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Showing 1 to 9 of 9 for “"Rhodamine 123"”.
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P-glycoprotein and membrane permeability as determinants of xenobiotic bioavailability and bioaccumulation
… biliary excretion of Pgp prototypic substrate Rhodamine-123 in isolated perfused livers. Further, dieldrin (20µM) reduced movement of 3H-tetracycline (8.8nM) into bile (55%) to a greater extent than Pgp prototypic competitive inhibitor verapamil. In contrast to inhibitory effects in situ, a …
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AVALIAÇÃO DA ATIVIDADE ANTILEISHMANIA DA PLA2 Lys49 MjTX-I, ISOLADA DA PEÇONHA DE Bothrops moojeni
… in the assay (10 and 40 μg / mL) using the rhodamine 123 probe. MjTX-I interfered with the invasion capacity of the pre-treated promastigotes (10 and 40 μg / mL), decreasing the number of infected cells and the number of parasites per infected cell. Finally, the treatment of infected …
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Developing and Utilizing Gas-phase Laser Spectroscopy Techniques to Study the Effect of Desolvation on Molecules
… In Chapter 2, specific changes in the simple rhodamine scaffold, starting with rhodamine 123 are shown to induce changes in the dyes’ fluorescence excitation and emission spectral profiles in the gas phase. The possibility of these changes being indicative of the effect of solvent interactions …
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Advanced Fluorescence Lifetime Imaging and Spectroscopy Techniques for Biological Samples
… addition, spectroscopic studies of Nile red, Rhodamine 123, Pyrromethene 546 and BODIPY-C12 in methanol/glycerol mixtures were carried out to examine the relationship between the radiative rate constant and refractive index according to the Strickler-Berg equation and an advanced model by …
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Metabolic flux analysis and population heterogeneity in mammalian cell culture
… measured using the common mitochondrial stain, Rhodamine 123. The MMP sorted subpopulations were subjected to apoptosis inducers, and the apoptotic death was characterized both morphologically through the determination of apoptosis related chromatin condensation and also biochemically through …
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Electric field-assisted immobilization and alignment of biomolecules
… was demonstrated with the oxidation of dihydrorhodamine 123, a non-fluorescent precursor of the fluorescence dye rhodamine 123. Molecular alignment and immobilization - reversible and permanent - was achieved under the influence of inhomogeneous AC electric fields. For orientational …
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Evaluation of Stallion Frozen-Thawed Semen Using Conventional and Flow Cytometric Assays
… morphology, mitochondrial activity using Rhodamine 123 (R123), plasma membrane integrity using propidium iodide (PI) and ethidium monoazide (EMA), and chromatin structure using the sperm chromatin structure assay (SCSA). Data was recorded as percentages for all but the SCSA for both …
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Investigation of surfactant-drug pharmacokinetic interactions
… apparatus. The bidirectional transport of Rhodamine 123 (Rho123), a substrate of the P-glycoprotein (P-gp) efflux transporter with fluorescent properties, was measured in the presence of five surfactants and a bile salt, namely Brij® 58, Tween® 20, Span® 20, Cremophor® CO40 and sodium …
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Study of the etiology of neurolathyrism, a human neurodegenerative disease with nutritional causes
… membrane potential – visualized with Rhodamine-123 - was hyperpolarized in cells treated with L-β-ODAP. In primary motor neurons, grown on a glial cell feeder layer, the effect of acute exposure to L-β- ODAP or L-glutamate was studied while intracellular Ca2+ responses ([Ca2+]i) were …