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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 360 for “"SDS-PAGE"”.
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In-vitro protein digestibility of human milk and three infant formulas
… sulfate polyacrylamide gel electrophoresis (SDS-PAGE), pH drop and a simultaneous dialysis method. Significant differences (P<0.05) were observed in the in-vitro protein digestibility of human milk and the three infant formulas. Estimated percent in-vitro protein digestibility of each of the …
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Identification and Characterization of Bacterial and Bacteriophage Proteins Via Matrix-Assisted Laser Desorption Ionization (Maldi) Mass Spectrometry
… the techniques for large-scale separation (SDS-PAGE) with very precise, high fidelity approaches to the analysis and characterization of the separated proteins (mass spectrometry). Protein analysis is an underutilized tool that can serve as a valuable alternative to DNA for the …
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In vitro proteolysis of ovine lens crystallins by calpain II : A dissertation submitted in partial fulfilment of the requirements for the Degree of Bachelor of Science with Honours
… and the resulting products were analysed by SDS PAGE. Calpain II was found to cause proteolytic degradation of α- β- crystallins but not γ- crystallins. Degradation of the double α--crystallin bands of 20kDa and 25Da produced three new bands at 22kDa, 19kDa and 18kDa on an SDS PAGE gel. …
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The isolation, identification and detection of Clostridium Difficile
… test for Cl.difficile, to investigate the use of SDS-PAGE for typing isolates and to determine the presence of antibodies against Cl.difficile antigens in the sera of infected patients. Detection of C1.difficile antigens using ELJSA: Using a competitive ELISA, a methodology was developed to …
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Structural studies on the iron storage protein, apoferritin
… were identical, however evidence based on SDS-PAGE and isoelectrofocusing led Drysdale to propose a model with two distinct subunits, the L subunit (m.wt. 19,000) and H subunit (m.wt 21,000) which are combined in different proportions to give various 24-mers (isoferritins). This model has …
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Detection of Sap2 in the secretome of Candida albicans cell wall and secretory mutants
… niekoľkých mutantov C. albicans boli testované SDS- PAGE elektroforézou a zafarbené. Na geli boli pozorované prúžky odpovedajúce BSA a porovnané s kontrolami. Druhá metóda bola vyhodnotená s 96-jamkovými doskami. Výsledky: Bola zistená korelácia medzi absorbanciou a degradáciou BSA. V pilotnom …
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Extracellular Polymeric Substances in Activated Sludge Flocs: Extraction, Identification, and Investigation of Their Link with Cations and Fate in Sludge Digestion
… sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and protein identification by liquid chromatography tandem mass spectrometry (LC/MS/MS), and hemaagglutination (HA)/HA inhibition assays; and 3) investigating the fate of EPS in sludge digestion using SDS-PAGE. Evaluation of prior research …
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A study of the coccoid form and the autolysins of <i>Campylobacter upsaliensis</i>
… not be demonstrated by native (nondenaturing) PAGE. Autolysins were detected, however, by using denaturing SDS-PAGE gels containing either purified <i>E. coli </i> peptidoglycan or whole cells of <i>Micrococcus luteus</i> as the turbid substrate, with subsequent renaturation by treatment with …
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Studies on the mechanism of zinc pyrithione resistance in Pseudomonas aeruginosa PAO1
… of the mechanism of resistance involved SDS-PAGE analysis of outer membranes of biocide-sensitive and biocide-resistant cells, SDS-PAGE LPS analysis,changes in cell surface hydrophobicity, cross resistance, multiple antibiotic resistance, efflux systems and stress …
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Profil protein tanaman kedelai (Glycine Max (L.) Merrill) terinfeksi CPMMV (Cowpea Mild Mottle Virus).
… yang dilakukan dengan metode elektroforesis SDS-PAGE. Identifikasi karakter profil pita protein dimunculkan dengan zimogram. Hasil elektroforesis SDS-PAGE menunjukkan bahwa profil protein tanaman kedelai normal dengan tanaman kedelai terinfeksi CPMMV menunjukkan adanya perbedaan yang jelas. …
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Ruminal degradability of subfractions of protein sources as determined by gel electrophoresis
… analysis of stained polypeptides bands on SDS-PAGE gels. Acidic subunits of soybean glycinin were degraded at a faster rate than basic subunits (.144 vs .104 h⁻¹). Rates of degradation of zein in corn and corn gluten meal were .026 and .015 h⁻¹, respectively. Protein degradability estimated …
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Carbohydrate-Interacting Proteins from Two Nostoc (Cyanobacteria) Species
… sulfate ¡V polyacrylamide gel electrophoresis (SDS-PAGE) and subsequent periodic acid-Schiff (PAS) staining of the resulting gel, the components of this cellular fraction produce a ladder-like pattern, which suggests that the extract may contain glycosylated protein. Analyses of several samples …
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The Effect of Divalent Cations on the Prophenoloxidase Enzyme Cascade Activity in the Freshwater Crayfish Cambarus Latimanus
… weight of the proPO enzyme was estimated using SDS-PAGE and found to be approximately 76 kDa.</p>
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Identifizierung und Charakterisierung IgE- reaktiver Proteine in der Tomate (Lycopersicon esculentum)
… Immunoblot getestet. Detektierte Proteine in der SDS-PAGE wurden mittels LC-MS/MS identifiziert. Dadurch konnten 2 Tomatensamenproteine, die im Immunoblot ein IgE- reaktives Signal zeigten eindeutig mittels Massenspektrometrie identifiziert werden. Diese Proteine sind Legumin und Vicilin. Durch …
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Structure-Function Relationship Studies of the Cytochrome Oxidases
… Upon formation of the P-state a band was seen by SDS-PAGE analysis that migrated faster than the carbonic anhydrase standard (molecular weight 36800). MALDI-TOF mass spectrometry analysis identified this as a single peak with a m/z of 34336.4, which is exactly twice the mass of subunit IV. It is …
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Phospholipid Transfer From Vesicles to High Density Lipoproteins, Catalyzed by Human Plasma Phospholipid Transfer Protein (Liposome)
… with apparent M(,r) = 59,000 and 40,000 by SDS-PAGE. PLTP activity was measured by transfer of ('14)C L-(alpha)-dipalmitoyl PC from egg-PC vesicles to HDL. Activity was enhanced at low pH (4.5) and upon addition of (beta)-mercaptoethanol while Ca('+2) and Na('+) had no effect. E(,act) for …
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Characterization of proteins purified from a beta(1) integrin affinity column: A novel ecm molecule and a muscle-specific integrin alpha subunit
… also showed interesting, anomalous proteins on SDS-PAGE from $\beta$1 affinity purifications from muscle tissue. The possibility that these anomalous proteins represented novel integrin-associated proteins or even new $\alpha$ subunits motivated this work which was to characterize two of the …
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Isolation and characterization of bacterial polyhydroxybutyrate inclusions
… characterized using 13C NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHB inclusions is proposed.
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Separação imunomagnética de cistos teciduais e esporocistos de toxoplasma gondii pelo anticorpo monoclonal K8/15-15 e caracterização de sua proteína alvo por Western Blot e Imunoblot
… gel electrophoresis with sodium dodecyl sulfate (SDS-PAGE). The antigen was processed by Western blot, and bands of 272 and 381 kDa were labeled by IMUNOBLOT. The same bands were observed in a separate SDS-PAGE stained by Comassie blue. The results obtained here will favor the conduction of …
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