Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Ricin"”.
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In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors
Ricin is a dimeric enzyme found in the castor bean plant. It is extremely toxic with a fatal dose for humans ranging from 0.1-1.0 ug/kg. This has lead to its use as a biological weapon. Cell death is caused when ricin ceases the protein synthesis by removing a specific adenine (A-4324) of the GAGA …
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Intradermal Administration of RiVax, a Ricin Vaccine
Ricin toxin is a CDC Level B Biothreat due to its extreme toxicity and ease of production. The most effective method for minimizing ricin toxicity in humans is prophylactic vaccination. We have previously described the efficacy and safety of RiVax, a recombinant mutant of ricin A chain (RTA). RiVax …
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Structure based design of a ricin antidote
Ricin is a potent cytotoxin easily purified in large quantities. It presents a significant public health concern due to its potential use as a bioterrorism agent. For this reason, extensive efforts have been underway to develop antidotes against this deadly poison. The catalytic A subunit of the …
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Ricin B Chain-Insulin Fusion Protein Immunomodulation of Type 1 Diabetes
… was linked to the non-toxic B chain of ricin—an enterocyte-binding plant lectin that possesses intrinsic cell-delivery and immunostimulatory properties. The human proinsulin (INS) ricin toxin B chain (RTB) fusion protein was expressed in <em>Escherichia coli </em>and purified for use in …
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B CELL EPITOPE MAPPING OF RIVAX, A CANDIDATE RICIN VACCINE ANTIGEN
Ricin toxin’s enzymatic A subunit (RTA) is a 267 amino acid RNA N-glycosidase that depurinates a conserved adenine residue of 28S rRNA, resulting in ribosome arrest and apoptosis. One of the leading subunit vaccine candidates for ricin is RiVax, a two point mutant (V76M, Y80A) of RTA. RiVax has …
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The characterisation of a recombinant cytosolic shuttle based on ricin toxin
… macromolecules to the cytosol, one example being ricin toxin (RT). In this thesis, a cytosolic drug delivery system based upon attenuated RT was characterised. The toxicological consequences of mutating RT A chain (RTAC) amino acids: Glu177, Ala178, Ala179, Arg180, Phe181, Gln182 to Gly (mut) or …
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Rationalising the effect of guanine-based Ricin inhibitors using computer simulations
… a therapeutic agent. the reaction mechanism of ricin is not known and so rational design is limited, resulting in no effective inhibitor being found for this enzyme. An important step towards understanding the reaction mechanism and the design of effective inhibitors is the complete …
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Simulation and modelling to develop a nanopore-based device for ricin detection
… devices to detect the bioterror agent, ricin. The optimal signal-to-noise ratio occurred when ricin could occupy and interact in a stable manner within a pore cylinder that was similar in size, as predicted by the steric exclusion model of current blockage. Interestingly, under certain …
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Plant-derived Murine IL-12 and Ricin B-Murine IL-12 Fusions
… equivalency to endogenous mouse IL-12. Ricin B (RTB), the non-toxic carbohydrate-binding subunit of ricin, directs uptake of ricin into mammalian cells and the intracellular trafficking of ricin A, the catalytic subunit of ricin. RTB's function suggests that it may work as a molecular …
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Selection of castor with divergent concentrations of ricin and RCA using radial immunodiffusion
… limiting factors in the production of castor (Ricinus communis L.) is the presence of two toxic proteins, ricin and RCA. Ricin and RCA's presence in the endosperm of the seed creates potential health hazards to both producers and processors. The objectives of this research were to determine the …
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Receptor mediated targeting of liposomes
… using either α-melanocyte stimulating hormone or Ricin-B-chain as ligands for promoting the binding of liposomes to cells. α-melanocyte stimulating hormone has been conjugated to liposomes and to Ricin-A-chain via the Lys₁₁ residue without significant loss of biological activity. The resulting …
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Ex vivo elimination of normal and malignant T cells from bone marrow grafts by ricin A-chain immunotoxins
Contains fulltext : mmubn000001_074176420.pdf (Publisher’s version ) (Open Access)
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Expression strategies for plant-based production of a vaccine adjuvant
… to facilitate an appropriate immune response. Ricin, a plant toxin that survives the human digestive process, has been proven to stimulate an immune response and could therefore serve as a suitable adjuvant. The long-term goal is to produce a vaccine that protects against the disease entamoebic …
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Binding Determinants of HIV-1 REV RNA to the Pokeweed Antiviral Protein
… that removes an adenine residue from the sarcin/ricin loop (SRL) of rRNA through a process called depurination. PAP has also been shown to depurinate the ORF of Rev RNA of HIV-1 in vivo without causing toxicity. The sequence and structure of Rev RNA that PAP interacts is identified and compared …
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Application of silicon photonic microring resonators to the study of surface bioconjugation and protein capture agent binding kinetics
… of two single domain antibodies targeting ricin toxin are evaluated simultaneously. In this preliminary study, single domain antibodies are found to be much more highly specific to ricin as compared to the antibody against the same target. Optimization of experimental conditions is still …
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Development of novel bio/nano interfaces for materials science and biomedical applications
… drugs, siRNA cocktails, and the A-chain of ricin toxin, to cancer cells without affecting the viability of normal cells (e.g. hepatocytes, endothelial cells, and immune cells). siRNA cocktails and ricin A-chain, when delivered to cancer cells via targeted VLPs, induce apoptosis within 48 …
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Engineering of the RTB Lectin as a Carrier Platform for Proteins and Antigens
… galactose/N-acetyl-galactosamine specific lectin ricin B-chain (RTB) is especially attractive in possibly fulfilling a carrier role due to its well-characterized endocytotic trafficking and its efficacy over a wide range of cell types. By producing RTB recombinantly in plants it is possible to …
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Characterization of KPAP, a Novel Pokeweed Antiviral Protein Isoform
… which remove an adenine base from the sarcin/ricin loop (SRL) of the 28S rRNA in eukaryotes. This depurination results in protein translation inhibition leading to a concentration-dependent cytotoxic consequence. Many RIPs in the plant kingdom are being discovered with new genomic sequencing …
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Computational prediction of allosteric nucleic acids
… adapt this format to the potential detection of ricin, but were unsuccessful. In order to better evaluate rational designs, we attempted to use computational modeling methods. Again, aptamer biosensors have previously been engineered based on ligand-induced reorganization of secondary structure …
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Dietary Plant Lectins May be an ‘Unknown Etiology’ in Parkinson’s Disease and Dietary Bioactive Compounds Affect Lifespan and Fat Storage Aspects of Caenorhabditis Elegans
… to some Parkinsonism. Neurotoxin botulinum or ricin can trans-synaptically transport in nervous system. Our hypothesis 1: dietary plant lectins might be responsible for the “unknown pathogen” causing PD. Pomegranate juice (PJ) have antioxidant and anti-obesity effects. Our hypothesis 2: PJ …
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