Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"Ribosome display"”.
-
A Pseudoknot Improves Selection Efficiency in Ribosome Display
The size and diversity of ribosome display libraries depends upon stability of the complex formed between the ribosome, mRNA and translated protein. To investigate if mRNA secondary structure improves stability of the complex, I tested a pseudoknot, originating from the genomic RNA of infectious …
-
In vitro selection and characterisation of human anti-HIV-1 antibody fragments
… human anti-HIV-1 antibodies in vitro by using ribosome display and selection from DNA libraries of HIV-1 patients. Two single-chain antibody libraries (M325 and K530) were constructed from two HIV-1 long-term non-progressors, whose sera showed cross-neutralising activities against various HIV-1 …
-
Molecular intervention in Pierce's Disease
… MopB protein. In vitro combinatorial antibody ribosome display libraries were assembled from immunoglobulin transcripts rescued from the spleens of mice immunized with heat-killed X. fastidiosa. The libraries were used in a single round of selection against the outer-membrane protein MopB, …
-
Novel Diagnostics and Therapeutic Approaches for Supratentorial Ependymoma
… fusion epitope were selected through phage-display and tested in immunoblot and immunohistochemistry assays before lead immunoglobulins were subjected to affinity maturation via ribosome display. Affinity-matured candidates were assessed for their diagnostic potential in immunoblot analysis …
-
Molecular display of synthetic oligonucleotide libraries and their analysis with high throughput DNA sequencing
… variable fragment (scFv) framework for molecular display. Our novel CDR sequence design utilized a hidden Markov model (HMM) that was trained on all antibody-antigen co-crystal complexes present in the Protein Data Bank. The resultant ~10¹² member library has been produced in ribosome display …