Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Reverse genetics system"”.
-
The development of a reverse genetics system for Machupo virus
… and large segments, and the establishment of a reverse genetics system and rescue of a recombinant MACV. Using these tools, I present the first modern in vitro characterization of MACV, the development of a novel and lethal murine animal model, and the generation of a rationally attenuated MACV.
-
Reverse genetics system and fucntion of NSM protein of Rift Valley fever virus (family Bunyaviridae, genus phlebovirus)
… I established a T7 RNA polymerase-based reverse genetics system to rescue infectious clones of RVFV MP-12 strain entirely from cDNA, the first for any phleboviruses. Using this system, RVFV carrying substitution mutations of the M gene preglycoprotein region (pre-Gn region), one lacking …
-
Identification of Viral Determinants of Murine Norovirus Pathogenesis
… model, can be grown in cell culture, and has a reverse genetics system. Noroviruses are divided into genogroups which can contain many viral strains. These viral strains can display dramatic variation in their virulence and ability to persist, but the factors responsible for these differences …
-
DEVELOPMENT OF AVIAN PARAMYXOVIRUS VECTORED VACCINES
… In the first part of my study, I created a reverse genetics system for Avian paramyxovirus-3 strain Wisconsin (APMV-3 Wisc.) which was proven to be nonpathogenic for day-old chicks and embryonated chicken eggs. The virus was used as a vector to express the enhanced green fluorescent protein …
-
Exploring Nipah virus strain differences through use of recombinant and chimeric viruses as well as utilization of a novel 3D human lung model
… address this gap. In addition to constructing a reverse genetics system for NiVB (rNiVB) to complement the previously available system for NiVM, a panel of chimeric NiVs expressing the fusion (F), attachment glycoprotein (G), and matrix (M) proteins of the alternate variant alone or in …
-
Spike Processing and Protease Usage Effects on SARS-CoV-2 Pathogenesis
… contribute to SARS-CoV-2 pathogenesis. Using our reverse genetics system, we generated several infectious clones to examine the various features of the CTS1: ΔQTQTN demonstrated the importance of loop length and glycosylation. PQQA established that the integrity of the FCS is necessary. Omicron …
-
Evaluation of the potential functions of Avian paramyxovirus Accessory proteins
… lengths, rescued recombinant viruses by reverse genetics system and tested for thermostability. Our results showed that W protein does not confer thermostability to NDV. In the second part of study, we constructed plasmids expressing APMV -2, -3 and -6V proteins and tested for IFN …
-
Genetic Determinants of Junin Virus Attenuation
… To this end, an RNA polymerase I/II-based reverse genetics system was utilized to rescue the wild type pathogenic Romero and attenuated Candid #1 strains of JUNV. Both recombinant viruses exhibited similar in vitro growth kinetics and in vivo biological properties to their parental …
-
Host and viral determinants of flavivirus emergence and pathogenesis
… transmission capacity in Aedes aegypti. Using a reverse genetics system to engineer the amino acid changes of these viruses into a ZIKV infectious clone, I define the contributions of these genetic changes to the enhanced virulence and transmission phenotype using in-vivo mouse and mosquito …
-
Molecular biology of segment 8 of the influenza A virus genome
… it may exert on viral replication. Using a reverse genetics system for the A/Puerto Rico/8/34 influenza A strain, mutant viruses were generated containing negative strand open reading frames of different lengths, as well as one with early stop codons that result in a severe truncation. All …