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Showing 1 to 14 of 14 for “"Rex1"”.
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The P-Rex1 Regulator Norbin Suppressess Neutrophil-Dependent Antibacterial Immunity
P-Rex1 is a guanine-nucleotide exchange factor (GEF) that activates the small G protein Rac, thus regulating a wide range of physiological and pathophysiological responses. Neutrophils are leukocytes of the innate immune system in which P-Rex1 regulates several Rac-dependent responses, especially …
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The Rac-GEF P-Rex1 Regulates Agonist-Induced GPCR Trafficking
… a comprehensive insight into the role of P-Rex1 in GPCR trafficking. Upon generating a P-Rex1 knock-out PC12-S1PR1-GFP cell line, I used imaging and cell fractionation methods to show that endogenous P-Rex1 limits the agonist-induced internalisation of S1PR1 and quantified this effect. I …
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Upregulation of PIP3-dependent RAC Exchanger 1 (P-Rex1) Promotes Prostate Cancer Metastasis
… cascades. We investigated the role of P-Rex1, a novel specific GEF for Rac, in human prostate cancer metastasis. P-Rex1 expression (mRNA and/or protein) was almost undetectable in normal prostate epithelial cells (PrEC) and two nonmetastatic prostate cancer cell lines (LNCaP and …
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Identification of the Role of P-Rex1 and its Adaptor Functions in Neutrophils
P-Rex1 is a guanine-nucleotide exchange factor (Rac-GEF) for the small GTPase Rac, recognised for its involvement in neutrophil migration and ROS generation in response to GPCR activation—functions traditionally attributed to its Rac-GEF activity. Additionally, previous findings from our laboratory …
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Invesitagating the role of the guanine nucleotide exchange factor P-Rex1 in cell invasion
… form and function of the actin cytoskeleton. P-Rex1, a Rac guanine nucleotide exchange factor (GEF), was previously identified in this lab in a transcriptional screen for mediators of cell invasion. A number of GEFs have been shown to regulate the invasive phenotype of tumours and cancer cell …
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Function and Regulation of Phosphatidylinositol 3,4,5-Triphosphate-Dependent Rac Exchanger 1 (P-Rex1) in Cancer
… Rac exchange factor 1 (P-Rex1) promotes tumorigenesis and metastasis of various cancers, and thus could be a potential therapeutic target. The first objective of my study was to determine the molecular mechanism regulating P-Rex1 gene expression. We found that P-Rex1 …
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New roles of Rac-GEFs in Neutrophils and in Glucose Homeostasis
… crossed to mice deficient in the Rac-GEFs P-Rex1/Vav1, DOCK2 or Tiam1. I demonstrated that Rac activity is increased in RFC Tiam1–/– neutrophils adhering to integrin ligands, which may explain the increased adhesion. In contrast, RFC DOCK2–/– and RFC P-Rex1–/– Vav1–/– neutrophils had reduced …
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Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells
… SIGNALING MODULATES OCT4, NANOG,</p> <p>AND REX1 EXPRESSION AND INDUCES PRIORITIZED DIFFERENTIATION OF</p> <p>MURINE EMBRYONIC STEM CELLS</p> <p>by</p> <p>JILL SLATER</p> <p>MAY 2013</p> <p>Advisor: Daniel Rappolee, Ph.D.</p> <p>Major: Physiology</p> <p>Degree: Doctor of Philosophy</p> …
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Understanding the Dynamics of Embryonic Stem Cell Differentiation
… from pluripotency. Expression of the reporter Rex1-GFPd2, which is inactivated upon exit from naive pluripotency, was analyzed by quantitative long-term single-cell imaging over many generations. This technique allowed chemical, physical, and genealogical information to be recorded during the …
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Investigating mechanisms of genome folding by single-cell Hi-C
… from cells expressing the pluripotent marker Rex1 after 24 hour differentiation are similar to the shapes of chromosomes in ES cells; whereas chromosome shapes from cells both after 24 hour differentiation with low Rex1 expression and after 48 hour differentiation are even more varied.
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P-REX2 PH Domain Inhibition of PTEN Regulates Transformation, Insulin Signaling, and Glucose Homeostasis
… Furthermore, the PH domain of P-REX1 was not able to inhibit PTEN, and full length P-REX1 did not interact with PTEN, suggesting that there is a level of specificity involved in P-REX2 PH domain mediated phosphatase inhibition and binding. Overall, this thesis identifies P-REX2 as …
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The effects of magnetic field (MFs) on in vitro culture of human umbilical cord derived mesenchymal stem cells (hUC-MSCs)
… markers (OCT4, SOX2, NANOG, and REX1) in DE group than in the IE and NC groups as analyzed via RT-PCR. In conclusion, through MF exposure, small quantity of collected MSCs could be expanded rapidly in a short period of time. This finding may suggest MF as a new modality to expand …
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Expression Analysis and Stem Cell Engineering
… from three pluripotency genes: Oct3, Nanog, and Rex1 from a single blastocyst, containing 30 ICM cells. The polony method is sensitive, can be applied to most genes, and allows for a degree of multiplexing. Second, DNA methylation was explored as a tool to measure cell fate as ES cells are …
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Studying the direct effects of forces on embryonic stem cell behaviour
… as we showed using gene expression studies and a Rex1 reporter. To study how ES cells' susceptibility to mechanical signals depended on media condition, stretch duration and stretch type, we performed RNA sequencing and used gene ontology techniques to investigate the involvement of specific …