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Showing 1 to 20 of 20 for “"Ribonuclease A"”.
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Interaction of Cu(II) ion with ribonuclease A in the native and unfolded states
<p>The binding of Cu(II) ion to ribonuclease A at various sodium perchlorate concentrations was studied as a function of Cu(II) ion concentration and pH using a Cu(II) ion electrode. Spectrophotometric studies of the same systems were also carried out. The copper binding studies were carried out at …
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Quantenmechanische Berechnung der CD-Spektren von Cyclohexandionderivaten, Lactamen, Ribonuclease A sowie von Androstan-Bisporphyrinen
… derivatives, bicyclic lactams, the protein Ribonuclease A and androstan-bisporphyrins. The objective for the smaller molecules was to predict the absolute configuration of the compounds by comparing the calculated and experimental CD-spectra. The CD-spectrum of the cyclohexandione …
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Information Content in Ribonuclease A: An Investigation of Protein Structure and Identification of New Qsars
… model was developed for this quantification. Ribonuclease A (RNase A) was used as a model system of investigation.</p> <p>In solution, molecules are subjected to numerous collisions powered by the thermal energy of Brownian motion. It is upon each Brownian collision that structural information …
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NMR study of the unfolding of ribonuclease A, and dynamical studies of liquids in confined geometries
The cold, heat, and pressure unfolding of RNase A has been studied by 1D and 2D $\sp1$H NMR spectroscopy, spin-lattice relaxation time measurements, and the pressure-jump hydrogen-exchange method. It was found that the pressure denatured states of RNase A display some characteristics of a molten …
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Substitution of the Anticodon Loop of Yeast Tyrosine Transfer Ribonucleic Acid (Rna)
… of tRNA('Tyr) from partial digestions with ribonuclease A or T(,1) were paired to create a gap in the anticodon loop. An oligonucleotide was inserted into the gap using RNA ligase, polynucleotide kinase, and Pset 1 polynucleotide kinase. The rate of aminoacylation of anticodon loop …
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Thermal unfolding dynamics of proteins probed by nonlinear infrared spectroscopy
… used to measure equilibrium thermal unfolding of ribonuclease A and ubiquitin. For transient unfolding studies, the temperature of the solution is rapidly raised by a nanosecond temperature jump (T-jump) laser, which is followed by probing structural changes of proteins with dispersed vibrational …
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Biospeciation, Interaction with Biomolecules and Mechanism of Action of Potential Cu and V Drugs
… as myoglobin, ubiquitin, lysozyme, cytochrome c, ribonuclease A, and serum albumin were analyzed by electrospray ionization mass spectrometry (ESI-MS), electron paramagnetic resonance (EPR) spectroscopy, quantum-mechanical (QM) calculations, and molecular docking. These studies identified specific …
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Substrate specificity and properties of heat-stable proteases from Pseudomonas fluorescens
… generated from the hydrolysis of oxidized ribonuclease A by proteases of known specificity (trypsin, chymotrypsin, Staphylococcus aureus protease) and the pseudomonal proteases were separated by RP-HPLC. The proteases of known specificity generated a low number of peptides in relatively …
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Redox active tyrosine residues in biomimetic beta hairpins
… discovery of autonomously folding fragments of ribonuclease A and tendamistat pioneered the use of biomimetic peptides for determining how the polypeptide sequence stabilizes formation of alpha helices and beta hairpins in aqueous and organic solutions. A set of rules for constructing stable …
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Microfluidic platforms for protein crystallization and in situ X-ray analysis
… the soluble proteins lysozyme, thaumatin, ribonuclease A, bacterial lyase and bacterial defluorinase on-chip and performing in situ X-ray crystallographic analysis."
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Activated Phosphate Reagents for the Synthesis of Functionalized Oligophosphates
… for inhibition of the enzymatic activity of ribonuclease A. We then solved X-ray co-crystal structures of the highest affinity nucleotide binders to rationalize the increased binding affinity of longer nucleotides. The anion [P4O₁₁] ²⁻, employed as its bis(triphenylphosphine)iminium (PPN) …
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MEMS-based temperature-dependent characterization of biomolecular interactions
… of 18-Crown-6 and barium chloride, as well as ribonuclease A and cytidine 2'-monophosphate, in a 1-µL volume with low concentrations (ca. 2 mM). Thermodynamic properties, including the stoichiometry, equilibrium binding constant, and enthalpy change, are also determined.
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Biosynthesis of polyhydroxyalkanoates and their medical applications
… treatment encountered by the model protein Ribonuclease A (RNase A) during microencapsulation in P(3HB) microspheres were investigated. From the results, structural integrity of RNase A was maintained. A gradual decrease in the enzyme activity of RNase A was observed over a period of time.