Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"SARS-CoV-2 spike protein"”.
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Structural studies of SARS-CoV-2 spike protein and vesicular coats
The first part of this thesis concerns SARS-CoV-2, the causative agent of the coronavirus disease 2019 (COVID-19). The COVID-19 pandemic has raised an immediate need for vaccine and therapeutic development. The SARS-CoV-2 S protein, forming a crown on the surface of the virus envelope, is …
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Aptamers that bind SARS-CoV-2 spike protein for COVID-19 rapid diagnostics and therapeutics
The coronavirus disease 2019 (COVID-19) pandemic continues to severely impact healthcare and socioeconomic circumstances despite advances in rapid diagnostics and vaccines. One hurdle is the emergence of SARS-CoV-2 variants of concern, including the Omicron variant, which has enhanced infectivity …
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Engineering the fusion machinery core of SARS-CoV-2 spike protein for vaccine immunogen design
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-19 without embargo terms
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Computational Analysis of Antibody Binding Mechanisms to the Omicron RBD of SARS-CoV-2 Spike Protein: Identification of Epitopes and Hotspots for Developing Effective Therapeutic Strategies
<p>The advent of the Omicron strain of SARS-CoV-2 has elicited apprehension regarding its potential influence on the effectiveness of current vaccines and antibody treatments. The present investigation involved the implementation of mutational scanning analyses to examine the impact of Omicron …
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Computational Molecular Docking Studies of Small Molecule Inhibitors With the SARS-CoV-2 Spike Protein Variants: In-Silico Drug Discovery Using Virtual Screening and Drug Repurposing Approaches
… severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019 has caused a global public health crisis of nearly unprecedented scale. In the years following the outbreak, the scientific community has mobilized to develop several vaccines and treatments. Drug repurposing as a strategy for …
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Computationally Designed Peptide Binder and Molecular Beacon for SARS-CoV-2
COVID-19 pandemic has caused a catastrophic loss of human life. With only a few approved vaccine candidates and the slow rate of vaccine distribution, particularly in developing nations, there is a need for antiviral candidates and rapid diagnostic solutions. This thesis describes a hybrid pipeline …
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Sub-Picomolar Detection of SARS-CoV-2 RBD via Computationally-Optimized Peptide Beacons
The novel coronavirus SARS-CoV-2 continues to pose a significant global health threat. Along with vaccines and targeted therapeutics, there is a critical need for rapid diagnostic solutions. In this work, we employ a deep learning-based protein design to engineer molecular beacons that function as …
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Structural Basis of Bat and Raccoon Dog Angiotensin-Converting Enzyme 2 (ACE2) Receptor Recognition by Sarbecoviruses
… severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This dissertation investigates the molecular basis of receptor recognition in two species relevant to the emergence of COVID-19: the horseshoe bat (Rhinolophus sinicus) as a potential natural host and the raccoon dog (Nyctereutes …
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Severe Acute Respiratory Syndrome Coronavirus-2 Vaccine Development: A Virus-Like Particle Vaccine Approach
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019 as a highly infectious virus that spread rapidly and was declared a pandemic by the World Health Organization (WHO) in March of 2020.1 SARS-CoV-2 infection causes respiratory disease (Coronavirus Disease; COVID-19), …
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DECIPHERING THE MOLECULAR MECHANISM BEHIND THE SARS-COV-2 FUSION DOMAIN
SARS-CoV-2 is an extremely infectious virus, yet despite a plethora of research the viral lifecycle is still not well understood, particularly the process of membrane fusion. The traditional means by which viral glycoproteins facilitate fusion is that of the six-helix bundle, within which a short, …
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Emerging out of the “blur”: exploration, evaluation and significance of 3D N-glycans’ structure through molecular dynamic studies
… and diverse post-translational modification of proteins, contributing to protein folding, trafficking, structural stability and dynamics, and function. Complex N-glycans are a class of glycans found in eukaryotes, sharing a common pentasaccharide core structure. The functionalization of the arms …
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Evolution of targets at the host-pathogen interface
… – the Severe Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in particular – is presented. Chapter 2: Protein-coding potential of the Mtb genome <br>Using comparative genomics, I investigate the protein-coding potential of globally-distributed Mtb strains. Previous reports had suggested that the …
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Atomistic insights into the structure, dynamics, and regulation of integral membrane proteins
… have become an indispensable tool for uncovering atomistic mechanisms that govern biomolecular function. The unique ability of MD to resolve molecular motions at high spatial and temporal resolution offers mechanistic insights into dynamic interactions that are often inaccessible to …
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Development and Characterization of a Modified Vaccinia Ankara Vaccine Candidate Expressing the SARS-CoV-2 Spike Glycoprotein
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2, SARS-CoV-2. Given the ongoing COVID-19 pandemic and the continued evolution of the virus to escape host immunity, new vaccines and refinement of first-generation vaccines to improve protection against …
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Characterising the magnitude and breadth of T cell responses to SARS-CoV-2 vaccination
Spike protein which serves as the immunogen in the current vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is targeted by the CD4 and CD8 T cells. While most studies frequently measure the magnitude of spike-specific CD4 and CD8 responses, only a few studies have …
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Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli
… AAV capsid assembly, AAV2 and AAV5 VP3 capsid proteins were expressed in E. coli. AAV2 VP3 proteins formed inclusion bodies in E. coli, which were successfully purified and assembled into AAV2 VLPs in a chemically defined reaction. Contrary, AAV5 VP3 developed AAV5 VLPs inside E. coli cells. …
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The gut microbiome: A key regulator of mucosal tolerance, barrier function, and vaccine response
… the microbiota influences the immune response to SARS-CoV-2 spike protein vaccination. Using the ASF mouse model, we demonstrate that ASF mice, despite having a simplified microbiota, exhibit enhanced initial antibody responses to vaccination, particularly in female C3H/HeN mice. This response is …
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Human Antibody Response to Flaviviruses and SARS-Coronavirus-2
… severe acute respiratory syndrome coronavirus (SARS-CoV-2). The first part of this thesis focuses on encephalitis-causing tick-borne flaviviruses, specifically TBEV and POWV. Both TBEV and POWV are emerging human pathogens that cause potentially fatal disease; there are no disease-specific …
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Investigating SARS-CoV-2 immune responses in children
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infec<on causes a highly transmissible respiratory disease, coronavirus disease 2019 (COVID-19), with variable disease outcomes. Children infected with SARS-CoV-2 are more likely to exhibit asymptoma<c or mild illness compared to severe …