Global ETD Search
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Showing 1 to 5 of 5 for “"SEC-HPLC"”.
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PNA-protein conjugates for nano-scale modeling of protein aggregates
… and oligomeric forms which were studied by SEC-HPLC and SDS-PAGE. A decrease of anisotropy was exhibited due to homo-FRET following induced formation of dimers and oligomers. In the second model system, fluorescent SNAP-PNA conjugates were controllably assembled on DNA frameworks forming …
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Protection Mechanisms of Excipients on Lactate Dehydrogenase during Freeze-Thawing and Lyophilization
… a glassy, solid matrix during dehydration. The second hypothesis is the "water replacement hypothesis". According to this theory, sugars stabilize proteins by hydrogen bonding and other weak forces of attraction to the polar and charged groups of proteins, to replace the water loss, thus, …
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Development of monoclonal antibody cell culture processes and antibody characterisation
… performed using SDS-PAGE, Western Blotting and SEC-HPLC to assess protein concentration, purity and structural integrity. Stability and forced degradation studies were further conducted to assess the impact of stressful conditions on antibody integrity. Preliminary cell-based assays were …
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Cationic Phenylene Ethynylene Polymers and Oligomers Induce Conformational Changes to Proteins
… level. CD was used to examine changes to the secondary structure of the proteins, fluorescence to examine tertiary structural changes. SDS-PAGE and SEC-HPLC with static light scattering to examine the products of the interaction aggregates or small products then determine the molecular mass of …
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Development and Evaluation of Novel Anti-HER2 Theranostics Against HER2-Positive Breast Cancer
… high performance liquid chromatography (SEC-HPLC), flow cytometry, and antibody internalization. The binding affinities and specificities of the RICs were evaluated by saturation radioligand binding assays, and the results showed high specific HER2 binding. In Chapter 2 (Ketchemen et al. …