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Showing 1 to 20 of 54 for “"Ribosomally synthesized"”.
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Discovery and characterization of ribosomally synthesized and post-translationally modified peptide natural products
… rise in antibiotic- resistant pathogens. Ribosomally synthesized and post-translationally modified peptide (RiPP) natural products are genetically encoded peptide-derived secondary metabolites whose structures can be predicted based on gene sequence and identity of tailoring enzymes within …
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On the biosynthesis and discovery of ribosomally synthesized and post-translationally modified peptides
… microcins (TOMMs) comprise a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) that all contain thiazole and oxazole heterocycles derived from cysteine, serine, and threonine residues, respectively. The thiazole/oxazole heterocycle is installed over two …
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Biosynthesis and mode of action of ribosomally synthesized and post-translationally modified antimicrobial peptides
… acquired in recent years has revealed that ribosomally synthesized and post-translationally modified natural products are much more widespread than originally anticipated. Identified in nearly all forms of life, these natural products display incredible structural diversity and possess a …
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Characterization of Cyclopropyl Synthases Involved in the Maturation of Ribosomally Synthesized and Posttranslationally Modified Peptides
<p>Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a large class of natural products with significant human health implications. RiPPs are synthesized from a genetically encoded precursor peptide that undergoes significant modifications by maturing enzymes, or …
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Development of a synthetic biology platform to discover novel ribosomally synthesized and post-translationally modified peptides
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2026-12-01
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Biochemical characterization of enzymes involved in the biosynthesis of the thiopeptide thiomuracin
… modes of action. Thiopeptides are a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) that have outstanding biological profile, including high potency against a variety of antibiotic resistant pathogenic strains. Their new modes of action have attracted many …
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Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes
… cases are those falling under the class of ribosomally synthesized and post-translationally modified peptides (RiPPs), with a particular emphasis on the thiopeptide subclass of RiPPs. Additionally, characterization of an off-loading enzyme involved in hybrid polyketide/non-ribosomal peptide …
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Structural and Biochemical Studies of Nisin *Biosynthesis and Autoimmunity
… bacteria Lactococcus lactis spp. It is first ribosomally synthesized as an inactive precursor which is then post-translationally modified into its mature biologically active form. Described herein is a two-part study investigating (1) the structural basis of how the characteristic nisin …
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Development Towards an Ethylene Sensing Artificial Metalloenzyme
… biotinylated N-heterocyclic carbenes (NHCs) were synthesized, which can be appended onto the ruthenium probe. Efforts towards the expression and purification of the protein scaffold are also here reported.</p> <p>Mycofactocin (MFT) is a ribosomally synthesized and post-translationally modified …
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Discovery and structural characterization of lanthipeptides from soil and rumen bacteria
As members of the ribosomally synthesized and post-translationally modified peptide natural products (RiPPs), lanthipeptides possess myriad structural diversity and potential for discovery using genome-guided approaches. Biological activities of interest embedded within lanthipeptide structures …
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Development of lanthipeptide engineering strategies
… compounds. The lanthipeptides, members of the ribosomally synthesized and post-translationally modified peptide class of natural products, have potential applications ranging from clinical imaging to cystic fibrosis treatment. Additionally, many lanthipeptides possess strong antimicrobial …
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Biosynthesis and engineering of lanthipeptide natural products for novel applications
… chemical scaffolds and biological activities. Ribosomally synthesized and post-translationally modified peptides (RiPPs) have recently been recognized as a major class of natural products as a result of the genome sequencing efforts. The post-translational modifications endow them with diverse …
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Functional and mechanistic elucidation of peptide backbone thioamidation
… are found in a class of natural products named ribosomally synthesized and post-translationally modified peptides (RiPPs) and are critical for their biological functions. In addition to RiPPs, thioamide has also been observed as a post-translational modification in two essential protein …
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Investigation and application of substrate tolerant thioether forming enzymes
… them lanthipeptides are one of the most studied ribosomally synthesized and post-translationally modified peptides (RiPPs). In general, a precursor peptide with an N-terminal leader peptide and a C-terminal core peptide is ribosomally synthesized. The core peptides undergoes a series of …
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Sensitivity of Radical SAM Enzyme MftC to Molecular Oxygen
… step in the production of mycofactocin. It is a ribosomally synthesized post-translationally modified (RiPP) redox cofactor shown to be essential for the survival of bacteria in the <em>Mycobacterium</em> genus in the presence of cholesterol as a carbon source and for the sequestration of …
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Revealing the molecular details of azoline formation in ribosomal natural product biosynthesis
… (TOMMs) are a recently grouped class of ribosomally synthesized and posttranslationally modified peptides. Encoded by many bacteria and archaea, these natural products occupy a large chemical and functional space and are linked by the presence of the eponymous azole moieties. Previous …
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Mechanistic Studies of Lantibiotic Biosynthetic Enzymes and Discovery of Novel Lanthionine-Containing Peptides
Lantibiotics are ribosomally synthesized antimicrobial peptides containing unique thioether crosslinks, lanthionines and methyllanthionines. These motifs are introduced posttranslationally in a two-step process, which involves the dehydration of serines and threonines into dehydroalanines and …
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Biosynthesis of the antimicrobial peptide epilancin 15X
… are polycyclic antimicrobial peptides that are ribosomally synthesized and posttranslationally modified to their biologically active forms. The recently discovered lantibiotic epilancin 15X produced by Staphylococcus epidermidis 15X154 is active against several pathogenic bacteria, including …
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Expanding the structural diversity of the RiPP class of natural products
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a diverse and rapidly expanding class of natural products. Despite starting out as linear chains of amino acids, RiPP natural products acquire structural diversity ranging from small molecules like microcin C7 to 48-mer …
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Design of Post-Translationally Modified Peptides by Combining Enzymes from Diverse Pathways
Over the past decade, ribosomally-synthesized and post-translationally modified peptides (RiPPs) have emerged as both therapeutically-relevant and engineerable, two traits previously unobserved together in a natural product class. Their biosynthesis is modular: a precursor peptide recruits enzymes …
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