Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"SELDI-TOF"”.
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Evaluation of SELDI-TOF MS as a tool in colorectal cancer screening
Aim: To assess SELDI-TOF MS technology as a tool for biomarker discovery in the stool and serum of colorectal cancer patients. <br/><br/>Materials and Methods: <br/>1.Initially a technique of analysis was developed and optimised using tumour samples and matched normal mucosa, obtained from the …
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Use of surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) to study protein expression in a rat model of cocaine withdrawal
SELDI-TOF MS is an analytical technology that combines chromatography and mass spectrometry. It is being exploited for the measurement of numerous proteins in serum, less often in organ tissue (rarely in brain). We applied SELDI-TOF MS technology to study protein expression in the brain of rats …
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Differential Proteomic Investigations Of Normal Appearing Gray Matter In Multiple Sclerosis And Control Post-Mortem Brain Tissue.
… Ionization Time of Flight Mass Spectrometry (SELDI-TOF-MS). Principal component analysis (PCA) and hierarchical clustering techniques were used to analyze the differential patterns of SELDI-TOF spectra to reveal clusters of peaks which distinguished MS from control samples. Nine proteins were …
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Proteomische Analyse des Nierenzellkarzinoms: Identifizierung von potentiellen Biomarkern
… verschiedenen proteomischen Methoden untersucht. SELDI-TOF-MS stellt eine neuartige Technologie dar, die bei hoher Sensitivität Proben in kurzer Zeit analysiert. Basierend auf die SELDI-TOF-MS wurde eine Methode etabliert zur direkten Identifikation spezifischer Proteinprofile sowohl von …
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Proteomic Investigation of Salivary Biomarkers in Periodontal Diseases
… of specific putative biomarkers identified by SELDI-TOF MS were confirmed using 1-D PAGE coupled with LC-MS/MS. These biomarkers were then tested using ELISA in the samples that had been initially screened using SELDI-TOF MS as well as in saliva samples in an independent cohort selected …
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Analysis of SELDI mass spectra for biomarker discovery and cancer classification
… time-of-flight mass spectrometry (SELDI-TOF MS) for biomarker discovery and cancer classification. It investigated quantitative measures of reproducibility and found that SELDI protein profiles are affected by sample storage and processing procedure. Two new peak alignment …
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The Application of Innovative High-Throughput Techniques to Serum Biomarker Discovery
… and biomarker discovery. As seen in our initial SELDI-TOF MS and MALDI-TOF MS profiling study of serum for the assessment of breast cancer risk, many profiling strategies typically employ single chemical affinity beads or surfaces to decrease sample complexity of dynamic fluids like serum. …
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The Use of Proteomic Technologies to Identify Serum Glycoproteins for the Early Detection of Liver and Prostate Cancers
… profiling studies such as those involving SELDI-TOF-MS technologies. Though the SELDI approach could distinguish disease from normal by utilizing protein patterns as shown herein with the HCC study of chapter II, it was unable to offer sequence information on the selected peaks, and did not …
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Gen- und Proteinexpressionsanalysen an primären Rattenhepatozyten
… wurde durch 2D-Gelelektrophorese und SELDI-TOF (Surface Enhanced Laser Desorption/Ionisation-Time Of Flight) durchgeführt. Das zweite Ziel war eine Validierungs-studie des beschriebenen Zellsystems. Hierzu wurden Zellen mit dem bekannten Hepatotoxin Acetaminophen behandelt und …
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Protective effect of dietary antioxidants and plant extracts on acute inflammation and hepatotoxicity in vitro
… using immunoprecipitation assay combined with SELDI-TOF MS. Results of present study showed that HepG2 cells provide a simple, sensitive in vitro system for studying the regulation of the negative APP, TTR and RBP under free and inflammatory condition. IL-6, a potent proinflammatory cytokine, …