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Showing 1 to 20 of 54 for “"SELEX"”.
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Development of an RNA aptamer to PD173955N through SELEX
… with a dissociation constant of 1-2 muM using SELEX. The data obtained with selected aptamer suggests that the aptamer could be used to protect PD173955 from aqueous environment. The selection process of the RNA aptamer will be described in detail. The selected aptamer has been reduced to its …
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The Interrogation of Cas9 Aptamers and sgRNA Structures Through SELEX
… Cas9 binding RNA molecules.Here, we use utilize SELEX to both identify novel Cas9 binding RNAs and interrogate the sequence depth of the sgRNA scaffold. First, a SELEX scheme utilizing a nitrocellulose filter binding assay was utilized to identify modified RNA aptamers that bind to Cas9 with …
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In vitro selection of aptamers using quantum dot-assisted capillary electrophoresis SELEX
… Evolution of Ligands by EXponential enrichment (SELEX). The target is incubated with a random sequence oligonucleotide library, and target-bound sequences are separated from the unbound pool to generate a higher affinity library for use in subsequent selection rounds. Capillary electrophoresis …
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Identification of malaria parasite-infected red blood cell aptamers by inertial microfluidics SELEX
… a challenge. Here we develop a novel strategy (I-SELEX) that utilizes inertial focusing in spiral microfluidic channels to stringently partition cells from unbound oligonucleotides. We use I-SELEX to efficiently discover high affinity aptamers that selectively recognize distinct epitopes present …
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A computational approach for the discovery and characterization of oligonucleotide aptamers for protein targets
… evolution of ligands by exponential enrichment (SELEX). SELEX is a powerful experimental method for selecting oligonucleotide aptamers for chemical or biomolecular targets of interest. The SELEX protocol has seen several modifications since its development in 1990, with the primary goal of …
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Microfluidic Selection and Applications of Aptamers
… protocols to the microscale domain. The SELEX process, by which aptamer sequences are selected via isolation from randomized libraries, is a time-consuming and resource-intensive protocol which is being performed with increasing frequency in both academic and private sector laboratories. …
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SELECTION OF APTAMERS FOR INTESTINAL PERMEABILITY TEST.
Since its first description in 1990, SELEX (Systematic Evolution of Ligands by Exponential enrichment) technology has been widely used as an in vitro selection method to evolve nucleic acid ligands, called aptamers, with new functionalities. We have prepared single-stranded DNA aptamers against …
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The Identification and Validation of Novel Aptamers to Glioma
… can be selected against whole cells via cell-SELEX, for production of a targeting ligand, which may also have the secondary advantage of revealing novel biomarkers if the target of the aptamer is elucidated. Previously, the glioblastoma cell line U87MG has been utilised in cell-SELEX for …
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Protein -Dna Interactions Required for Regulation of the Proline Utilization Operon in Salmonella
… of L&barbelow;igands by Exponential Enrichment (SELEX) approach was used to enrich for put control DNA molecules defective in PutA binding in vitro. These experiments identified nucleotide residues that may be important for the specificity of the PutA-put control region interaction. Using the …
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The Selection of Aptamers to CD20 and Their Application as Inhibitors of Complement Dependent Cytotoxicity
… molecule through an iterative process known as SELEX (systematic evolution of ligands by exponential amplification). Aptamers rival antibodies in both binding affinity and specificity. We developed a novel CD20 specific SELEX method, using a lentiviral system to transfect CD20 cDNA into HEK293 …
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The Generation of Novel Aptamers for Glioma and their Pharmacokinetic Profile In Vitro
… target molecules. Aptamers were obtained through SELEX (the Systematic Evolution of Ligands by EXponential Enrichment), an evolutionary selection process that isolates aptamers with a high affinity for the target molecules (low Kd) from a pool of 1014 to 1015 oligonucleotide sequences. In this …
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Using Capillary Electrophoresis for High-Throughput Molecular Screening
… evolution of ligands by exponential enrichment (SELEX) and nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). However, these stand-alone techniques require multiple rounds of selection and produce a limited number of each unique aptamer. SELEX has the added disadvantage of …
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A Microfluidic Approach to Selection and Enrichment of Aptamers for Biomolecules and Cells
… conventional platforms for aptamer generation (SELEX, systematic evolution of ligands by exponential enrichment) is labor-intensive and time consuming. Microfluidic devices have been developed to improve the aptamer enrichment efficiency. However, aptamer generation using these devices is still …
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Dna Aptamers Selected Against Wild-Type Helix 69 Ribosomal Rna And Their Implications In Combating Antibiotic Resistance
… contained 5 × 1014 DNA sequences and was used in SELEX (systematic evolution of ligands by exponential enrichment) (Molecular diversity of the library was reduced to approximately 1 102 after 11 rounds of in vitro selection) experiments. A synthetic construct of H69 was biotinylated and used …
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Detección de cambios en la glicosilación de la PSA asociados al cáncer de próstata: selección de aptámeros y diseño de aptasensores electroquímicos
… Tesis Doctoral se ha diseñado dos metodologías SELEX (Systematic Evolution of Ligands by EXponential enrichment) distintas para dirigir la selección de aptámeros hacia el sitio de glicosilación de la PSA. El primer SELEX se basa en la incorporación de etapas de contraselección frente a una forma …
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Discovery of Peptide and Aptamer Ligands for Targeted Drug Delivery to Hepatic Stellate Cells
… evolution of ligands by exponential enrichment (SELEX). These HSC-specific ligands can be used for diagnosis and therapy of liver fibrosis. The mechanism of fibrogenesis and potential treatments of liver fibrosis are summarized in Chapters 1 and 2. In Chapter 3, we identified HSC-specific …
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Novel therapies for cancer treatment : designing high affinity selective ligands against SIRT1 enzyme
… of current study are to: (i) produce by in vitro SELEX procedures, SIRT1 binding single-stranded DNA (ssDNA) aptamers; (ii) characterise the interactions between selected aptamers and SIRT1 in vitro and determine their equilibrium dissociation constant (KD) values and; (iii) investigate the …
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Aptamers as biosensors
… Evolution of Ligands by Exponential Enrichment (SELEX) and is carried out in vitro. The strength of an aptamer / target binding interaction can be assessed using a variety of different techniques and in this work an Enzyme Linked Immunosorbent Assay (ELISA) was investigated. Other methods …
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I. In vitro selection of aptamers for perchlorate and melamine II. Towards crystallization of DNAzymes III. A highly selective lead sensor based on a classic lead DNAzyme
… discovered in 1990 through a process known as SELEX, systematic evolution of ligands by exponential enrichment. These isolated nucleic acids molecules are termed aptamer, which from the Latin word “aptus” – fit, and they possess ligand binding activity. In 1994, another class of functional …
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Methods of optimization for KCE-based aptamer selection
… also be optimized prior to commencement of KCE-SELEX. Exonuclease targets, such as Exonuclease I (E.coli), recognize the DNA library as a substrate to be degraded. As a result, the enzyme must be inactivated while still maintaining its native three-dimensional structure during aptamer …
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