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Showing 1 to 20 of 20 for “"Rna Binding Domains"”.
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NMR studies of RNA binding domains of human lysyl aminoacyl tRNA synthetase
<p>Human lysyl aminoacyl tRNA synthetase (hLysRS) is a multi-functional aminoacyl tRNA synthetase which is primarily involved in protein biosynthesis as well as crucial processes ranging from proinflammatory response to signal transduction. One important, non-canonical function of hLysRS is to …
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Structural insights into RNA binding by NusA and interaction studies of Nun with E. coli Nus factors.
… with C-terminal region of Nun and also that Nun binding to NusA requires NusA ar1 region. On the basis of these results, 1H,15N-HSQC spectra were recorded to monitor the interaction between HK022-Nun and NusA ar1. NMR titration experiments between Nun and NusA clearly showed a lack of chemical …
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Investigation of the roX RNAs and the RNA Helicase MLE in Dosage Compensation in Drosophila melanogaster
… and is composed of the two noncoding roX (RNA on the X) RNAs and at least five proteins, including the RNA helicase Maleless (MLE). MLE contains two highly conserved double stranded RNA binding domains (DRBDs) at the N terminus. We investigated the roles of the roX RNAs and MLE helicase …
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Investigation of the dynamics and conformational changes of the U1A protein
The U1A-SL2 RNA complex is a model system for studying interactions between RNA and the RNA recognition motif (RRM), which is one of the most common RNA binding domains. Despite extensive studies conducted to study the origins of U1A-RNA affinity and specificity, little is known about the kinetics …
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Mutational analysis of the tobacco etch potyvirus cylindrical inclusion protein
… is known to be an NTPase as well as a putative RNA helicase. In a previous study, the CI gene and three mutants were constructed and expressed in the E. coli expression vector pET 11a. In this study the TEV CI gene was cloned into E. coli expression vector pET 15b and five other mutants were …
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Kh Domains on Brain Polyribosomes: FMRP and Nova in Translational Regulation
… syndrome. We demonstrate that several neuronal RNA-binding proteins implicated in human disease are associated with brain polyribosomes, namely the Nova and Hu paraneoplastic antigens and the Fragile X mental retardation protein. We use microarray analysis of polyribosomal mRNAs in knockout …
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Single molecule pull down of double strand RNA binding proteins
RNA molecules are transcribed as single stranded naturally, but with most of them forming in to structures composed of duplex regions, loop, bulge or mismatches. RNAs with double stranded regions, or known as double-strand RNAs (dsRNAs). The class of proteins responsible for processing dsRNAs is …
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Characterization of the Drosophila Scaffold Attachment Factor B (SAFB)
… be organized in a structure consisting of looped domains, which are fixed at their bases to the nuclear matrix or scaffold. SAFB has been identified as a nuclear matrix binding protein in humans. Human SAFBs contain an N-terminal DNA-binding SAP-Box, and an RNA recognition motif (RRM). However it …
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Understanding Recognition of Primary MicroRNAs by Human Drosha/DGCR8
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by suppressing messenger RNAs. The RNase Drosha, as part of the Microprocessor complex, performs the initial processing step in miRNA biogenesis. Drosha, in complex with its partner protein DGCR8, must distinguish its RNA …
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The Domains of The Catalytic Subunit of The Eukaryotic Rna Degrading Exosome, Rrp44P, Have Distinct Functions
… In the cytoplasm, the exosome degrades mRNA in a general mRNA turnover pathway and in several mRNA surveillance pathways. In the nucleus, the exosome processes RNA precursors to form small, stable, mature RNA species, including rRNA, snRNA, and snoRNA. In addition to processing these …
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Design, synthesis, and analysis of conjugated activators for modulation of alternative splicing
… has estimated that >95% of human genes are alternatively spliced, allowing for the production of many protein isoforms from a single gene. Splicing is controlled by SR proteins, factors which bind to exonic splicing enhancers (ESEs) through RNA binding domains and recruit the splicosome via …
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Dissecting the function of MYEF2 in neural stem cells and glioblastoma
… interacting partner. MYEF2 contains multiple RNA binding domains and is expressed primarily in the brain and testis. Here I explored the function of MYEF2 within NSCs and GSCs. I confirm Myef2 is expressed within the brain, however was not specifically enriched in stem cell containing regions. …
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Exploring the functional conservation of muscleblind (Mbl) proteins
… family of proteins involved in many aspects of RNA metabolism, including alternative splicing. Disruption of Muscleblind in several animals lends to a variety of defects and disease, including the multi-systemic disorder Myotonic Dystrophy (DM). Though much is known about the involvement of …
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Identification of Loss of Specific FMRP-RNA Interactions as a Cause of Fragile X Syndrome
… resulting from the loss of function of a RNA-binding protein, Fragile X mental retardation protein (FMRP). This work is devoted to the understanding of functional roles of FMRP in normal neuronal development and in the pathogenesis of the Fragile X Syndrome. Particularly it focuses on the …
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RNA Helicase 1 interacts with an ABCRNAi Transporter: Genetic Interactions with haf-6
… ATP-dependent DEAD/H-box and double-stranded RNA binding domains. rha-1 is orthologous to the Drosophila maleless gene(MLE), an essential component of the dosage compensation machinery that leads to a two-fold increase in transcription of genes located on the single X chromosome of males in …
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Role of DDX RNA helicases in cancer cells
Ester Cannizzaro ROLE OF DDX RNA HELICASES IN CANCER CELLS Abstract DEAD-BOX (DDX) RNA helicases are a large family of proteins characterized by the presence of a DEAD/H (Asp-Glu-Ala-Asp/His) motif. Their main function is to unwind double stranded RNA to promote downstream molecular events. They …
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Characterizing the RNA Editing Specificityof ADAR Isoforms and Deaminase Domains In Vitro
<p>Adenosine deaminases acting on RNA (ADARs) convert adenosine-to-inosine in double stranded RNA. Selectivity of editing sites depends on the sequence of the RNA as well as the secondary structure. Identification of sites of ADAR editing by editome analysis is skewed due to the different abundance …
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Development of Oligonucleotide-directed proximity-interactome MAPping (O-MAP), for characterizing RNA-protein interactions and higher order subnuclear architecture in situ
RNA-protein interactions underscore a broad array of regulatory mechanisms throughout biology, and dysregulation of these interactions contributes to a broad range of human pathologies such as neurodegenerative disorders and many cancers. Recent studies have discovered thousands of RNA-binding …
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Nuclear binding and translation regulation by FMRP through novel associated protein and putative RNA helicase MOV10
… of FMRP has not been widely studied. FMRP is an RNA binding protein that is important for normal neuronal development and maturation as well as proper cognitive development as the lack of FMRP results in fragile X syndrome (FXS). I next move out of the nucleus and study the role of FMRP and …
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Biomacromolecular assemblies: from quinary interactions to diffusion in mammalian cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-08-01