Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"S2 cell"”.
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Identification of Peptidyl-prolyl cis/trans isomerases affecting Drosophila circadian rhythms
… behavioral assays 7 D. Plasmids and methods for S2 cell-based assays 8 E. Immunoblotting 8 F. Immunoprecipitation 9 G. Immunohistochemistry 10 H. Quantitative real-time RT–PCR 11 Ⅲ. RESULTS 12 A. PPIases alters Drosophila circadian activity rhythms 12 B. DOD 19 1. Daily cycle of core clock …
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Autoregulation of the Glial Gene reversed polarity in Drosophila melanogaster
During development, cells of the nervous system begin as unspecified precursors and proceed along one of two developmental paths to become either neurons or glia. I seek to understand more about the genes that control this process, focusing on the lesser understood of the cell types, glial cells. …
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Frazzled And Abelson Interact To Regulate The Actin Cytoskeleton In Drosophila
<p>Guidance receptors such as Frazzled affect cell shape and motility by directly or indirectly modulating the cytoskeleton. Fra is particularly needed for the formation of the posterior commissures in a developing Drosophila embryo. The cytoplasmic tyrosine kinase, Abelson Kinase (Abl) enhances …
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Identification of echinus and characterization of its role in Drosophila eye development
… eye results from a coordinated process of cell sorting, differentiation and selective cell death in the retinal epithelium. Mutations in the gene echinus cause supernumerary pigment cells due to insufficient cell death. This study reports the identification of echinus and the …
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Uncovering Reversible AMPylation of BiP Mediated by dFic During ER Homeostasis
… an essential role of this modification in cellular function. Despite the recent discoveries and characterization of a number of bacterial AMPylators and their targets during pathogenesis, the knowledge of AMPylation in eukaryotic system is still elusive. Therefore, the goal of my thesis is …
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Uncovering Reversible AMPylation of BiP Mediated by dFic During ER Homeostasis
… an essential role of this modification in cellular function. Despite the recent discoveries and characterization of a number of bacterial AMPylators and their targets during pathogenesis, the knowledge of AMPylation in eukaryotic system is still elusive. Therefore, the goal of my thesis is …
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Genetic Interactions of Factors That Regulate Alternative Rna Splicing In The Male Germ Line of Drosophila
… to protein functions, and further controls cell differentiation and normal development. Although it is known that most eukaryotic genes produce multiple transcripts in which splice site selection is regulated, how RNA binding proteins cooperate to activate and repress specific splice sites …
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Functional Analysis of Drosophila Integrator Complex In Snrna 3' End Processing
… a functional RNAi screen in <em>Drosophila</em> S2 cells to identify protein factors required for snRNA 3’ end formation. This screen was conducted by using a fluorescence-based reporter that elicits GFP expression in response to a deficiency in snRNA processing. Besides scoring the known …