Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"Reverse phase HPLC"”.
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Electrochemical Detection of Aliphatic Sulfur Compounds in Liquid Coal Extracts
… thiols, and disulfides were studied using reverse phase HPLC with electrochemical detection in a acetonitrile/water mobile phase. Coal samples IBC 101 and IBC 105 were extracted with THF and hexane. The liquid coal extracts were fractionated using a simple chromatographic technique. The …
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The impact of degree of milling on the contents of rice bran lipids and gamma-tocotrienol
… AACC method and γ-tocotrienol was quantified by reverse phase HPLC. The results indicated that Cheniere had significantly higher contents of lipids and γ-tocotrienol than Francis at all degrees of milling (p≤0.05). It was also found that lipids and γ-tocotrienol content decreased with increasing …
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Factors affecting the accumulation of pentosidine in the skin of wild birds
… method and Ps was quantified using reverse phase HPLC. Ps concentration in the skin and foot webbing increased linearly with age (p < 0.001). Measurement of Ps in birds of unknown ages provided age estimates within the established life-span of these species. Hydroxyproline …
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A bioactive peptide QUB2177 from the defensive skin secretion of the frog, <i>Odorrana livida</i>
… was chemically-synthesised bymeans of solid phase peptide synthesis and structurally-characterised by application of MALDI-TOF MS. After reverse phase HPLC purification, the synthetic replicate of QUB-2177 was evaluated for its biological effect through functional assays. The results showed …
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Isolation, characterisation and molecular cloning of a bioactive peptide, QUB 1675, from the defensive skin secretion of Odorrana livida
… this peptide, QUB 1675 was synthesised by solid phase peptide synthesis (SPPS). The synthetic peptide mixture was purified by reverse phase HPLC and the molecular mass of this peptide was confirmed by MALDI-TOF MS. Antibacterial and antifungal properties of the purified synthetic peptide were …
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A novel bioactive peptide from the skin secretion of Hylarana nigrovittata
… QUB-1704 was synthesized by automated solid phase peptide synthesis and then subjected to MALDI-TOF analysis. Reverse-phase HPLC was used to purify the peptide. QUB-1704 showed no antimicrobial activity against three micro-organism models (E.coli, S.aureus, and C.albicans). Moreover QUB-1704 …
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Production of Copper-Complexing Ligands in Response to Elevated Concentrations of Copper in Marine <i>Synechococcus</i> spp.
… atomic absorption spectrophotometry, and reverse phase HPLC were used to isolate an intracellular copper binding component (CBC) of low molecular weight (Synechococcus PCC 73109 produced an intracellular CBC after exposure to [special characters omitted]8 μM CuSO<sub>4</sub> for 2 hr. The …
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QUB-2614: a bioactive peptide from the defensive skin secretion of Odorrana schmackeri
… chemically synthesised by the method of solid phase peptide synthesis (SPPS) and purified by reverse phase-HPLC.<br/><br/>Functional studies showed that QUB-2614 displayed antimicrobial activity against the Gram-negative bacterium, Escherichia (E.) coli, the Gram-positive bacterium, …
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Identification and bioactivity evaluation of a novel peptide from the skin secretion of Pelophylax kl. esculentus
… peptide was chemically-synthesised using solid phase chemistry and afterwards, its molecular mass and purity were confirmed by MALDI-TOF mass spectrometry and reverse phase HPLC. <br/>In pharmacological assays, QUB-1342 displayed a weak activity against S. aureus, with an MIC=64μM. It did not …
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Identification and functional evaluation of a bioactive peptide QUB-1369 Secreted from the skin of The Common Frog (Rana Temporaria)
… (FLPIVGKLLSGLL-NH2). Then, using Fmoc solid -phase peptide synthesis, the peptide chain was assembled stepwise and attached to resin support. After the peptide was assembled, it was removed from the resin to obtain a crude linear peptide. Reverse-phase HPLC was used to obtain a purified …
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Chemical modification and active site studies of Salmonella typhimurium phosphoribosylpyrophosphate synthetase
… CNBr and tryptic maps were prepared using reverse phase HPLC. Peptides from these maps were isolated and sequenced. About 85% of the primary amino acid sequence was covered. Previous work (Roberts et al., J. Biol. Chem, (1975) 250:5364-5369) suggested the presence of an essential cysteinyl …
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A bioactive peptide, QUB-2048, from the defensive skin secretion of Phyllomedusa tomopterna
… named QUB-2048 was isolated and identified by reverse-phase HPLC and MALDI-TOF mass spectrometry, and then chemically-synthesised by solid-phase peptide chemistry to facilitate assessment of bioactivity.<br/>QUB-2048 was found to have potent antimicrobial activity against the yeast, Candida …
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A peptide, QUB-1420, from the defensive skin secretion of Heilongjiang Brown Frog Rana amurensis
… of cDNA ends (RACE) method. After that, solid phase Fmoc-based chemistry method was used to synthesise the peptide for the following functional experiments. The reverse-phase HPLC was employed to purify the synthetic peptide products. After a range of bioassays assessment, the antimicrobial, …
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Assessing bioactivity of a bioactive peptide from the defensive skin secretion of Odorrana schmackeri
… and isolated from the skin secretion sample by reverse-phase HPLC fractionation. The molecular weight of the peptide was then confirmed by using MALDI-TOF mass spectrometry. Solid phase peptide synthesis (SPPS) was employed to chemically synthesise the peptide. Finally, antimicrobial, anticancer …
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Mechanistic investigation of polyhydroxybutyrate (PHB) synthases and elucidation of PHB biosynthesis and degradation process in Wautersia eutropha H16
… The initiation, elongation, and termination phases of this non-template driven polymerization process are not well understood. This thesis is focused on the initiation and the elongation phases leading to granule formation. For the first time, we have observed intermediate species in the in …
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On-line liquid distribution chromatographic/spectrofluorometric determination of PAH distribution in coal-derived products
On-line HPLC/Spectrofluorometric techniques have been applied to the determination of polycyclic aromatic hydrocarbons (PAHs) in coal-derived products. The PAHs were isolated by preparative silica gel columns with a hexane mobile phase, and fractionated into five samples based on the number of …
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Identification and functional study of a novel antimicrobial peptide, QUB-2014, from the skin secretion of the frog, Phyllomedusa tomopterna
… that, the peptide was synthesised through solid-phase peptide synthesis, and the crude peptide was purified via reverse-phase HPLC (RP-HPLC). MALDI-TOF mass spectrometry was used further to confirm the target peptide from the RP-HPLC fractions. Significantly, antimicrobial assays showed that this …
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Effect of Fatty Acid Modification on Prostacyclin Production in Cultured Human Endothelial Cells (Arachidonate, Elcosapentaenoate)
… fatty acid eicosanoid products were separated by reverse phase HPLC. Unconverted fatty acid was the major product released upon thrombin stimulation in both cases. ('3)H-AA supplemented cells also released PGI(,2) and HETE in lesser amounts while ('14)C-EPA supplemented cells released only minimal …
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Proteomics and Biological Evaluation of Marshmallow (Althea officinalis) Seeds
… by the combination of gel filtration and reverse-phase HPLC. Purified proteins were analyzed by SDS-PAGE gel electrophoresis. The partial amino acid sequence of 22 kDa protein was established up to 25 residues by N-terminal amino acid sequencing. The data suggest that Marshmallow RP-HPLC …
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Studies on bovine γ-glutamylamine cyclotransferase.
… to the enzyme was carried out by microcapillary reverse-phase HPLC nano-eletrospray tandem mass spectrometry; 42 proteins and protein fragments of similar mass and pI as that of γGACT were obtained. Analysis of their properties indicates that the unknown protein for MGC:134378 is the most likely …
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