Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 36 for “"Reversed-phase HPLC"”.
-
Reversed-Phase HPLC Determination of Cholesterol in Food Items.
… in the body and cardiovascular disease. An HPLC method for quantitative determination of cholesterol in foods is presented. This involves a C-18 stationary phase using a 70:30 methanol: 2-propanol mobile phase with an UV detector set at 212 nm. The method showed linearity in the range 5.0 to …
-
Reversed-Phase HPLC Determination of Alliin in Diverse Varieties of Fresh Garlic and Commercial Garlic Products.
… research presents a simple, rapid, and precise HPLC method for alliin determination. It involves the use of 30:70% methanol: water and 0.05% sodium dodecylsulfate mobile phase composition, C<sub>18</sub> 5 μm disc column of size 3.9 x 150 μm, and detector set at 210 nm. The method showed good …
-
An investigation into the use of B-cyclodextrins as additives to effect enantiomeric separation by reversed phase HPLC.
… The ability of B-cyclodextrin (CD) as mobile phase additive in reversed phase HPLC to bring about chiral discrimination using acetonitrile/phosphate buffer and an octadecylsilica stationary phase has been investigated with methylphenobarbitone, ibuprofen, hexobarbitone and mandelic acid as …
-
Nutritional Studies on the Utilization of Winged Bean (Psophocarpus Tetragonolobus L. Dc) Seeds and Leaves (Carotenoids, Bean-Curd, Anti-Nutrients, Reversed Phase Hplc, Phytic Acid)
Protein, fat, mineral and anti-nutrient contents of seed flour of 12 cultivars of winged bean (Psophocarpus tetragonolobus L.DC) grown in Sri Lanka were determined. Protein and oil contents in dehulled seed flour ranged from 37.4-46.9 and 20.0-26.5% on dry weight basis, respectively. Of the …
-
Studies of atrial natriuretic peptide in the freshwater turtle Pseudemys scripta
… to purify further the active component using reversed-phase HPLC techniques and the radio-receptor assay resulted in the isolation and sequencing of two ANP receptor-binding peptides. These peptides were not homologous to ANP but rather were homologous to the muscle protein fragments porcine …
-
Design and Synthesis of a Novel Neuroprotective Brain-Targeting Amylin Receptor Antagonist
… targeting. R16 was synthesized using solid-phase peptide synthesis, purified and characterized by MALDI-TOF mass spectrometry and reversed-phase HPLC. The proteolytic stability of R16 was evaluated in human and mouse sera to determine the half-lives and proteolytically labile sites. The …
-
The Isolation and Evaluation of Biological Effects of the Glucosinolate Hydrolysis Product 1-Isothiocyanato-3-(methylsulfinyl)-Propane
… using gel filtration chromatography and reversed-phase HPLC.
-
Determination of Lactose by Reversed-Phase High Performance Liquid Chromatography.
… The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water …
-
Photoaffinity labeling the nucleotide sites of the sarcoplasmic reticulum Ca²⁺-ATPase
… as well as by anion exchange TLC and reversed phase HPLC. In the dark, the compounds were found to display most of the features of the parent TNP-nucleotides and interacted with the Ca²⁺-ATPase in a similar way. When activated by illumination, the probes were specifically incorporated …
-
QUB-1157: a bioactive peptide from the skin secretion of the Mexican leaf frog (Pachymedusa dacnicolor)
… techniques were used in this thesis. Solid phase peptide synthesis was used to synthesise replicates of the novel peptide, and reversed-phase HPLC and MALDI-TOF MS was applied to purify peptide and confirm the mass. According to the peptide’s sequence, this novel peptide has been classified …
-
Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… affinity chromatography then followed by reversed-phase-HPLC. Protein was quantified using bicinchoninic acid (BCA) assay to know the protein yield concentration. Proteolytic cleavage of enterokinase site was done and untagged Ts6 was reached. Electrophysiological recordings were done on …
-
High-performance liquid chromatographic methods for quantitative assessment of degradation products and extractives in pretreated lignocellulose.
… for accurate quantitative determinations. A reversed-phase HPLC method with UV detection is developed for simultaneous separation and quantitation of organic acids and neutral degradation products present in the corn stover hydrolysate. On the other hand, inorganic ions and some organic …
-
The ring-opening polymerization of cyclosiloxanes in the presence of bis ([alpha], [omega]-aminopropyl)-1,3-tetramethyldisiloxane
… catalysts has been investigated. The use of reversed-phase high performance liquid chromatography (HPLC) and capillary gas chromatography (GC) allowed the disappearance of the starting materials to be monitored as a function of reaction temperature, time, targeted molecular weight, catalyst …
-
The Development of a Novel Peptide-Drug Conjugate for Treating Triple-Negative Breast Cancer
… cleavable sulfo-SMCC linker. It was purified by reversed-phase HPLC and characterized by MALDI-TOF mass analysis. Its stability was studied in different conditions including aqueous-organic solvent mixture, human serum and mouse serum. The PDC was stable in aqueous-organic condition up to 10 …
-
Fourier transform infrared spectrometric detection of chromatographic effluents: instrumental and methodological improvements using a flow cell interface
… include High Performance Liquid Chromatography (HPLC), both normal-phase and reversed-phase, and packed-column Supercritical Fluid Chromatography (SFC). Normal phase HPLC-FTIR was demonstrated on not only analytical scale columns, but semi-preparative and microbore scales as well. Significant …
-
Evaluation of natural antioxidants
… Data in the literature was lacking so the phase partition coefficients (log P) of 53 phenolic antioxidants were measured by reversed-phase HPLC and calculated by log P prediction software. There was a very strong linear correlation between measured and calculated values (r=0.91). The …
-
Studies on the Formulation of Topical Corticosteroids
… the complex decomposition pathway, two normal phase and reversed phase HPLC systems were developed for assaying these two corticosteroids in the presence of their major decomposition products. The development and applications of these systems were discussed. The kinetics of decomposition were …
-
Proteomic Evaluation and Cytotoxicity of Red Maple (Acer rubrum) Leaves
… gel filtration fraction was conducted by reversed-phase HPLC. The crude and eluted fractions were analyzed by SDS-PAGE gel electrophoresis. The extract was screened for cytotoxicity activity on Michigan Cancer Foundation-7 breast cancer (MCF-7), M.D. Anderson Metastasis Breast (MDA-MB-231) …
Page 1 of 2