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Showing 1 to 20 of 399 for “"S. cerevisiae"”.
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Investigation of the [URE3] phenomenon in S. cerevisiae
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Heterodimer formation and tublin function in S. cerevisiae
… polymerization of subunits. However, several S. cerevisiae genes that affect cellular microtubule dependent processes appear to act at other steps in assembly, and to affect polymerization only indirectly. Here we use a mutant [beta]-tubulin to probe cellular regulation of microtubule assembly. …
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Functional dissection of the S. cerevisiae helicase activating factor Cdc45
Eukaryotic DNA replication initiation requires two essential steps: helicase loading and activation. During the G1 to S transition, loaded Mcm2-7 helicases are activated by the S-CDK (S phase cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)- dependent recruitment of the helicase activating …
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The isolation of senescent populations of S. cerevisiae (baker's yeast)
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1995.
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Characterizing the Role of SUMO in Transcriptional Repression in S. cerevisiae
… chaperone CAF-1, is able to bind SUMO in S. cerevisiae. This raises the possibility that CAF-1 plays a role in SUMO-mediated transcriptional repression. Secondly, I used ATAC-Seq to demonstrate that SUMO system inhibition may increase chromatin openness, which could lead to the observed …
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Development of CRISPR/Cas9-based gene drive biotechnology in S. cerevisiae
The budding yeast Saccharomyces cerevisiae is a well-studied eukaryotic model organism for investigating the fundamental aspects of molecular and cellular biology. It also can be used to evaluate the technical feasibility of new technologies in eukaryotic cells. In our lab, we aimed to use …
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DNA-Bindung des Transkriptionsfaktors ABF1 aus S. cerevisiae: Spezifität und Kontaktpunkte
Das ABF1-Protein aus S. cerevisiae ist als ein multifunktioneller, dsDNA-bindender Transkriptionsfaktor bekannt, der nicht nur Transkriptionsprozesse in der Zelle reguliert, sondern auch an der DNA-Replikation und einer Nukleosomen-Rekonfiguration bzw. Änderung der Chromatinstruktur beteiligt ist. …
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DNA damage tolerance and mutagenesis : the regulation of S. cerevisiae Rev1
… and Pol [eta], perform TLS in Saccharomyces cerevisiae. In addition to their contribution to cellular survival after DNA damage, Rev1 and Pol [zeta] are responsible for the majority of spontaneous and damage-induced mutagenesis. Thus mutagenesis, at least for Pol [zeta], is a consequence of a …
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Genome-wide analysis of transcription factors and chromatin in S. cerevisiae
The regulation of transcription initiation is a complex phenomenon, involving DNA-binding activator and repressor proteins, histones and chromatin factors, histone-modifying enzymes, and the general transcription apparatus. Though many of the genes encoding these factors have been identified in …
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Insights into Shugoshin localization and function in S. cerevisiae chromosome segregation
… anaphase II of meiosis. Here we show that in S. cerevisiae cohesins are protected from cleavage during meiosis I in a 50 kb region of chromosomes and Sgo1 localizes to cohesin-associated regions within this region. Interestingly, there is no sequence specificity to the region of Sgo1 association. …
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Chromosome dynamics of the early meiotic cell cycle in S. cerevisiae
In every cell cycle the genetic material must be duplicated and transmitted to the daughter cells. Meiosis is a developmental program that allows a diploid cell to produce haploid progeny. The reduction in chromosome number obtained during meiosis requires specialized mechanisms that are absent …
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Prp45 v regulaci exprese paralogních genů TUB1 a TUB3 v S. cerevisiae
… sestřihový faktor kvasinky Saccharomyces cerevisiae. Lidský ortholog Prp45 - protein SNW1/SKIP - je zapojen do regulace sestřihu pre-mRNA a pravděpodobně ovlivňuje proces transkripce a modifikace histonů. Naše laboratoř odhalila genetické interakce sestřihového faktoru Prp45 nejen s …
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Regulation of the localization of Lte1, a S. cerevisiae mitotic exit activator
… CDKs, in turn, promotes cytokinesis. In S. cerevisiae, mitotic exit is controlled by the Mitotic Exit Network (MEN). In this simple eukaryote, the tight coupling of nuclear migration and mitotic exit is achieved in part by the spatial segregation of Lte1, a positive activator of the MEN, and …
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Regulation of [alpha]-specific gene expression by mating pheromone in S. cerevisiae
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1991.
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
… coli and bovines, the single-celled organism S. cerevisiae has emerged as a well-qualified candidate due to its approachable genetic and fermentation attributes as well as its ability to stably fold disulfide bonded and multi domain proteins. Because S. cerevisiae screens for enhanced protein …
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Insights into the consequences of chromosome gains and losses in S. cerevisiae
… have characterized the G1 delay in aneuploid S. cerevisiae. Aneuploid yeast exhibited a growth defect in G1. Additionally, aneuploid cells displayed a cell cycle entry delay due to delayed accumulation of G1 cyclins. Like other cellular stresses, aneuploidy interferes with the cell's ability to …
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Myosin-Like Proteins in S. cerevisiae: Multifunctional, Structural Components of the Nuclear Envelope
<p>The nuclear envelope (NE) separates the genetic material from the rest of the cell, delimits and defines the nucleus, organizes the intranuclear architecture and serves as a regulator for multiple nuclear processes. In all eukaryotes, filamentous coiled-coil proteins are associated with the …
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Biochemical purification and functional characterization of the She RNP complex from S. cerevisiae
Asymmetric mRNA localization is a widely used mechanism to sort cell fate determinants in development. In the budding yeast Saccharomyces cerevisae ASH1 (for 'asymmetric synthesis of HO') mRNA localization to the tip of the growing bud leads to targeting of Ash1p to the daughter cell nucleus prior …
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Mechanistic studies of the class I ribonucleotide reductases from S. cerevisiae and E. coli.
… of a second R2 subunit is not unique to S. cerevisiae, the potential implication of the heterodimer in the regulation of deoxyribonucleotide synthesis is also considered. In the second part of this thesis, pre-steady state kinetics of the class I E. coli RNR is investigated using …
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The S. cerevisiae calponin homologue SCP1 regulates stability and organization of the actin cytoskeleton
Calponins and transgelins are members of a conserved family of actin-associated proteins widely expressed from yeast to humans. While a role for calponin in muscle cells has been described, the biochemical activities and in vivo functions of non-muscle calponins and transgelins are largely unknown. …
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