Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"Rho GTPases"”.
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Rho GTPases in Neuronal Apoptosis and Neurodegeneration
<p>Several studies have identified Rho family GTPases (i.e. Rho, Rac, Cdc42) as mediators of diverse critical cellular processes, such as actin cytoskeleton remodeling, gene transcription, cell-cell adhesion, and cell cycle progression. However, more recent data highlight an essential role for Rho …
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Characterization of Vibrio VopS, an AMPylator of Rho GTPases
… Our studies have found that VopS inhibits Rho GTPase signaling during infection by directly modifying Rho, Rac, and Cdc42, preventing their interaction with downstream effectors. These observations reveal a unique activity for VopS, which targets a pathway that is critical in the cellular …
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The generation and characterization of transgenic mice expressing modifiers of Rho GTPases
… their target. Recent studies have suggested that Rho family GTP-binding proteins are important components of the signalling pathways that link the reception of extracellular cues to the cytoskeleton. Rho family GTP-binding proteins regulate many different aspects of the actin cytoskeleton in a …
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Dissecting the Function of FARP1 and Rho GTPases in Semaphorin-Plexin Signaling: Structural Perspective
… screening with yeast two-hybrid identified a RhoGTPase, Rif, as a novel interacting protein for FARP1. To investigate how Rif/FARP1 interaction is involved in Plexin signaling, I determined the crystal structure of Rif-bound FARP1. This complex structure explains the functional roles that …
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Molecular control of endothelial lumen formation by Rho GTPases in three dimensional collagen matrices
… OF MISSOURI-COLUMBIA AT AUTHOR'S REQUEST.] Rho GTPases regulate a diverse spectrum of cellular functions involved in vascular morphogenesis. Here, we show that Cdc42 and Rac1 play a key role in endothelial cell (EC) lumen and tube formation in 3D collagen matrices, and that their regulation …
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Rho GTPases and their regulators in cell polarity of the filamentous ascomycete Neurospora crassa
Rho-GTPasen sind kleine G-Proteine der Ras-Superfamilie, die als molekulare Schalter wirken: Im GTP-gebundenen Zustand aktivieren sie eine Vielzahl von Effektorproteinen, wohingegen sie durch die Hydrolyse des GTPs wieder inaktiviert werden. Rho-GTPasen nehmen eine Schlüsselstellung in zahlreichen …
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Role of Rap and Rho GTPases in T-acute lymphoblastic leukaemia cell adhesion and migration
… of T-ALL cells in the tissues. Rap and Rho family GTPases play key roles in T cell adhesion and migration and are often involved in cancer progression. Most of these proteins are post-translationally isoprenylated to facilitate their anchorage to membranes, where they function to …
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Reconstitution Of Rho Gtpase Interactions At The Membrane
… signal with a robust morphological change. The Rho Family of GTPases play critically important roles in mediating these cellular processes. However, in order for the Rho GTPases to help regulate these processes in a tightly coordinated spatial and temporal manner, they need to be properly …
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Regulation of RhoA Activation and Actin Reorganization by Diacylglycerol Kinase
Rho GTPases are critical regulators of actin cytoskeletal dynamics. The three most well characterized Rho GTPases, Rac1, RhoA and Cdc42 share a common inhibitor, RhoGDI. It is only recently becoming clear how upstream signals cause the selective release of individual Rho GTPases from RhoGDI. For …
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Characterisation of the expression profile and endothelial function of Rho GTPase RhoJ
Rho GTPases are molecular switches that regulate many aspects of cell physiology. A number of Rho GTPases are essential for the formation of new vessels from pre-existing ones, a process known as angiogenesis. RhoJ/TCL belongs to the Cdc42 subfamily of Rho GTPases. Previous bioinformatic and …
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Control of sex myoblast migration in C. elegans
… Similar to vertebrate systems, C. elegans uses Rho family small GTPases to regulate the engine of cell motility, the actin cytoskeleton, in response to guidance cues. The differential utilizations of Rho GTPases in distinct processes in vivo remain a central question in the study of Rho GTPases. …
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Investigating the role of zoledronate for inhibition of osteosarcoma metastasis
… cytoskeletal rearrangement largely controlled by Rho GTPase proteins (RhoA, RhoC, Rac-1 and Cdc42) and effector pathways (Rho-kinase; ROCK). Given their pivotal role in directional cell migration, perturbing the function of Rho GTPases is considered a viable anti-metastasis strategy. Disruption of …
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Bioprobes and Genetics Reveal the Signal Integration That Initiates Dendrites in a Neuron in Vivo
… intrinsic signaling molecules. Monomeric Rho GTPases are capable of mediating diverse transmembrane signal pathways. However, the means to monitor and dissect molecular signaling within a subcellular compartment are limited, and the role of Rho GTPases and their partners remains unresolved …
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The Role of Rac and Filamin A in Osteoclast Function and Osteoclast Heterogeneity
… dynamic actin cytoskeleton that is regulated by Rho GTPases. Rho GTPases function as molecular switches that regulate the actin cytoskeleton in osteoclasts and many other cell types. A Rac GTPase validation experiment was used to prove that osteoclast function is best characterized through …
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RhoA and Rac1 prenylation: Effects on serine/threonine signaling for actin polymerization
RhoA and Rac1 are small guanosine triphosphates (GTPases) that regulate cytoskeletal rearrangements, cell polarity and axon guidance and signaling pathways. Alteration of the subcellular localization or activation of Rho GTPases is implicated in several neurological conditions. Rho GTPases are …
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Αvβ8 Integrin Interacts With Rhogdi1 to Regulate Rac1 and Cdc42 Activation and Drive Glioblastoma Cell Invasion
… cell invasion due to hyperactivation of the Rho GTPases Rac1 and Cdc42. β8 integrin associates with Rho GDP Dissociation Inhibitor 1 (RhoGDI1), an intracellular signaling effector that sequesters Rho GTPases in their inactive GDP-bound states. Silencing RhoGDI1 expression or uncoupling αvβ8 …
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Structural and Kinetic Characterization of Protein Ampylation by VopS Fic Domain
… threonine residue in the switch 1 region of Rho GTPases. The modified GTPases are no longer able to interact with downstream effectors due to steric hindrance by the covalently linked AMP moiety. Herein we analyze the structure of VopS and its evolutionarily conserved catalytic residues. We …
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Investigating the Functional Interaction between RhoGDI Family Proteins and Activated Cdc42 associated-kinase (ACK)
… yeast-2-hybrid screen in the lab identified RhoGDI-3 as an interacting partner for ACK. RhoGDI-3 is a member of RhoGDI family of proteins, which are negative regulators that maintains Rho-family GTPases in the inactive GDP-bound state and sequesters them in the cytosol. RhoGDIs have also been …
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Rho GTPase family members in establishment of polarity in C. elegans embryos
… by RNA-mediated interference (RNAi) of two Rho GTPases, CDC-42 and RHO-1. To examine how Rho GTPases are implemented in actin remodeling, it is important to analyze how their activity is controlled and how different activities affect polarity formation. The role of two putative Rho GTPase …
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Biophysical and computational insights into the stability of RhoB and its cancer-associated variants
RhoB is a small GTPase, part of the Rho family of the Ras superfamily of small G proteins, that act as cellular signalling switches. RhoB is involved in regulating the cytoskeleton, angiogenesis, cell death, endosome trafficking and DNA damage repair. Unlike other small GTPases such as K-Ras, RhoA …
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