Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Riboswitches."”.
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Structure and molecular recognition in riboswitches
Riboswitches are cis-acting gene regulatory RNAs, which function without involvement of proteins. They have been implicated as drug targets and are attractive systems for the study of RNA-ligand binding and RNA folding. The purine riboswitch was used as a model system for RNA-ligand docking. …
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Knockout of the trans-acting riboswitches in Listeria monocytogenes to disrupt downstream virulence factors
<p>Listeria Monocytogenes (L. monocytogenes) is a ubiquitous bacterium that can be found in both soil and water. This opportunistic intercellular pathogen is often food-borne and can lead to serious illness or death of those that are immunocompromised. Current medical research into the practical …
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Molecular biology of B vitamin metabolism genes and their regulation in Phaeodactylum tricornutum
… to exogenous thiamine. In many cases this is via riboswitches, sequences in mRNA that fold into a tertiary structure (aptamer) able to specifically recognise a ligand and mediate a change of genetic expression in response. In diatoms, marine algae responsible for ~20 % of net primary productivity, …
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DLRNA-BERTa: A transformer approach for RNA-drug interaction prediction
… RNA classes: aptamers, repeats, ribosomal RNAs, riboswitches, miRNAs and viral RNAs. A model was built for each RNA type, with a seventh general-purpose model combining all the data. We use a cross-attention layer and a linear layer computation to allow for interpretation of each token’s …
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CHARACTERIZATION OF A DUAL FUNCTIONING SAM-I RIBOSWITCH AND TRANS ACTING SMALL RNA IN DESULFOVIBRIO VULGARIS HILDENBOROUGH
… over the last decade. Both small RNAs and riboswitches have been shown in other bacteria to play key roles in sensing cellular conditions and eliciting the appropriate responses. A previous study in this laboratory identified several small regulatory RNAs, but their biological roles have …
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De novo designs of programmable toehold-mediated transcriptional attenuators
… genetic switches, such as riboregulators and riboswitches, are frequently utilized as translational and transcriptional regulatory elements to construct a diverse array of biological devices. RNA-only genetic circuitry provides several advantages over other types of genetic circuitry. While …
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An investigation into nucleic acid binding compounds
… use as drug targets. In achieveing this two riboswitches have been selected to be investigated: the thiamine pyrophosphate riboswitch and the PreQ1 riboswitch. The suitability of each of these riboswitches as drug targets has been explored; however, in the case of the thiamine pyrophosphate …
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Redefining the Coordination of Gene Expression Machineries in Bacillus subtilis
… helps to explain the increased use of riboswitches and RNA binding proteins in regulating transcription attenuation. In addition, I show that runaway transcription necessitates a more limited role for Rho-dependent transcription termination in B. subtilis, whereby Rho activity is …
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Preparation, Characterization, and Delivery of Antibodies Binding to a Model Oncogenic RNA, Human Initiator tRNA
… antibodies have been raised against microbial riboswitches and other structured RNAs of single-celled organisms, and only one human structured RNA to the best of our knowledge. However, no one has yet to create a synthetic antibody capable of behaving as a therapeutic against a structured RNA. …
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Mathematical frameworks for quantitative network analysis
… structures arise naturally in the study of RNA riboswitches - molecules that have an MFE binary structural degeneracy. We prove that this loop nerve complex has a euclidean 3-space embedding characterized solely by H2(R), its second homology group. We show that this group is the only non-trivial …
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Single-Molecule Studies Of Local And Global Nucleic-Acid Dynamics
… (c-di-GMP) riboswitch aptamer domain as a model. Riboswitches are examples of gene-regulating elements that normally act in cis to their host mRNAs and are hence located within their 5' untranslated regions. <i>in vivo,</i> the intracellular physiology, which is highly crowded, dictates the …
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Characterization of an Orphan Riboswitch: Identification of a Metal-Sensing Regulatory RNA
Riboswitches are RNA-based genetic control elements found in untranslated regions of the mRNA transcript that they regulate. These RNA motifs are highly structured and bind metabolites to elicit control of gene expression. Typically, the metabolite sensed by these RNAs is a component of the …
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Identification and characterization of thiamine biosynthesis, transport, and regulation elements among bacteroides species
… our attention to the conserved regulatory TPP riboswitches preceding thiamine biosynthesis and transport genes in B. thetaiotaomicron, B. uniformis, and B. vulgatus utilizing transcriptional and translational reporter assays. These assays have shown a clear regulatory hierarchy between thiamine …
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Methods of cellular integration and techniques for improving encapsulated cell viability for the purpose of developing living materials and cell based bio-sensing materials
<p>Living materials offer the potential for detection-reporter systems based on living cells that are genetically tailored to sense target analytes with high levels of specificity and accuracy. A biotic or living material would allow the development of simple, hand held devices that could be …