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Showing 1 to 20 of 25 for “"Rnase H"”.
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Foamy Virus RNase H - Aktivität, Struktur und Funktion
… beinhaltet die Protease-, die Polymerase- und RNase H-Domäne. Letztere ist für den Abbau der RNA im entstehenden RNA/DNA Hybrid verantwortlich. Während die FV PR-RT als Monomer vorliegt, besteht die HIV-1 RT aus einem p66/p51-Heterodimer. Erstaunlicherweise ist die isolierte HIV-1 RNase H im …
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Uncovering pathways necessary for the survival of RNase H-deficient Escherichia coli
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-05-01
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C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication
… (TP), spacer, reverse transcriptase (RT), and RNase H, plays critical roles in viral assembly and replication. RNase H domain is required for HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated …
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Σχεδιασμός και Σύνθεση Νέων Ετεροκυκλικών Χηλικών Συμπλεκτικών αναλόγων με αντι-HBV δράση μέσω αναστολής της RNase H του ϊού
… της HBV πολυμεράσης, αυτής της ριβονουκλεάσης Η (RNase H). Σε προηγούμενες έρευνες έχουν αναφερθεί αναστολείς από διάφορες χημικές κατηγορίες, όπως α-υδροξυτροπολόνες, Ν-υδροξυϊμίδια καθώς και κάποια φυσικά προϊόντα. Η παρούσα έρευνα επικεντρώνεται στις Ν-υδροξυπυριδινοδιόνες με πολλούς …
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Characterization of the mechanism of action of new HIV-1 reverse transcriptase-associated ribonuclease H inhibitors
… DNA dependent (DDDP) manner, and Ribonuclease H (RNase H) . The RNase H activity catalyzes highly specific hydrolytic events on the RNA strand of the RNA/DNA replication intermediate, critical to the synthesis of integration-competent double-stranded proviral DNA. Because of its essential role, …
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Struktur-Funktionsbeziehungen und Inhibition retroviraler Reverser Transkriptasen
… Polymerase- und eine C-terminale Ribonuklease H (RNase H)-Domäne. Die Polymerasedomäne kann in die Handflächen-, Finger-, Daumen- und Verbindungssubdomäne unterteilt werden. Letztere verbindet die Polymerasedomäne mit der C-terminalen RNase H. Während der Reversen Transkription schreibt die RT die …
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Thermal adaptation of conformational dynamics in ribonuclease H
… between the two techniques. Ribonuclease HI (RNase H), an 18 kD globular protein that hydrolyzes the RNA strand of RNA:DNA hybrid substrates, has been extensively studied by NMR to characterize the differences in dynamics between homologs from the mesophilic organism \textit{E. coli} and the …
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RNA EXPORT FACTOR ELF1 MAINTAINS GENOME STABILITY IN FISSION YEAST
… hypothesis, I found that when elf1Δ cells lost RNase H, preventing R-loop degradation, mutation rates increased over 6-fold. As understanding sources of genome instability is fairly recent, my research may uncover fundamental mechanisms causing genomic instability in cells with impaired mRNA …
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Structural Studies of the Mammalian RNase H2 Heterotrimeric Complex
RNase H enzymes are endonucleases that specifically catalyze the hydrolysis of ribonucleotides in RNA:DNA hybrids. They are classified into type 1 or type 2 based on amino acid sequence similarity, structure, and biochemical properties. Type 1 RNase Hs require a minimum of four ribonucleotides …
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Étude structurale des riborégulateurs thiB et thiM
… chimique (SHAPE) et la deuxième, enzymatique (RNase H). Dans un second temps, j’ai commencé à mettre au point une technique de transcription couplée à des billes pour étudier la structure de thiM.
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Molekulare Charakterisierung des Retrotransposons Ylt1 der Hefe Yarrowia lipolytica
… Protease (PR), Reverse Transkriptase (RT), RNase H (RH) und Integrase (IN). Das Retrotransposons Ylt1 kommt in verschiedenen Stämmen der Hefe Y. lipolytica vor. Die Transpositionsaktivität dieses Retrotransposon war nachweisbar. Die Aktivität des LTR-Promotors ist von der Kohlenstoffquellen …
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Studies of the MYM-type zinc finger protein ZMYM3
RNase H2 is a trimeric ribonuclease that specifically degrades the RNA moiety of DNARNA hybrids. Mutations in RNase H2 result in Aicardi–Goutières syndrome (AGS), a rare inflammatory disorder notable for being a ‘mendelian mimic’ of congenital viral brain infection. Some AGS-associated mutations in …
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Development of synthetic RNA-targeting catalysts from biostable nucleic acid analogues
… of poor biostability and ability to recruit RNase H, which is likely to reduce specificity in vivo. To address these limitations, we concurrently attempted to evolve novel first-in-class XNAzymes composed of highly biostable chemistries with reduced or no ability to recruit RNase H: locked …
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Studies Toward a General Synthesis of Poly-Substituted Alpha-Hydroxytropolones
… specific medicinal targets: ANT (2")-Ia, HIV RT RNase H, and HBV RT RNaseH and preliminary structure activity relationship studies with β-hydroxytropolones synthesized in this research are outlined.</p>
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Structural determinants of murine leukemia virus (MLV) reverse transcriptase (RT) important for fidelity and drug resistance in vivo
… site, as well as residues residing in the RNase H domain of MLV RT. A number of these MLV RT mutants resulted in statistically significant decreases in fidelity (1.2 to 2.8-fold) whereas two mutants showed a statistically significant increase in fidelity (0.8-fold) relative to wild-type MLV …
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Subunit Interactions of Recombinant HIV-1 Reverse Transcriptase With Mutations at L289
… than wild type RT, and neither mutation affected RNase H activity. Both mutants dissociated into monomers slightly more easily than wild type RT, and both mutants were slower to associate to form dimers than wild type RT. L289P dimers dissociated slightly more easily than L289R dimers and were …
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Structure-Substrate Binding Relationships of HIV-1 Reverse Transcriptase
… The significance of the p51 subunit and the RNase H domain of p66 in terms of their influence on the RNA-dependent DNA synthesis was investigated. Clones of the wildtype HIV-1 RT subunits, p66 and p51, and a recombinant C-terminal deletion mutant, p64, [Barr, P. J. (1987) Bio/Technoloav 5, …
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Selective targeting of MYC by antisense oligonucleotides
… cancer cells with MYCASOs leads to RNase H-mediated cleavage of MYC mRNA and a potent decrease in MYC protein levels. MYC knockdown is accompanied by significant effects on cellular viability and inhibition of cellular proliferation. Furthermore, MYCASO treatment specifically …
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Initial Stages of Hepatitis B Virus Replication
… RNA (pgRNA) is degraded concomitantly by RNase H activity of DNA polymerase. Then, plus-strand DNA is synthesized to double-stranded relaxed circular DNA. Through this HBV DNA replication, immature core particle becomes mature core particle. This mature core particle can either be …
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Foamy Virus Enzymes - Activity, Regulation and Resistance
… is found: proteolysis, DNA polymerization and RNase H activity. In this work, the recombinant PR-RTs from the prototype foamy virus and a simian foamy virus isolate from macaques were purified and compared. The biophysical and enzymatic properties of the two enzymes were similar. However, their …
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