Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Reverse transcriptase Polymerase Chain Reaction"”.
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Application of Multiplex Reverse-Transcriptase Polymerase Chain Reaction for Identification of Leukemia Associated Gene Abnormalities
PUOPOSE: The best prognostic predictor for acute leukemia is known to be the finding of genetic abnormalities of leukemic cells. Methods for detecting the genetic abnormalities include chromosomal studies for karyotyping, FISH(Fluorescence in situ hybridization) and RT-PCR. However, each methods …
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Cell line development and multitrophic aquaculture of the lined seahorse, Hippocampus erectus
… frog virus 3, as demonstrated by quantitative reverse transcriptase polymerase chain reaction and tissue culture infectious dose titers. Multitrophic recirculation aquaculture systems (MRAS) were developed to reduce disease and labour and to improve economic benefit by simulating natural …
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Expression and functional role of cyclooxygenase enzymes in cervical carcinoma
… in cervical cancers. Real-time quantitative reverse-transcriptase polymerase chain reaction and Western blot analysis confirmed cyclooxygenase-1 and cyclooxygenase-2 ribonucleic acid and protein expression in all cases of squamous cell carcinoma and adenocarcinoma investigated. In contrast, …
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IDENTIFICATION OF HCMV UL97 IN GBM CELL LINES AND A POSSIBLE ROLE FOR GANCICLOVIR
… of HCMV phosphotransferase protein UL97. Reverse transcriptase polymerase chain reaction identified UL97 within two of the three cell lines, T98 and LN229. Western blotting confirmed that UL97 protein was being expressed and was present in both T98, and LN229 cell lines. UL97 is a …
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Studies of the mammalian ovarian endothelin system
… of bovine corpora lutea (CL). Semi-quantitative reverse transcriptase polymerase chain reaction was used to evaluate mRNA encoding ECE-1, ETA and ET-1. ECE-1 and ETA proteins were evaluated by semi-quantitative Western Blotting. ET-1 was the most likely target for PGF2 alpha regulation during the …
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Temporal Analysis of Bacteriophage Felix O1 Gene Expression
… of functional coding sequences via reverse transcriptase-polymerase chain reaction was performed. All of the 115 putative open reading frames (ORFs) studied were found to be functional. 53.0%, 9.6%, and 18.3% of the ORFs investigated were found to initiate expression early, middle or …
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The development of a flow cytometric method to detect the presence of mutated nucleophosmin in Acute Myeloid Leukaemia
… genetic abnormalities. Current methodology uses reverse transcriptase polymerase chain reaction (RT-PCR) and genomic deoxyribonucleic acid (DNA) PCR techniques to detect NPM1 DIM. Although these methods are robust and relatively easy to perform they can be expensive, labour intensive and not …
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Expression and Clinical Significance of the N-myc downstream regulated gene-1 in Hypopharyngeal Cancer
… of the hypopharynx with Ndrg-1 antibodies. For reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting, fresh normal and cancer tissue from the hypopharynx obtained from three patients were used. Results. Positive rates of Ndrg-1 expression in hypopharyngeal SCC were 21.4%, …
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The roles of hepatocyte growth factor family members in androgen-regulation of human hair growth. A comparison of the expression of hepatocyte growth factor family members, HGF and MSP, and their receptors, c-Met and RON, in isolated hair follicles from normal and androgenetic alopecia (balding) scalp.
… and analysed by molecular biological methods (reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative PCR and DNA microarray analysis), cell culture and immunohistochemistry. Scalp follicles expressed a range of paracrine messenger genes. The dermal papilla, cultured dermal …
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The effect of iron metabolism on palmitate-induced INS-1 cell death
… 7. RNA extraction and microarray analysis 27 8. Reverse transcriptase-polymerase chain reaction and quantitative PCR 28 9. DNA and RNA Transfection 28 10. Measurement of intracellular labile iron pool (LIP) 29 11. Aconitase activity assay 29 12. Oxygen consumption rate (OCR) 30 13. PA uptake 31 …
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Prognostic Value of Bone Marrow Micrometastasis Detected by Nnested RT-PCR for Cytokeratin 19 and Mammaglobin in Breast Cancer
… fraction from the samples and carried out nested reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of keratin-19 and mammaglobin mRNA with two different pairs of primer. After operation, the patients were followed up by 3-month intervals. We studied possible correlation …
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Stability And Recovery Of Rna In Biological Stains
… determined using quantitation of total RNA and reverse transcriptase polymerase chain reaction (RT-PCR) with selected housekeeping and tissue-specific genes. The results show that RNA can be recovered from biological stains in sufficient quantity and quality for mRNA analysis. The results also …
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Histone modifications and the Arabidopsis thaliana circadian clock
… (TOC1). Here, I used semi-quantitative Reverse Transcriptase Polymerase Chain Reaction (semi-quantitative RT -PCR) to investigate which enzymes are involved in the rhythmic expression of TOC1. I also determined whether loss-of-function histone acetylation and methylation mutants could …
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Detection of the bloodmeal origin of mosquitoes collected at alligator farms, and West Nile Virus activity in mosquitoes collected from alligator farms, sentinel chicken surveillance sites, and larval habitats
… total of 2, 404 mosquito pools were tested using reverse transcriptase polymerase chain reaction. West Nile virus was detected in 41 pools of females of 11 mosquito species. A comprehensive survey for vertical transmission of WNV was conducted. Male mosquitoes and mosquito larvae were collected in …
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TDI 천식 기도염증반응과 관련된 활성산소의 역할
… Immunoblot analysis 8 4. In vitro cross-linking reaction assay 9 5. Statistical analysis 9 B. Method 10 1. Materials 10 2. Cell culture 10 3. Real-Time polymerase chain reaction 10 4. Preparation of whole, cytosolic and nuclear extracts 11 5. Western blot analysis 12 6. Electrophoretic Mobility …
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A guinea pig model of human respiratory syncytial virus lung infection
… to test for RSV antigens and the reverse transcriptase-polymerase chain reaction (RT-PCR) to test for RSV genomic RNA. The experiments showed that juvenile guinea pigs inoculated with human RSV developed statistically significant bronchiolar inflammation by day 6 that resolved by …
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A study of the neurotoxicity of MPTP and analogs in human neuroblastoma SH-SY5Y cells
… and also caused an increased expression of the reverse transcriptase polymerase chain reaction (RT-PCR) product after treatment for 2 to 5 days at 10⁻³ to 10⁻⁴ M. The results, for the first time, demonstrated that MPTP affected cytoskeletal associated tau protein and altered its mRNA. These …
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Cytochrome P450 mRNA profile in human breast cancer cell lines.
… cells. This was achieved using a qualitative reverse transcriptase-polymerase chain reaction (RT-PCR) to detect mRNA expression of target genes. Results showed that CYP1B1, CYP2D6, CYP2J2, CYP2R1, CYP2U1 and CYP4X1 mRNA were present in all cell lines. The study also showed that CYP2A6, CYP2C8, …
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Gangliosides와 oxidized low density lipoprotein (oxLDL) 에 의한 신경교세포의 활성조절 및 그 기전에 대한 연구
… mobility shift assay (EMSA) 12 D. Reverse transcriptase-polymerase chain reaction (RT-PCR 13 E. Western blot analysis 15 F. Determination of NO release 15 G. Enzymatic digestion of sialic acid 16 H. Oxidation of LDL 16 I. Relative electrophoretic mobility (REM) assay 16 J. …
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The Effects of TET2-Deficiency on Neutrophil Gene Expression and Function
… (LTF), were assessed using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). MPO and LTF serum protein titers were quantified with enzyme-linked immunosorbent assays (ELISA). Additionally, the Tet2flox/flox Vav-iCre mouse model was used to investigate the effects of …
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