Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"Ring finger"”.
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Investigation of Ring Finger Protein 8 in Mammalian Physiology and Pathogenesis
… hazardous, but are also programmed to occur during normal physiological processes such as immunoglobulin heavy chain (IgH) class switch recombination (CSR), an important mechanism for antibody diversification and specification of immunoglobulin effector function during a humoral immune response …
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Investigation of Interaction between heat shock protein 70.14 (Hsp 70.14) and RBBP6 RING finger domain
… postulated interaction of this protein with the RING finger domain of RBBP6, suggested their role in protein quality control system and chaperone-mediated ubiquitination. Therefore, this study aimed to recombinantly express, purify, characterize and investigate the interaction between Hsp70.14 …
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Transcriptional Regulation of Neurogenic Atrophy-Induced Gene Expression by Muscle Ring Finger-1 and Myogenic Regulatory Factors
… in repression of MuRF1 expression, a MuRF1 RING domain mutant and a MuRF1 c-terminal mutant were created. The mutant constructs were then co-transfected along with MRF expression plasmids and the MuRF1 reporter construct into C2C12 cells and reporter gene activity was assessed. The MuRF1 …
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Recombinant expression, purification and structural characterization of aspergillus niger ring finger domain using nuclear magnetic resonance spectroscopy
… an N-terminal DWNN domain and a conserved RING finger domain. Previous studies determined the solution structure of the human RING finger domain using heteronuclear NMR spectroscopy. A bioinformatics analysis using the human RING finger from RBBP6 as searching bait revealed an unchanged …
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Recombinant expression, purification and structural characterization of Saccharomyces cerevisiae RING finger domain using Nuclear Magnetic Resonance (NMR) spectroscopy
RING finger domain is a domain that is incorporated with other domains to form a large protein known as RBBP6. This is a 250kDa protein that stands for Retinoblastoma binding domain protein 6. It is classified according to different homologues namely RBQ1, PACT, SNAMA, Mpe-1 and P2P-R depending on …
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Biophysical investigations of the interaction between substrate binding domain of Hsp40 and the RING finger domain of Retinoblastoma Binding Protein 6
… disease. Through a yeast-two hybrid study, the RING finger domain of the Retinoblastoma binding protein 6 (RBBP6) and the substrate binding domain of heat shock protein 40 (SBDHsp40) suggested a putative interaction between these two proteins. In this study molecular dynamic simulations were …
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Identifying Novel Interaction Between the E3 Ubiquitin Ligase Ring Finger Protein 20 (RNF20) and the Deubiquitinating Enzyme Ubiquitin Specific Protease 7 (USP7)
… endogenous co-immunoprecipitation, comparing parental and USP7 knockout HCT116 cells, reconstitution assay, overexpression with USP7 or the catalytic mutant USP7, cycloheximide chase and a deubiquitination assay, we show that RNF20 stability is dependent upon USP7 removing ubiquitin …
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ScRING finger protein, Mdm2, MdmX, p53, cancer, ubiquitination, drug discovery, ZINC16046951, University of Zululand, South Africa
… and Dranoff, 2012). Really Interesting New Gene (RING) finger protein is a truncated domain from RBBP6 known to function independently in protein-protein interaction and cellular processing signalling. It is a 10 kDa protein defined by its hallmark residues cysteine and histidine that binds two …
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Analysis of the biophysical and in silico exploration of the interaction between small ribonucleoprotein G and the RING finger domain of retinoblastoma binding protein 6 for the identification of protein anti-cancer compounds
… and interaction patterns of SNRPG with the RBBP6 RING Finger domain with a docking score of 3.40kcal/mol. Using I-TASSER, a potential inhibitor known as (2R)-2-[(2-methyl-5-phenylpyrazol-3-yl) carbonyl amino]-3-napthalen-2-yl-propanoic acid) (4FI) was identified and MM/GBSA binding free energy …
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RAD6-abhängige DNA-Reparatur wird durch Ubiquitin- und SUMO-Modifikation von PCNA reguliert
… sowie das Heterodimer UBC13/MMS2,die über die RING-Finger Prote ine RAD18 bzw.RAD5 an das Chromatin rekrutiert werden,ein.Interessanterweise interagiert neben PCNA auch das SUMO-konjugierende Enzym UBC9 mit den beiden RING-Finger Proteinen sowie mit PCNA selbst und ist so ebenfalls mit dem RAD6 …
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Characterization of Class D VPS Proteins
… The Vps8p protein contains a C-terminal H2 RING finger motif, a domain often associated with E3 ubiquitin ligase activity. In vitro analysis reveals that a Vps8p fragment containing this domain has this activity. Deletion of the RING finger reveals that the endocytic marker Ste3p accumulates …
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A yeast 2-hybrid screen to identify and characterize interaction partners of the cancer associated protein retinoblastoma binding protein 6
… the N-terminal ubiquitin-like DWNN domain and RING finger domain, were used as baits in a yeast 2-hybrid screen of a human testis cDNA library. Putative interactors were verified using in vitro and in vivo immunoprecipitation assays. The RING finger domain was shown to interact with …
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PROTEASOME-DEPENDENT ENTRY OF HERPES SIMPLEX VIRUS
… with cellular proteasomes. ICP0 has a RING finger domain with E3 ubiquitin ligase activity that is necessary for its IE functions. The fundamental and functional properties of ICP0 that is present in the virion tegument layer have not been well characterized. For these reasons, I sought …
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TRIM22/Staf50 - a novel target gene of the tumor suppressor p53
… of TRIM proteins (RBCC proteins) consisting of a RING finger, a B-box, a coiled-coil and a variable C-terminal. The TRIM proteins have been shown to be involved in cellular processes like virus infection, ubiquitination, differentiation and apoptosis. I have found that overexpression of TRIM22 …
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Functional Interactions Between Par-1b Protein Kinase and RNF41 E3 Ubiquitin Ligase
… along with associated proteins. Nrdp1/RNF41, a RING finger E3 ligase was identified in their screen along with 14-3-3 and aPKC. I found that Par-1b binds to RNF41 and I identified RNF41 as a novel cell polarity determinant. My work demonstrated that phosphorylation of RNF41 on S254 by Par-1b is …
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Genetic analysis of programmed cell death in Drosophila melanogaster
… In addition, our mutants indicate that Diapl's RING finger domain, a domain found in proteins that function in ubiquitination, is required for inhibition of Rpr- and Grim-induced death but not Hid-induced death.
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The Generation of Alternative Transcripts as a Means of Regulating Phosphorylation in Sonic Hedgehog Medulloblastoma
… isoforms that include or exclude exons harboring critical phosphorylation sites, offering a potential mechanism for regulation at the mRNA level. The sonic hedgehog (SHH) subtype of medulloblastoma (MB), the most common malignant pediatric cerebellar tumor, arises from granule neuron …
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Functional Analysis of KAP1 Requirements for HIV-1 Transcription and Latency Reactivation
… reporter assay, I genetically defined the RING finger domain and an Intrinsically Disordered Region as key activating features. However, KAP1 acute loss in a physiological relevant model, reveals limitations compared to chronic silencing of KAP1 in the same system. I also identified a …
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The Discordant Development of Sexual Orientation in Identical Twins
… discordant sexual orientations. Finally, differing intrauterine environments during the early development could result in a discordant development of sexual orientation in identical twins. This includes varied prenatal hormonal exposures between twins. Chapter 4 highlights a putative biomarker …
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