Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 203 for “"Reverse Transcription"”.
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Studies of Entry, Reverse Transcription, and Regulation of Splicing in Retroviruses
… made. The same enzyme that performs this step, reverse transcriptase (RT), also revolutionized molecular biology when it was used as a tool to generate full length cDNA clones of expressed genes. The impact of retroviruses on the medical field has been extremely exciting as the ideas of using …
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An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.
… countries. This study was aimed at evaluating Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) as a viable alternative method to RT-PCR and DFAT for diagnosis of rabies in Zambia. RT-LAMP primers were designed based on the most conserved region of N gene sequences covering …
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An evaluation of reverse transcription loop-mediated isothermal amplification (RTLAMP) for diagnosis of rabies.
… countries. This study was aimed at evaluating Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) as a viable alternative method to RT-PCR and DFAT for diagnosis of rabies in Zambia. RT-LAMP primers were designed based on the most conserved region of N gene sequences covering …
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Biochemical Investigation of the Intracellular Trafficking of Non-Viral and Hybrid Gene Therapy Vectors
We also used reverse transcriptase inhibitor 3'-azido-3'-deoxythymidine (AZT) applied of post-transfection time increments to determine the timeframe of reverse transcription, thereby estimating endosomal escape of VLP. MLV were observed to achieve reverse transcription rapidly, with more than half …
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Studies into the role of capsid serine/threonine residues in HIV core stability and virus replication.
… some CA remains associated with the resulting reverse transcription (RTC) and preintegration complexes (PIC). What triggers release of CA from the core is unknown. Cores from virus containing mutations in CA that show altered core stability, and release CA from the core at rates faster or …
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Defect of Mitochondrial Translation Machinery Enhances Hepatoma Cell Invasiveness
… drug treatment and cell growth rate 4 2. Reverse transcription-polymerase chain reaction (RT-PCR) 4 3. Endogenous cellular oxygen consumption rate 5 4. Estimation of intracellular and mitochondrial ROS level 5 5. Total genomic DNA isolation and sequencing of mitochondrial DNA fragments 5 …
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Cell tropism of maedi-visna virus
… and contains enhancer sequences that cellular transcription factors can bind to. It is therefore likely that this difference between the two virus strains is because of transcription regulation. The loss of replication activity in SCP cells that we see in the viruses without a repeat in the LTR …
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Interactions Between APOBEC3 and Murine Retroviruses: Mechanisms of Restriction and Drug Resistance
… to hypermutation of viral DNA synthesized during reverse transcription. These mutations can result in viral lethality via disruption of critical genes, but in some cases is insufficient to completely obstruct viral replication. This sublethal level of mutagenesis could aid in viral evolution. A …
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Characterization and Regulation of Insulin-Like Growth Factor-Imrna Transcripts in Myeloid Cells
Using reverse transcription and PCR amplification, cDNA fragments corresponding to IGF-I Ea and Eb mRNA transcripts were detected in all murine immune cell types. The identity of these sequences was confirmed by cloning and sequencing. Macrophages had 20 to 40 fold higher levels of IGF-I mRNA than …
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NMR studies of RNA binding domains of human lysyl aminoacyl tRNA synthetase
… is to target lysyl tRNA isoacceptor 3, the HIV-1 reverse transcription primer molecule, for uptake and packaging into new HIV-1 particles. Since the anticodon binding (ACB) domain of hLysRS is required for proper recognition of its cognate tRNA, NMR studies of the ACB domain were conducted to …
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INTERACTIONS BETWEEN THE GLYCOSYLATED GAG PROTEIN OF A MURINE LEUKEMIA VIRUS AND MURINE APOBEC3: NOVEL INSIGHTS INTO HOW A MURINE LEUKEMIA VIRUS COUNTERACTS A RESTRICTION FACTOR
… been identified, such as directly inhibiting reverse transcription. Both HIV and murine leukemia viruses (MuLVs) have evolved mechanisms to evade the action of the APOBEC proteins. HIV encodes the Vif protein which binds to hA3G and facilitates its rapid degradation through the proteasome. The …
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INTERACTIONS BETWEEN THE GLYCOSYLATED GAG PROTEIN OF A MURINE LEUKEMIA VIRUS AND MURINE APOBEC3: NOVEL INSIGHTS INTO HOW A MURINE LEUKEMIA VIRUS COUNTERACTS A RESTRICTION FACTOR
… been identified, such as directly inhibiting reverse transcription. Both HIV and murine leukemia viruses (MuLVs) have evolved mechanisms to evade the action of the APOBEC proteins. HIV encodes the Vif protein which binds to hA3G and facilitates its rapid degradation through the proteasome. The …
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Epigenetic repression of intronless mobile elements by the HUSH complex
… cells recognize incoming DNA and limit its transcription through repressive chromatin modifications. The human silencing hub (HUSH) complex transcriptionally represses long interspersed element-1 retrotransposons (L1s) and retroviruses through histone H3 Lys9 trimethylation (H3K9me3). How …
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DNA target site recognition by the Ll.LtrB group II intron RNP
… at a specific DNA target sequence and is then reverse transcribed by the IEP. Both the intron RNA and IEP are required for DNA target site recognition. I have identified the contact sites within the IEP responsible for recognition of two key positions in the DNA target, T+5 and T-23. IEP …
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Host Proteins Interact with the Hiv-1 Core to Facilitate and Restrict
… (HIV-1) soon after viral entry but prior to reverse transcription (RT). It is well established that the restriction requires an interaction between the viral capsid lattice and the B30.2/SPRY domain of TRIM5á. Following the binding of the viral core, TRIM5á mediates an event or series of …
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Macrophage differentiation: A heterogeneous theory
… from individual clones was monitored using the reverse transcription polymerase chain reaction (RT-PCR) to permit simultaneous amplification of multiple specific gene transcripts. Internal ""nested"" primers were utilized in addition to the two traditional external primers thus, increasing the …
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Specific diagnosis for effective treatment of neuroblastomas and pulmonary neuroendocrine tumors through transcriptional profiling-based interpretation of cellular development
… factors will alter their growth potential. Using reverse-transcription polymerase chain reaction (RT-PCR) analysis, the present study compared the expression of about 50 regulatory genes that are expressed at early stages of neurogenesis in five human NBs and NETs, also upon GDNF/TGF-β1 and …
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