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Showing 1 to 3 of 3 for “"Rna-guided Endonucleases"”.

  1. Methods and models of screening genomic variants

    … link between genotype and phenotype. Advances in RNA-guided endonucleases have enabled such pooled screens in human cells. I first consider a model to understand drivers of immune evasion in a pooled knockout screen conducted in an in vitro model of metastatic melanoma. Next, I discuss strategies …

    mit Repository record for Methods and models of screening genomic variants (opens in a new tab)

  2. Genetic circuits for functional screens of Cas12a guide RNA libraries

    The discovery of CRISPR RNA-guided endonucleases have catalyzed huge technological advancements in the field of synthetic biology, such as the creation of gene drives: genomically encoded CRISPR systems capable of spreading through a wild population. These systems have two components: a …

    mit Repository record for Genetic circuits for functional screens of Cas12a guide RNA libraries (opens in a new tab)