Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"S6K1"”.
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Determining S6K1 localisation and interactions with mTORC1 in live cells using fluorescence lifetime imaging microscopy
The S6K1 kinase functions downstream within the mTORC1 pathway to regulate cell proliferation, aging and adiposity. Using GFP technology and advanced imaging, localisation of S6K1 has been established (47% nucleus and 53% cytoplasm). S6K1 strongly (Δτ=200 ps) interacts with the complex scaffold …
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Role of S6K1 in regulating self-renewal of hematopoietic stem cells and propagatoin of leukemia
… activates ribosomal protein S6 kinase 1 (S6K1). To study the role of S6K1 in hematopoiesis as well as leukemogenesis, we used a genetic model of S6K1 deficient mice (S6K1-/-). We found that loss of S6K1 expression in HSCs results in reduction of absolute HSC number in bone marrow (BM). …
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Association of Protein Phosphatase 2A with S6 Kinase Is Regulated In an MTOR-Dependent Manner
… of its two substrates, S6 kinase 1 (S6K1) and 4E binding protein 1 (4EBP1) to promote translation initiation. Many reports have demonstrated the rapid dephosphorylation of S6K1 following treatment with the mTOR inhibitor, rapamycin, as well as by the depletion of amino acids. These …
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Crosstalk Signaling Between cAMP and mTORC1
… kinase inhibitor, renders the phosphorylation of S6K1 and AKT resistant to mTOR inhibitors across multiple cell lines. Moreover, H89 prevented the dephosphorylation of AKT and S6K1 under nutrient depleted conditions. PKA and other known H89-targeted kinases do not alter the phosphorylation status …
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Molecular Mechanism of Insulin Resistance: Role of mTOR Signaling Pathways
… ability of the L545A/L547A mutant to activate S6K1 in cells. Most importantly, when nuclear import was restored in the L545A/L547A mutant by the addition of an exogenous NLS, signaling to S6K1 was rescued. Taken together, these observations suggest the existence of a nuclear shuttling signal in …
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Modulation of plant growth by day length dependent regulation of the TOR signaling pathway
… of TOR, the 40S ribosomal protein S6 Kinase 1 (S6K1) modulates both cell growth and proliferation. Since S6K1 accumulation is modulated by the circadian clock, this thesis investigated how changes in S6K1 levels would affect plant growth when the clock function is impaired by mutation in ZTL …
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mTOR Signaling in Cellular Growth and Skeletal Muscle Maturation
… of mTOR for the activation of newly found S6K1 homologue, S6K2, and for the regulation of skeletal muscle myotube growth. My results showed that S6K2 requires mTOR and its kinase activity for its activation. Amino acid and nuclear-cytoplasmic shuttling of mTOR were shown to be required for …
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AMP-activated protein kinase-mTOR signaling pathway and hepatitis B virus replication
… 증가하였다. Protein phosphatase 2A(PP2A), S6 kinase 1(S6K1)와 eukaryotic translation initiation factor 4E-binding protein 1(4E-BP1)의 발현과 인산화는 HepG2.2.15세포에서 증가하였다. Huh7 HBV WT stable 세포에서는 S6K1의 인산화, PP2A의 발현과 인산화가 증가하였다. AMPK alpha 2가 과발현 될 때 HBV의 core particle과 그 속의 핵산, HBV의 DNA가 감소하였다. HepG2.2.15 세포에 …
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Modulation of Mitochondrial Respiration and Metabolic Signalling in Skeletal Muscle by Caveolin-3
… physiological targets of mTORC1 that include S6K1, 4EBP1 and ULK-1. The reduced activation of the mTORC1/S6K1 axis is also associated with a marked decline in myocellular protein synthesis and a reduction in cellular proliferation. The results presented in this thesis indicate that Cav3 is a …
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The role of mTOR signaling in Alzheimer s disease
… reduction of the ribosomal protein S6 kinase 1 (S6K1), a protein kinase directly downstream of mTOR, improved synaptic plasticity and spatial memory deficits, and reduced the accumulation of amyloid-â and tau, in 3xTg-AD mice. Mechanistically, these changes were linked to reduced translation of …
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Regulatory Roles of Essential Amino Acids, Energy, and Insulin in Mammary Cell Protein Synthesis
… effects on phosphorylation of mTOR, rpS6, S6K1, 4EBP1, eEF2, eIF2α, Akt, and AMPK. These signaling effects on protein synthesis rates were examined with mammary tissue slices. A mathematical representation of the insulin and EAA effects was developed. The effects of supplementing BCAA on …
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The Effect of Betaine Supplementation on Performance and Muscle Mechanisms
… signaling proteins (Total Akt, Akt S473, p70 S6k1, p70 S6k1 T389, and AMPK T172). Following a 14 day washout period, subjects crossed over to the other supplementation protocol and performed the same pre and post AETs.</p> <p><strong>Results: </strong>Following betaine supplementation, there …
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Regulation of skeletal myogenesis by the mTOR signaling network
… manner. Furthermore, systemic ablation of S6K1, a target of mTOR kinase, results in impaired muscle growth but normal nascent myofiber formation during regeneration. Therefore, mTOR regulates muscle regeneration through kinase-independent and kinaseiii dependent mechanisms at the stages of …
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Protein quality and distribution determines anabolic signaling, cellular energetics, and body composition
… the 70 kilodalton ribosomal protein S6 kinase (S6K1). Assembly of the eIF4F initiation complex involves phosphorylation of the inhibitory eIF4E binding protein-1 (4E-BP1), which releases the initiation factor eIF4E and allows it to bind with eIF4G. Binding of eIF4E with eIF4G promotes …
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Effect of early grain feeding of steers on postabsorptive capacity to utilize acetate and glucose
… feeding, phosphorylated:total ratios of rpS6 and S6K1 were greater in EW steers and they had lower acetate clearance rates and greater synthesis rates. The objectives of second study were to assess the effects of early grain feeding on acetate and glucose turnover rates, palmitate synthesis, and …
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Examining the Influence of Muscle Fiber Type on Protein Turnover Signaling in Growing Pigs
… improved translational capacity, enhanced S6K1 phosphorylation, and reduced the abundance of calcium-dependent proteases, RAC feeding had no effect on body or muscle weights. These results suggest that a fiber type transformation without other physiological influences does not alter protein …
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A mechanistic examination of the metabolic regulation of insulin resistance, sarcopenia, and osteoporosis
… acute FSR or total and phosphorylated Akt, mTOR, S6K1 or 4E-BP1 response. The magnitude of the anabolic response to an oral EAA ingestion in healthy older men and women remains intact after 3 months of EAA supplementation.\r\nStudy 3) Older men received continuous weekly testosterone injections …
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Regulation of PPARgamma Activity by mTOR in Adipogenesis
… link between nuclear shuttling of mTOR and S6K1 activation/4E-BP1 phosphorylation. Furthermore, it was found that nuclear shuttling of mTOR regulates mitogen-stimulated rapamycin-sensitive translation initiation. These findings uncover a function for the nucleus in the direct regulation of …
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The Roles of Rap1 in Cancer Metastasis and Pancreatic Islet Beta Cell Function
… through the mTOR and p70 S6 kinase (S6K1) pathway, a known growth-regulatory pathway. This newly defined β-cell axis acts downstream of cAMP, in parallel with the stimulation of both Epac and PKA. Like previous studies on Epac, activation of Rap1 indeed increased glucose …
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Regulation of mTORC1 Signalling and Amino Acid Uptake by Iron Availability in Caco-2 Intestinal Epithelial Cells
… striking reduction in Thr389 phosphorylation of S6K1, Ser240/244 phosphorylation of ribosomal S6 protein and that of 4E-BP1 phosphoryation on Ser65, all of which serve as positive downstream readouts of mTORC1 signalling. The loss in phosphorylation of these mTORC1 targets was reversed upon …
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