Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Ribosomal frameshift"”.
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Evaluating the Conservation of RNA Packaging Enhancer Elements Across Lentiviruses
… al. discovered that the region overlapping the Ribosomal Frameshift Signal (RFS) acted to enhance RNA packaging 50 fold and was thus named the genomic RNA packaging enhancer element (GRPE). To determine if the GRPE was conserved across lentiviruses, using the same approach as Chamanian et al., …
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Messenger RNA Destabilization by -1 Programmed Ribosomal Frameshifting
… previous studies have identified programmed -1 ribosomal frameshifting (-1 PRF) signals in eukaryotic genomic sequences, and suggested a role in mRNA stability. This work improves and extends the computational methods used to search for potential -1 PRF signals. It continues to examine four …
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Recoding and reassignment in protists
… profiling, I determined over 1,700 cases of ribosomal frameshifting (22% of genes analysed) in E. crassus. I identified 47 codons upstream of a stop signal which directs the ribosome to either the +1 or +2 reading frames. Termination only occurs in the context of the poly-A tail. In addition …
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Characterization of Human T-Cell Leukemia Virus 1 Reverse Transcriptase
… as well as information about the location of the ribosomal frameshift, the location of the putative coding sequence for the enzyme has been identified. This information and PCR amplification was used to construct a clone, which spans a region of the <em>pro-pol</em>junction of HTLV-1, for …
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Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression
… cells. The PRRSV genome contains two programmed ribosomal frameshift (PRF) signals, at which conserved elements promote the backwards slippage of the ribosome by one or two nucleotides. PRRSV encodes a canonical −1 PRF site, at which frameshifting is directed by a downstream RNA pseudoknot …
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A Novel HIV-1 Genomic RNA Packaging Element and its Role in Interplay between RNAPackaging and Gag-Pol Ribosomal Frameshifting
… translation and genome packaging.The ribosomal frameshifting step of translation is critical for efficient synthesis of Gag and Gag-pol proteins through shifting of ribosome in -1 nt direction at the ribosomal frameshift signal (RFS). After identification of a Genomic RNA Packaging …
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Multi-color visualization and quantification of single RNA translation and HIV-1 programmed ribosomal frameshifting in living cells
… imaging modality was used to investigate ribosomal frameshifting during translation. Frameshifts occur through a ribosomal +1(-2) or -1 (+2) nucleotide(s) "slip" into another frame and are implicated in both human disease and viral infections. While previous work has uncovered many …
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Structure and Function Studies of the Orsay Virus δ Protein
… either as a free δ or a CP-δ fusion protein by ribosomal frameshift, but their structure and function were mostly unknown. To get a better understanding of the infection mechanism of this novel virus, I performed both structural and functional studies of the free δ protein as well as the CP-δ …
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Super-Resolution Force Spectroscopy for Revealing Sub-nucleotide Motion in Ribosomal Frameshifting and DNA Methylation
Programmed ribosomal frameshifting plays an important role for many viruses in their protein synthesis, with well-known cases including HIV and SARS-CoV-2. Aiming for an approach to defend against such viruses, techniques including X-ray crystallography, cryo-electron microscopy (Cryo-EM) and …
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Novel expression and production of Foot-and-mouth disease virus vaccine candidates in Nicotiana benthamiana
… by introducing mutations in the 3C gene or a ribosomal frameshift in the gene sequence which subsequently reduce its deleterious effect. Our laboratory has previously shown that similar FMDV VLPs can be assembled by the expression of FMDV P1-2A (referred to as oP1-2A, in this study), in the …