Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Ribotyping"”.
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Bacterial Source Tracking in the Sinking Creek Watershed Using Antibiotic Resistance Analysis and Ribotyping.
… antibiotic resistance analysis (ARA) and ribotyping, to determine the sources of bacteria isolated from Sinking Creek. Based on the results of this study, ARA and ribotyping are not useful techniques for identifying sources of fecal pollution in Sinking Creek. ARA classification rates were …
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A Study on the Prevalence of Gram-Negative Bacteria Isolated from Bulk Tank Milk with Special Reference to Pseudomonas Fluorescens
… profiles) and genotypic (plasmid and 16S-23S PCR ribotyping) characteristics. The phenotypic (biotypes, proteolytic and lipolytic profiles) and genotypic (16S-23 S PCR ribotyping) characteristics were used to conduct phylogenetic analysis. Ps. fluorescens belonged to 28 API 20 NE biotypes and 14 …
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Phenotypic and genotypic characterisation of Clostridium Difficile
… in this study, and the gold standard method, PCR ribotyping. The discriminatory power of both methods was compared and the similarity between the different isolates also analysed. Associations between the phenotypic and genotypic characteristics and the recovery location of the isolate were then …
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Prevalence and control of Clostridium difficile in patients with cystic fibrosis
… toxin being detected in 31.66% of patients. Ribotyping of the C. difficile isolates revealed 16 distinct ribotypes, including the hyper virulent RT078. All isolates were sensitive to both Vancomycin and Metronidazole. The effect of CF and its treatment on the gut microbiota of CF patients was …
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Pandemic <em>Vibrio parahaemolyticus</em>: Defining Strains Using Molecular Typing and a Growth Advantage at Lower Temperatures
… Molecular typing techniques; automated ribotyping, pandemic specific PCR and multilocus sequence typing (MLST), were compared to determine the best method for pandemic strain determination. MLST was the best method because it was the most informative and accurate. Furthermore, nine …
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An evaluation of DNA fingerprinting methods for subtyping Salmonella
… to 22 serotypes were fingerprinted by 16S RFLP ribotyping, by rep-PCR, using ERIC (enterobacterial repetitive intergenic consensus) primers, and by AP PCR. Ribotypes were shared by isolates from different serotypes. ERIC PCR and one AP PCR primer produced fingerprints that discriminated among …
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Agricultural Contaminant Source and Transport in a Karst Groundwater Basin
… water and groundwater within the basin. A ribotyping project that was done in conjunction with the thesis work showed the source of bacteria to be from cattle and humans. Nitrogen isotope analyses indicated that the source of nitrates was from ammonium fertilizers and from septic system …
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A clinical and molecular analysis of Clostridium difficile strains isolated from Groote Schuur Hospital
… sensitive in the local clinical setting. PCR ribotyping identified all the A-B+ strains as PCR ribotype 017, which was the prevalent strain type (47%). Genotyping based on the tcdC gene and MLVA both grouped the ribotype 017 strains in a single clade. The antimicrobial susceptibility of all …
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Diversity of the butyrate-producing microflora of the human gut.
… of the isolates to species level by ribotyping was possible only for two species, Eubacterium ventriosum and Fusobacterium prausnitzii. Selected isolates were grouped by phylogenetic analysis after determination of their 16S rDNA sequences. Most of the butyrate-producing isolates fell …
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Longitudinal study of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in a small and very small fresh meat processing plant environment
… isolates by molecular subtyping (e.g. PFGE, ribotyping) was performed to gain insight in contamination transmission within the facilities. S. enterica was isolated from 15 (4.5%) and 8 (2.4%) samples from Plant 1 and Plant 2, respectively. Characterization by PFGE using XbaI generated 6 …