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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"RyR2"”.
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The Binding Properties and Functional Consequences of Ryr2-Cam Interaction
… dissertation is to understand the regulation of RyR2. The whole dissertation is composed of two parts. The first part focused on RyR2-CaM interaction. The second focused on synthetic RyR2 domain peptide (DPc10), which worked as a powerful molecular tool for RyR2 functional and structural …
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Functional Importance of Oxidative Post-Translational Modifications of Ryanodine Receptor in Cardiac Sarcoplasmic Reticulum Ca2+ Handling During Oxidative Stress
… contraction in the heart. Ryanodine receptor (RyR2) acts as the primary Sarcoplasmic Reticulum (SR) Ca2+ release channel in the heart. In the event that intracellular SR Ca2+ handling is compromised, both the contractility and electrical excitability of the heart can be altered. As a …
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Adenovirus-mediated gene transfer of FK506-binding proteins FKBP12.6 and FKBP12 in failing and non-failing rabbit ventricular myocytes
… FKBP12.6 bindet an den kardialen RyR (RyR2) und FKBP12 ausschließlich an den skelettalen RyR (RyR1), und damit ausschließlich im Skelettmuskel den RyR reguliert. Eine deutlich höhere Expression von FKBP12 im Vergleich zu FKBP12.6 im Myokard zahlreicher Spezies lassen jedoch Zweifel an …
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In vitro exploration of interactions between junctin and the Ryanodine receptor from skeletal and cardiac muscle
… junctin constructs on RyR1 (skeletal isoform) or RyR2 (cardiac isoform) activity was tested in lipid bilayer experiments with 1 mM luminal [Ca2+] to replicate resting conditions. As found previously, addition of FLjun to the luminal solution increased both RyR1 and RyR2 activity by ~2-3-fold. …
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In vitro exploration of interactions between junctin and the Ryanodine receptor from skeletal and cardiac muscle
… junctin constructs on RyR1 (skeletal isoform) or RyR2 (cardiac isoform) activity was tested in lipid bilayer experiments with 1 mM luminal [Ca2+] to replicate resting conditions. As found previously, addition of FLjun to the luminal solution increased both RyR1 and RyR2 activity by ~2-3-fold. …
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Age-associated changes to calcium handling proteins across the whole heart
… The decline in protein expression of SERCA2a, RYR2 and Caᵥ1.2 supported dysfunctional spontaneous activity in the elderly SA node. In addition there was an age related increase in sensitivity to nifedipine which highlighted the importance of Caᵥ1.2 channels and the compensatory up-regulation of …
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Expression and functional analysis of murine ryanodine receptor type 3
… family of three different isoforms, named RyRl, RyR2 and RyR3. RyRl and RyR2 are highly expressed in skeletal and cardiac muscles respectively, where they localize in the terminal cisternae of the sarcoplasmic reticulum (SR). Although RyRl and RyR2 have been found to be expressed in several other …
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Up-regulation of junctophilin-2 prevents ER stress and apoptosis in hypoxia/reoxygenation-stimulated H9c2 cells
… of JPH2 reduces ryanodine receptor-2 (RyR2)-mediated SR Ca2+ leak and inhibits calcium-dependent calpain activation in H/R-stimulated cardiomyocytes. Lastly, up-regulation of JPH2 prevents endoplasmic reticulum stress in response to H/R. In summary, we demonstrate for the first time …
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Non-genomic control of neuronal calcium signaling by the steroid hormone estrogen leads to neuroprotective effects
… deprivation caused an increase in ERβ and RyR2/3 colocalization, suggesting an interaction would be possible in living neuronal cells. Coimmunoprecipitation confirmed the existence of this interaction. Calcium mobilization experiments showed a correlation between the ERβ/RyR2/3 interactions …
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The Ultrastructural and Abnormal Calcium Handling in Pulmonary Vein Sleeve Cells, Atrial and Ventricular Myocytes During Ageing
… like atrial and ventricular myocytes, express RyR2, Cx40, and Cx43. Whilst RyR2 and Cx43 expressions did not change with age, Cx40 expression was found to deteriorate during ageing. Additionally, EM studies showed significant alterations in mitochondria which increased in size and numbers …
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Diabetes and the Female Heart
… tubules (T tubule), ryanodine receptor type 2 (RyR2) clusters, and filamentous actin (F-actin). Results showed that while diabetic rats developed systemic dysfunction including glucose intolerance and alterations in plasma biomarkers, myocardial energetics were preserved at the trabecula level. …
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Effects of Advancing Age on ER Calcium Regulation in Rat Superior Cervical Ganglia
… not appear to be expressed in the SCG and <em>ryr2</em> and <em>ryr3</em> appear to be the predominantly transcribed isoforms in adult rat SCG. mRNA and protein levels for RyR2 did not change with age, however, the ryr3 mRNA and protein levels significantly increased from 6 to 12 months and …
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Untersuchung einer Calcineurin-bindungsdefizienten FKBP12.6-Mutante in Kardiomyozyten
… gesteuert. Durch die Bindung von FKBP12.6 an den RyR2, die Isoform der Herzmuskelzelle, wird die Offenwahrscheinlichkeit des Kanals und damit der Calciumverlust in der Diastole herabgesetzt. Die durch FKBP12.6 koordiniertere Öffnung benachbarter RyR (coupled gating) bedingt zudem eine verbesserte …
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Ryanodine receptors in calcium signaling pathways
… transcription and real-time PCR to evaluate RyR2 gene expression in transfected/untransfected cells. We also evaluated cytosolic Ca<sup>2+</sup> changes induced by RyR activators or regulators, using fura-2 AM as the Ca<sup>2+</sup> indicator. Successful RyR<sub>2</sub> gene knockdown allowed …
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Arritmogénesis en ratones alimentados con dieta rica en grasas saturadas
… sugerentes de la asociación entre disfunción de RyR2, dieta alta en grasas, y desarrollo de arritmias malignas.
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Molecular mapping of the rabbit atrioventricular node
… ß-subunit SUR2A, and the Ca2+ handling proteins, RYR2, RYR3, NCXI and SERCA2a, there were no significant differences between tissues. In situ hybridisation staining revealed further complexity of the AVN conduction axis tissue. A region of loosely packed atrial tissue immediately adjacent to the …
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Veränderungen der Calcium-Homöostase als mögliche Ursache einer linksventrikulären Funktionsstörung im humanen "hibernating myocardium"
… Austauscher (NCX) und Ryanodin-Rezeptor (RyR2) waren daher Gegenstand der Untersuchungen und sollten im Einzelnen quantifiziert werden. Zusätzlich wurde das Hitzeschockprotein hsp70 untersucht, welches eine wesentliche Rolle in der Phosphorylierungskaskade intrazellulärer Proteinkinasen …
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PHARMACOLOGICAL MODULATION OF SARCOPLASMIC RETICULUM CALCIUM ATPASE AND CALCIUM RELEASE CHANNELS FOR MUSCLE CELL PROTECTIVE ACTION
… help their cause by producing mild activation of RyR2 channels, In addition, we have identify DHBP as a reversible and fast acting RyR inhibitor with potential as template for development of transient inhibitors of spontaneous SR Ca2+ release which may have significant protective action against …
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Functional, Structural and Molecular Alterations in the Heart and Kidney During Diabetes Mellitus
… Ca2+ATPase 2a (SERCA2a), ryanodine receptor (RyR2), Na2+/Ca2+ exchanger, phospholamban (Plb), L-type Ca2+ channel proteins (Cav1.2 and Cav1.3), calmodulin2 (Calm2) and Ca2+/calmodulin-dependant protein kinase II delta (CaMK2d) were significantly (p<0.05) altered in DM compared to age-matched …