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Showing 1 to 16 of 16 for “"Rubisco activase"”.
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Molecular characterization of phosphoribulokinase and rubisco activase from Chlamydomonas reinhardtii
… indicated the presence of a single Chlamydomonas rubisco activase polypeptide which possessed 60 to 65% amino acid sequence identity with the higher plant polypeptides. Chlamydomonas rubisco was less effectively activated by spinach rubisco activase than was spinach rubisco. Genomic DNA blot …
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Creation and analysis of chimeras of spinach and tobacco Rubisco activase
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco: EC 4.1.1.39) catalyzes the initial step in photosynthesis, by combining CO$\sb2$ with ribulose bisphosphate to form two molecules of PGA. Rubisco activase is a protein which regulates the activity of Rubisco in vivo and hydrolyzes ATP. …
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Site-directed mutagenesis of the ATP-binding site in spinach rubisco activase
Spinach rubisco activase has two isoforms derived from alternate splicing with the larger polypeptide containing an additional 4 kD C-terminal domain. Both isoforms, with molecular masses of 41 kD and 45 kD, were synthesized and purified from the transformed E. coli containing the cDNAs. A …
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Mechanism of Light Regulation of Rubisco by Rubisco Activase via Thioredoxin -F in Arabidopsis Thaliana
Rubisco activase is a nuclear-encoded chloroplast protein that is required for the light activation of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) in vivo. In most plants examined to date, there are two soforms of Rubisco activase arising from alternative splicing that differ only at …
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Interactions of ATP, ADP and magnesium ion with rubisco activase and their effects on rubisco activation
Rubisco activase is a chloroplast protein that mediates a greatly enhanced activation of rubisco in the presence of ATP and Mg$\sp{2+}$. The fluorescent dye 1-anilinonaphthalene-8-sulfonate was used to study the binding of rubisco activase with ligands. The results indicated that rubisco activase …
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Rubisco Activase -- a Typical AAA+ Protein With Unique Features, And, PIFI -- a Novel Chloroplast Protein Functioning in Chlororespiration
… named PIFI, with a similar C-extension as activase was identified as functioning in chlororespiration and possibly cyclic electron transport by reverse genetics. In a pifi mutant, which possesses an intact NDH complex, NDH-mediated dark reduction of plastoquinone was disrupted as indicated …
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Regulation of Rubisco in Arabidopsis Thaliana Rca(-) Complemented With Arabidopsis Rubisco Activase Engineered at an ATP Binding Site
Q111E and Q111D showed rates of Rubisco activation greater than 42kD wild type and Q111S at ADP/ATP ratios known to exist in planta. Q111D showed a Rubisco activation rate at night time ADP/ATP greater than the 42kD wild type rate at zero ADP/ATP suggesting an altered response. Measurements of …
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Structure and expression of Spinacea oleracea L. and Arabidopsis thaliana complementary-DNAs encoding ribulosebisphosphate carboxylase/oxygenase activase
Rubisco activase proteins consist of two immunologically related polypeptides in the range of 40- to 47-kDa in most plant species examined. Rubisco activase precursor polypeptides are synthesized on cytoplasmic ribosomes and post-translationally imported into the chloroplast with cleavage of a …
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Identifying the Rubisco Recognition Domain and Critical Arginines in Activase and Engineering Activase With Improved Thermostability for Arabidopsis
Heat sensitivity of Rubisco activase(s) confers a major limitation on leaf photosynthesis at moderately high temperature in Arabidopsis. To overcome this limitation, a thermostable activase for Arabidopsis was generated by engineering the more thermostable tobacco activase with an Arabidopsis …
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Isolation, characterization and expression ofcDNA and genomic sequences encoding ribulosebisphosphate carboxylase/oxygenase activase from barley
… carboxylase/oxygenase (EC 4.1.1.39, rubisco) catalyzes either the carboxylation or oxygenation of ribulose-1,5-bisphosphate (RuBP) to initiate either the Calvin cycle or the photorespiratory pathway. Rubisco needs to be activated in order to catalyze these reactions. Rubisco …
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Low Temperature Induced Signaling and Molecular Responses in Tomato
… during the initial stage of rewarming. By using rubisco activase as a model gene, my study shows that chilling may interfere with the normal expression of photosynthetic genes, by disrupting phosphorylation/dephosphorylation regulatory steps involved in transcription and translation. A diurnal …
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Improving the thermal tolerance of photosynthesis in wheat
… due to a decrease in the activation state of Rubisco. Rubisco activase (Rca) is required to restore and maintain the catalytic activity of Rubisco. Rca has a relatively low temperature optimum; improving its thermal tolerance would maintain Rubisco activity and enhance photosynthesis at higher …
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Investigating photosynthetic mechanisms in model and non-model species
… Finally, in Chapter 5, mutation in Rubisco activase 3 (rca3) showed that this gene is important in maize thermotolerance. The variability in response between the two genetic backgrounds used in this chapter also suggests variation in heat tolerance that could be utilized to study the …
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Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid
… Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Many eukaryotic algae, and a single group of land plants, the hornworts, have an inducible carbon concentrating mechanism (CCM), to overcome these limitations. The efficiency of the CCM is improved when RuBisCO is localised to a …
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Physiological mechanisms of yield improvement in historical U.S. soybean germplasm
… one growing season. Three of these genes encode Rubisco activase which is hypothesized to increase yields by increasing rates of photosynthesis. YEG also include those that encode vegetative storage proteins which are important in the storage and transfer of carbon and nitrogen to sink tissues. …
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Characterization of natural variation in steady- and non-steady-state photosynthesis and its underlying mechanisms to improve productivity in rice (Oryza sativa)
Rice is a staple crop that is critical to food security, especially throughout Asia. Despite tremendous yield increases during the Green Revolution, yields have begun to stagnate in recent decades. Improving the photosynthetic performance of rice presents an opportunity to both increase yields and …