular SR Ca2+ homeostasis. Dr. Julio A. Copello’s group has previously studied …
-
Glycogen extraction from skeletal muscle sarcoplasmic reticulum: structural and functional implications
… reticulum Ca2+ adenosine triphosphatase (SERCA) and a substantial loss of glycogen phosphorylase and glycogen debranching enzyme (AGL) in amylase-treated samples. Creatine kinase (CK) and pyruvate kinase (PK) contents were increased as a result of both glycogen-extraction conditions. It …
-
Changes in Skeletal Muscle Sarcoplasmic Reticulum Calcium Handling and Regulatory Protein Content in Congestive Heart Failure
… of sarco(endo)plasmic reticulum calcium ATPase (SERCA 1 and SERCA 2 respectively). Calsequestrin (Csq) and calmodulin (CaM) play regulatory roles in calcium handling. Changes in the levels of these proteins could explain alterations in calcium handling and subsequent muscle function. The purpose …
-
Investigation of the endoplsmic reticulum calcium stores for their potential roles in neuroprotection using the NG115-401L neuronal cell line model
… sarco/endoplasmic reticulum Ca 2+ ATPase (SERCA) enzyme. The pharmacological studies involved use of the ginger root compound 6-gingerol, which to date is the best documented agent for activating SERCA enzymes in heart and skeletal muscle. However, in our experiments, gingerol did not …
-
Investigating the role of Neuronatin in neural development
… sarcoendoplasmic reticulum Ca2+-ATPase 2 (Apt2a2/SERCA2), and that Apt2a/SERCA inhibitors cause an increase in cytosolic calcium levels rescuing the ability of Nnat knockdown cells to generate neural cell-types. From these experiments I conclude that Apt2a/SERCA inhibition mimics the function of …
-
The Role of Thada in Energy Homeostasis
… melanogaster to uncouple the heat dissipation of SERCA from its Ca2+ pumping action. We anticipated that this mechanism could contribute significantly to the energy metabolism in endotherms, such as mammals. We envisaged THADA could contribute to the muscle non-shivering thermogenesis—in addition …
Page 1 of 3