Global ETD Search
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Showing 1 to 20 of 26 for “"S-adenosyl-L-methionine"”.
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Methanocaldococcus jannaschii and the Recycling of S-adenosyl-L-methionine
S-Adenosyl-L-methionine (SAM) is an essential metabolite for all domains of life. SAM- dependent reactions result in three major metabolites: S-adenosyl-L-homocysteine (SAH), methylthioadenosine (MTA), and 5'-deoxyadenosine (5'-dA). Each of these has been demonstrated to be feedback inhibitors of …
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Design and synthesis studies of new s-adenosyl-l-methionine analogues
S-Adenosyl-L-Methionine (SAM), which is involved in methyl group transfers, is the second most abundant coenzyme in the human body. It is made from adenosinetriphosphate (ATP) and methionine catalyzed by adenosyltransferase. Methyltransferases (MTs) transfer the activated methyl group from the …
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A Bioinformatics Approach to Identifying Radical SAM (S-Adenosyl-L-Methionine) Enzymes
Radical SAM enzymes are ancient, essential enzymes. They perform radical chemical reactions in virtually all living organisms and are involved in producing antibiotics, generating greenhouse gases, human health, and likely many other essential roles that have yet to be established. A wide variety …
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Biochemical Evaluation of N-Mustard Analogs of S-Adenosyl-L-Methionine in Protein Systems
BIOCHEMICAL EVALUATION OF N-MUSTARD ANALOGS OF S-ADENOSYL-L-METHIONINE IN PROTEIN SYSTEMS
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Synthetic Generation of an Azide-Bearing N-Mustard Cofactor Mimic of S-Adenosyl-L-Methionine
… designed to efficiently mimic <italic>S</italic>-adenosyl-L-methionine (SAM) by incorporating an <italic>N</italic>-mustard and an amino acid moiety surrounding an adenosine core. In addition, an azide functionality was introduced for post-alkylation modification. The crucial factors that led to …
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A fourier transform proton magnetic resonance study of the molecular conformation of S-adenosyl-L-methionine
<p>Contrary to a previous report, S-adenosyl-L-methionine (SAM) affords stable solutions in D<sub>2</sub>O and the <sup>1</sup>H NMR spectrum can be determined. Comparison with the spectra of the model compounds adenosine, L-methionine and L-methionine-S-methyl sulfonium iodide allows complete …
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Structural studies of S-adenosyl-L-methionine radical enzymes involved in tRNA and natural product biosynthesis
Members of the S-adenosyl-L-methionine (AdoMet) radical enzyme superfamily catalyze a myriad of diverse and challenging biotransformations using a [4Fe-4S] cluster and a molecule of AdoMet to initiate radical. In this thesis, we used a combination of crystallographic and biochemical methods to …
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Effects of S-adenosyl-L-methionine (SAMe) supplementation on systemic oxidative stress and inflammatory biomarkers of adult dogs undergoing stress challenges
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
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The Oxidative Deamination of Sulfur Amino Acids by a Bacterial L-Amino Acid Oxidase
… 7.5 for these amino acids were L-homocysteine, L-methionine, S-adenosyl-L-homocysteine, S-adenosyl-L-methionine, L-djenkolic acid, S-ribosyl-L-homocysteine, S-adenosyl-L-homocysteine sulfoxide, L-homocysteine, methionine sulfoxide, L-cysteine and L-cystine.</p> <p>The particulate fraction …
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Viengrandines mažąsias RNR modifikuojančių gyvūnų Hen1 metiltransferazių taikymas RNR žymėjimui ir sekoskaitai /
… C-terminal region, and catalyze the S-adenosyl-L-methionine (AdoMet) dependent methylation of the 2'-O-ribose of the 3'-terminal nucleotide in a subset of small non-coding RNAs. This modification protects small RNAs from degradation, meanwhile the deficiency of the Hen1 protein causes …
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Sensitivity of Radical SAM Enzyme MftC to Molecular Oxygen
<p>MftC is a radical S-adenosyl L-methionine (SAM) enzyme that catalyzes the first step in the production of mycofactocin. It is a ribosomally synthesized post-translationally modified (RiPP) redox cofactor shown to be essential for the survival of bacteria in the <em>Mycobacterium</em> genus in …
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Biochemical characterization of enzymes involved in the biosynthesis of the thiopeptide thiomuracin
… mechanistic investigation of TbtI, a radical S-adenosyl-L-methionine thiazole C-methyltransferase involved in the tailoring modification of thiomuracin, are presented in Chapter 4. Lastly, Chapter 5 discusses the characterization of an ATP and tRNA dependent peptidyl transferase involved in a …
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Mechanistic aspects of thiamine biosynthesis in escherichia coli
… in a reaction catalysed by the radical S-adenosyl-L-methionine (SAM) enzyme, tyrosine lyase (ThiH). The aromatic by-product of this reaction in vivo was unequivocally characterised as p-cresol, a result which supported previous in vitro observations. Development of a reproducible activity …
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Organometallic Chemistry in Fe–S Clusters
… several classes of enzymes, including radical S-adenosyl-L-methionine (SAM) enzymes and enzymes involved in terpene biosynthesis. Due to the transient nature of these intermediates, full characterization of the geometric and electronic structures of enzymatic [Fe₄S₄]–alkyl species has not been …
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The KsgA methyltransferase: Characterization of a universally conserved protein involved in robosome biogenesis
… and structural similarity to a larger group of S-adenosyl-L-methionine dependent methyltransferases, which includes both DNA and RNA methyltransferases. In this document we report that KsgA orthologs from archaea and eukaryotes are able to complement for KsgA function in bacteria, both in vivo and …
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Characterising the response to gliotoxin exposure in Aspergillus fumigatus ΔgliT and ΔgliZ
… data indicate gliotoxin exposure dysregulates S-adenosyl-L-methionine biosynthesis with key enzymes of this pathway, i.e., S-adenosyl-L-homocysteinase and cobalmin-independent L-methionine synthase, eliciting significant changes in their respective expression in response to gliotoxin in A. …
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The roles of redox active cofactors in catalysis : structural studies of iron sulfur cluster and flavin dependent enzymes
… In another system, the characterization of the S-adenosyl-L-methionine (AdoMet) radical superfamily showed the versatility of these cofactors. In this superfamily, which includes over 40,000 unique sequences, [4Fe-4S] clusters are responsible for the initiation of radical chemistry. A recently …
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Release of Histone Lysine Methyltransferases from Rat Brain Chromatin by Nuclease Digestion
… catalyze the transfer of methyl groups from S-adenosyl-L-methionine to specific ε-N-lysyl residues in the N-terminal regions of histones H3 and H4. These enzymes are located exclusively within the nucleus and are firmly bound to chromatin. The chromosomal bound enzymes do not methylate …
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MeCP2 and the Epigenetic Regulation of Excitatory Synaptic Transmission
… by treating neurons with the methyl donor, S-adenosyl-L-methionine. Finally, we addressed the role of histone deacetylation in synapse function by conditionally deleting histone deacetylases (HDACs) 1 and 2 from mature hippocampal neurons. HDAC1 and 2 are present in the transcriptional …
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