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Showing 1 to 20 of 91 for “"SEPHAROSE"”.
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Partial Purification and Characterization of the Calmodulin Sensitive Bovine Brain Adenylate Cyclase
… Upon application of this preparation to CaM-Sepharose, the adenylate cyclase was fractionated into two forms. The major form of the enzyme, 77% of the recovered activity, did not bind to CaM-Sepharose in the presence of Ca('2+) and was insensitive to calmodulin. The remaining 23% adsorbed to …
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Advances in DNA Affinity Chromatography
… for eluting transcription factors from DNA Sepharose columns was characterized. The amount of the lac repressor chimera which eluted from the column was shown to increase with increases in the mobile phase heparin concentration. The elution of the protein was also shown to be dependent on …
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ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΜΕΛΕΤΗ ΙΔΙΟΤΗΤΩΝ ΜΙΑΣ Β-ΓΛΥΚΟΣΙΔΑΣΗΣ ΚΑΙ ΜΙΑΣ Β-ΓΑΛΑΚΤΟΣΙΔΑΣΗΣ ΑΠΟ ΣΠΕΡΜΑΤΑ ΚΡΙΘΑΡΙΟΥ. ΕΦΑΡΜΟΓΗ ΤΩΝ ΕΝΖΥΜΩΝ ΣΤΗΝ ΥΔΡΟΛΥΣΗ ...
… REACTIONS. B-GLUCOSIDASE WAS IMMOBILIZED ON SEPHAROSE, ON RESIN (DUOLITE) AND ON POROUS GLASS BEADS WITH SIGNIFICANT CONSERVATION OF ITS ACTIVITY. THE SEPHAROSE IMMOBILIZED ENZYME ACQUIRED INCREASED THERMOSTABILITY.
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Characterisation of Truncated Mutant Rhodopsin and its Involvement in the Pathogenesis of Retinitis Pigmentosa
… and mutant rhodopsin were purified using 1D4-sepharose separation. Our results supported papers indicating P23A and T4K show lowest degrees of misfolding. Assuming the glycosylation sites are removed we attempted to isolate the truncated species of the ADRP mutant by Con A-Sepharose which …
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ΜΕΛΕΤΗ ΤΗΣ L-ΑΣΠΑΡΑΓΙΝΑΣΗΣ ΣΤΟ ΠΡΩΤΟΖΩΟ T. PYRIFORMIS
… WAS ACHIEVED BY CHROMATOGRAPHY ON PHENYL-SEPHAROSE CL-4B AND SEPHAROSE 6B COLUMNS. THE SPECIFIC ACTIVITY OF THE PURIFIED L-ASPARAGINASE WAS 5.5 UNITS/MG PROTEIN AND THE PURIFICATION FACTORWAS AROUND 300. L-ASPARAGINASE SHOWS A MOLECULAR WEIGHT OF 230,000 DETERMINEDBY CHROMATOGRAPHY ON …
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Some Studies on the Purification and the Properties of Monoamine Oxidase
… Except for tryptamine and serotonin-Sepharose, which irreversibly bound the enzyme, most adsorbents bound little if any of the enzyme. This was true for both detergent (triton X-100 and digitonin) and non-detergent (sonication and perchlorate) soluble enzyme preparations. However, the …
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A Major Cell Envelope Protein Of Escherichia Coli K12: Purification And Properties
… The protein was purified on columns of aspartate-Sepharose (A-S) an affinity matrix previously used for the isolation of dicarboxylate transport components. SEP was the major protein eluted when the A-S columns were equilibrated in low ionic strength buffer (10 mM phosphate containing 5 mM EDTA). …
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Studies of Culture-Derived Soluble Babesia Bovis Antigens: Purification, Characterization and Application in Serodiagnosis
… using horse anti-B. bovis IgG. A Protein A-Sepharose CL-4B adsorbent proved the most efficient as 58 mg of antigen could be purified from 34 ml of culture fluid. Isolated antigens were identified by crossed immunoelectrophoresis.
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Virale Regulatoren der Transkription - Klonierung, Expression und Reinigung von CAEV-Tat, HK022Nun und lambda N(1-53) sowie ihrer Wirtsfaktoren JunbZIP (222-331) und E. coli NusA
… über Kationenaustauscher-Chromatographie an CM-Sepharose und anschließende Hydrophobe Interaktionschromatographie gereinigt werden. Die Ausbeuten an Protein ließ sich durch Verwenden von Heparin-Sepharose als Kationenaustauscher-Material noch weiter steigern. CAEV-Tat zeigte in CD- und …
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Characterizing the Phosphorylation State of Tie2 using SH2 Domain Fusion Proteins
… that Tie2 can bind non-specifically to nickel sepharose when the SH2 proteins were used as antibody mimetics, and was unable to be consistently precipitated in Protein A sepharose when used in conjunction with a monoclonal YFP antibody. Under the latter conditions however we were able to …
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Metabolic Regulation of Cellular Signaling
… first isolated from Xenopus extract using a CaM-Sepharose column. Purified CaM binding proteins were then incubated with CoA-Sepharose in a second purification step and resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. The results indicate the …
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Purification and Characterization of Proteoglycan from Bovine Aortic Endothelial Cells Conditioned Media, and its Interaction with Basic Fibroblast Growth Factor (bFGF)
… and PG-II, by ion exchange chromatography on a Q-Sepharose column using a linear salt gradient (0.15 M to 1.2 M). PG-I and PG-II elute at 0.85M salt and 0.1M salt respectively. BAE PG is primarily composed of heparan sulfate, which is accessible to the digestion of Heparinase I/III and nitrous …
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The molecular and biochemical characterization of cell cycle regulators in Pisum sativum
… a histone H1 kinase activity by p13$\sp{suc1}$-Sepharose affinity chromatography. p13$\sp{suc1}$-associated histone H1 kinase activity is elevated and a set of endogenous proteins is phosphorylated specifically at the S and M phases. Cell cycle changes in the p34 immunoblot pattern are not …
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Identification of Anti-Beta2 Glycoprotein I Auto-antibody Regulated Gene Targets in the Primary Antiphospholipid Syndrome Using Gene Microarray Analysis
… PAPS and IgG was purified using HiTrap Protein G Sepharose columns. Polyclonal anti-2GPI were prepared by passing patient IgG through NHS activated sepharose coupled to human 2GPI. Anti-2GPI preparations were characterized by confirming their 2GPI co-factor dependence, binding to 2GPI and …
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Proteomic Identification of Hemp (Cannabis sativa) Seeds by Tandem Mass Spectrometry and Evaluation of Cytotoxic Activity
… using size exclusion chromatography and blue Sepharose affinity chromatography. Separated proteins were analyzed via Tris/Glycine SDS-PAGE electrophoresis. Protein modification was carried out using performic acid oxidation, followed by Pepsin and tryptic digestion. The resulting tryptic and …
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An extracellular protease from an extreme thermophile
… affinity chromatography on CBZ-D-phe-TETA-Sepharose and Sephadex G75 gel filtration chromatography. The purified enzyme, assigned the title “Caldolysin”, was shown to be homogeneous by a variety of electrophoretic techniques. Two other minor extracellular proteolytic enzymes (totally 12% to …
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Developing as assay to screen inhibitors for various ATP-dependent ligases
… of interaction of the oligonucleotides with Q-sepharose (a strong anion exchanger), was developed to screen inhibitors of DNA ligases from natural product pools as well as chemical libraries. The binding affinities to Q-sepharose resin of a nicked DNA substrate (created from a 30-mer hairpin …
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The Isolation and Characterization of Growth Regulatory Factors Produced by a Herpes Simplex Virus Type 2 Transformed Mouse Tumor Cell Line, H238
… were separated from the H238-CM by heparin-sepharose affinity chromatography into three peaks of mitogenic activity and a fourth containing inhibitory activity for splenocytes. The three peaks of mitogenic activity have been identified based on physiochemical characteristics: the first …
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Development of Bioassays Based on Nucleic Acid Amplification and Next-Generation Sequencing
… antibodies coupled to magnetic particles; (ii) Q Sepharose anion exchanger; and (iii) ceramic hydroxyapatite, Type I. The ITS2 region of the captured pollen-free DNA was PCR-amplified and subjected to next-generation sequencing (NGS). Q Sepharose showed the greatest capacity to capture trace …
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