Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Ribonucleoproteins"”.
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Distinct Examples of Immunologic Analysis in Molecular and Cellular Biology: Small Nuclear Ribonucleoproteins and the T Cell Receptor
<p>Since the discovery (Tan and Kunkel, 1966; Mattioli and Peichlin, 1971; Sharp, et al., 1972) of human SLE autoantibodies directed against RNA-protein complexes in eucaryotic nuclei, a great deal of interest has been generated regarding both the relationships of antibody specificities to disease …
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Localization of Small-Nuclear-Rna Genes of Drosophila Melanogaster on Polytene Chromosomes
… RNAs associate with hnRNA and small nuclear ribonucleoproteins (snRNPs) act as antigens that are precipitated by antibodies from patients with lupus erythematosus, an auto-immune rheumatic disease, have sparked new interest in these molecules. snRNAs have been found to be highly conserved in …
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Structure and Dynamic Studies of the Nuclear Pore Complex at the Single-Molecule Level
… (NSOM) are utilized to specifically locate vault ribonucleoproteins to NPCs. This interaction, along with several other results, strongly suggests that vaults represent the central mass of NPCs. A single-molecule transport assay was also developed in order to record the translocation of individual …
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Investigation of Vesicular Stomatitis Virus Interaction with Host Cell Proteins and Associated mRNA Structures
… of RNA-binding proteins, heterogeneous nuclear ribonucleoproteins (hnRNPs), relocalized from the nucleus to the cytoplasm during infection with VSV. We performed co-immunoprecipitation and RNA-seq experiments to identify and quantify the RNAs that interacted with hnRNP A1, hnRNP C1/C2, and hnRNP …
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Molecular analysis of peasnRNA variants
… in regions implicated in the binding of small ribonucleoproteins and may result in the formation of unique snRNPs during development.
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Functional characterization of the RNA binding protein RALY
… RNA metabolism. The heterogeneous nuclear ribonucleoproteins (hnRNPs) belong to big family of RBPs involved in many aspects of RNA metabolism including RNA stability, intracellular transport and translation. More recently, RALY, a RNA-binding protein associated with the lethal yellow …
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Biochemical and Functional Characterization of Novel RNA-binding Proteins Interacting with SMN in Motor Neuron-derived Cells
… assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), it remains a crucial question in the field to gain a better understanding of what specific/distinct function(s) SMN might have in motor neurons. We have identified novel interactions between SMN and two RNA-binding …
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Development of Specific Inhibitors that Prevent hnRNPA1-Mediated Therapy Resistance in Small Cell Lung Cancer.
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a diverse family of RNA-binding proteins (RBPs) that are involved in many steps in gene regulation. The hnRNP family consist of 20 members which are expressed in humans (A1 to U). The first member identified being hnRNP A1; this is a …
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HUMAN GENETIC VARIANTS IN ESSENTIAL SPLICING FACTORS AND THEIR IMPACT ON IMMUNE PATHOLOGY
… which is composed of five small nuclear ribonucleoproteins (snRNPs) and hundreds of proteins that are generally referred to as splicing factors (SFs). Here we present two unique cases in which genetic variations in two essential SFs, DExD-box polypeptide 39B (DDX39B) and U2 small nuclear …
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The Effect of Stress Induced Premature Senescence on the Expression of Heterogeneous RibonucleoIeoprotein
<p>The role of heterogeneous nuclear ribonucleoproteins (hnRNP) in cellular senescence is yet to be defined. Cellular senescence is a terminal growth arrest in somatic cells. It is thought to be the consequence of telomeric shortening that acts as a DNA damage signal. Conversely, cells induced into …
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Efficient Methods for Ex Vivo Generation of Gene-edited Neutrophils
… Stable modification of progenitors by Cas9 ribonucleoproteins or lentiviral vectors followed by granulocytic differentiation yields uniform populations of gene edited neutrophils in an activation-free manner, allowing the interrogation of individual genes in the neutrophil context. I use …
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Role of hnRNP A1 in the Regulation of HDM2 Gene Expression
… expressed member of hnRNPs (Heterogenous Nuclear Ribonucleoproteins) family of proteins that play multiple roles in gene expression by participating in major steps in the processing of nascent RNA transcripts 1. It is involved in mRNA biogenesis mechanisms such as the transcription, splicing, …
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CHARACTERIZATION OF INFLUENZA NUCLEOPROTEIN BODY DOMAIN AS ANTIVIRAL TARGET
… dependent RNA polymerase (RdRP) to form viral ribonucleoproteins (vRNPs) responsible for RNA synthesis. NP is a structural component of the vRNP but also interacts with both viral and host factors to regulate viral RNA expression. NP is conserved among influenza A isolates, making NP …
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Ruolo della TDP-43 nella formazione dei granuli da stress nella Sclerosi Laterale Amiotrofica
… structures composed of non-translating messenger ribonucleoproteins (mRNPs) that rapidly aggregate in cells exposed to adverse environmental conditions. Stress granules function in part to triage RNA and sequester transcripts not needed for coping with the stress. We evaluated stress granule …
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Identification of determinants of sensitivity to duocarmycin analogues: A class of potent anti-cancer drugs
… line was established by using dual CRISPR-Cas9 ribonucleoproteins. The effect of ALDH1A1 knockout on the cytotoxicity of duocarmycin analogues was tested by anti-proliferative and anti-clonogenic assays. The results showed that ALDH1A1 does not play a key role in the MOA of these duocarmycin …
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Structural studies of spliceosome assembly and catalysis
… mRNA (pre-mRNA) from its component small nuclear ribonucleoproteins (snRNPs) and protein factors. A series of conformational and compositional changes produces the catalytically active spliceosome, in which the snRNA components of the snRNPs mediate intron recognition and catalysis of splicing. …
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Development of Oligonucleotide-directed proximity-interactome MAPping (O-MAP), for characterizing RNA-protein interactions and higher order subnuclear architecture in situ
… in disease. RNA-protein complexes, termed ribonucleoproteins (RNPs), can also serve as structural scaffolds for reorganizing local subcellular environments and assembling subnuclear bodies. A classic example of RNA-scaffolded structures is the nucleolus, a subnuclear body that serves as the …
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Defining the Cellular and Molecular Basis of Spondyloepiphyseal dysplasia tarda
… inhibition by sequestering free mRNAs and ribonucleoproteins in cytosolic aggregates named stress granules (SGs). I demonstrated that SGs control secretion by recruiting key components of ER-to- Golgi trafficking and thus inhibit anterograde transport. This suggested that the ISR regulates …
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Development of genome editing in potato and tobacco
… methods, such as transfection of CRISPR/Cas9 ribonucleoproteins (RNPs) in protoplasts followed by plant regeneration (Chen et al., 2019). The non-transgenic approach is preferred in many cases as it avoids the introduction of trans DNA, helping to ease regulatory requirements in some …
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