Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"Retrotransposition"”.
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Somatic retrotransposition in the cancer genome
… myeloid leukemia show no evidence of somatic retrotransposition. Moreover, many somatic retrotransposon insertions occur in known cancer genes. TranspoSeq-Exome uncovers 35 additional somatic retrotransposon insertions into exonic regions, including an insertion into an exon of the PTEN tumor …
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Alu retrotransposition-mediated genomic variation within the primate order
… genomic instability was discovered, termed retrotransposition-mediated deletion. A computational search within the draft human and chimpanzee genomes found evidence of 33 retrotransposition-mediated deletion events that have eliminated approximately 9,000 nucleotides of genomic DNA. During …
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Restriction of Retrotransposition and Retroviral Infection by RNase L and APOBEC3G
… a nuclease-dead RNase L mutant (R667A), impaired retrotransposition of both human LINE-1 and mouse IAP elements (Chapter 2). Depletion of endogenous RNase L with siRNA increased levels of LINE-1 retrotransposition. Wild type RNase L, but not RNase L R667A, dramatically reduced L1 RNA accumulation, …
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THE ROLE OF RETROTRANSPOSITION AND EXOSOMAL DNA IN RADIATION-INDUCED GENOMIC INSTABILITY
… doses of 0.1 Gy and 2 Gy X-rays activated LINE-1 retrotransposition, and if this caused an instability within the genome. 48 hours post exposure to 0.1 Gy X-rays the HF19 fibroblast cell line showed an increase in LINE-1 levels, which correlated with an increase in micronuclei formation. While …
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Fanconi Anaemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mammalian development
… humans and represents 17% of the genome. LINE-1 retrotransposition occurs by a “copy and paste” mechanism in which an RNA intermediate is reverse-transcribed and integrates at a new genomic location. LINE-1 elements have shaped the evolution of the human genome but are often deleterious causing …
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The relative retrotransposition efficiencies of human and mouse short interspersed DNA elements (SINEs) in a HeLa cell culture assay
… which has been supported by a cell culture-based retrotransposition assay. SINEs are ancestrally derived from either the 7SL RNA gene or from various tRNA genes. Sequence data support the co-evolution of some tRNA-derived SINEs with LINEs. This study involved testing whether or not tRNAderived …
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A proteomic approach for studying LTR retrotransposon host factors in saccharomyces cerevisae
… genetic structures and replication cycles. Ty retrotransposition begins in the nucleus with the transcription of Ty RNA. Following translation of Ty proteins, virus-like particles (VLPs) assemble in the cytoplasm. VLPs contain Ty RNA and enzymes surrounded by a capsid shell. Specific cellular …
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DNA as a Catalyst and as a Sensor: Investigating the Structure of the Ty1 Retrotransposition Intermediate and the Utility of DNA -Based Biosensors
In vitro selection has been utilized to develop aptamers and aptazymes as components of small-molecule sensors. Aptamers have been selected for a range of targets from proteins to small-molecule ligands. Aptazymes have an aptamer domain that can allosterically control the catalysis of an attached …
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DNA as a Catalyst and as a Sensor: Investigating the Structure of the Ty1 Retrotransposition Intermediate and the Utility of Dna-Based Biosensors
… putative 2',5'-branched RNA intermediate in Ty1 retrotransposition. Aptamers are three-dimensional structures of single-stranded DNA that can bind small molecules with both high affinity and selectivity. Aptamers are isolated by in vitro selection from random libraries of single-stranded …
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Transcriptional and Post-Transcriptional Regulation of Retroelements
… addition to actual genomic integration events, retrotransposition intermediates like RNA, dsRNA, RNA:DNA hybrids and reverse transcribed cDNA are also potentially oncogenic due to induction of inflammatory response pathways and there exists multiple post-transcriptional regulatory pathways that …
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RNA helicase MOV10 is essential for gastrulation and normal CNS development
… studies that propose an increase in endogenous retrotransposition in fetal brain as well as in the hippocampus and other regions of the adult brain during neuronal differentiation. Furthermore, L1 retrotransposition is more common in brain compared to other tissues in the body. Despite the many …
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Regulation of exogenous retroviruses and endogenous retroelements by MOV10
… that natural levels of MOV10 suppress retrotransposition. MOV10 overexpression decreases the level of HIV-1 genomic RNA packaged into nascent virions and also impacts the accumulation of reverse transcription products in target cells. The molecular mechanism/s by which MOV10 inhibits …
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Post-transcriptional coordination by an RNA-binding protein
… cell cycle, and cell wall functions. Tyl element retrotransposition is also induced under conditions of nitrogen starvation. I describe a role for the RNA-binding protein Khdl in regulating these two responses to environmental stress through its mRNA targets. I identified the RNA targets of Khdl …
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Regulation of the ETn/MusD family of active mouse long terminal repeat retrotransposons
… retroviral genes but are transcriptionally and retrotranspositionally active, causing over 20 known insertional germline mutations. ETns owe their retrotransposition potential to proteins encoded by structurally intact MusD retrotransposons with whom they share LTRs. ETn elements are transcribed …
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Mammalian-wide interspersed repeats (MIRs) and their role in mammalian gene function and evolution
… accumulate in the genome via a process termed retrotransposition, wherein the elements are reverse transcribed into RNA and inserted into the genome as DNA. Exaptation of these elements can provide additional or novel function for endogenous genes. Mammalian-wide interspersed repeats (MIRs) are …
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Transposable Elements in Neural Progenitor Cells
… studies report Line-1 transcription and frequent retrotransposition during normal brain development, with further evidence that Line-1 induced retrotransposition can influence neuronal gene expression. Today, there is few published data focusing on epigenetic regulation of transposable elements in …
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Zebrafish cardiovascular cDNA microarrays : expression profiling and gene discovery in embryos exposed to 2,3,7,8-Tetrachlorodibenzo-ρ-dioxin
… elements lack genes necessary for autonomous retrotransposition, but are highly expressed in normal and TCDD-exposed cardiac tissue. Putative regulatory elements in LTR sequences may account for observed expression patterns. The function, if any, of EZR1 remains open to speculation.
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Genetische und funktionale Vereinfachung eines komplexen Retrovirus am Beispiel des Primaten Foamy Virus Typ 1 (PFV-1)
… wie der Möglichkeit zur intrazellulären Retrotransposition oder der reversen Transkription spät im Vermehrungszyklus, nehmen sie eine funktionale Sonderstellung zwischen Retro-, Hepadnaviren und Retrotransposons ein. Aufgrund des Aufbaus ihres Genoms, das neben dem minimalen Gensatz der …
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Following the LINEs (Long INterspersed Elements): human specific L1 elements and their orthologous loci in non-human primates
… L1 element, as well as several potential retrotransposition competent elements. The average age of the L1 Ta subfamily was estimated at 1.99 million years, indicating the subfamily’s expansion subsequent to the divergence of humans from African apes. PCR based screening in non-human …
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Farnesylation-dependent regulation of transcripts by PARP13
… the miRNA silencing pathway, restricts human retrotransposition by regulating LINE-1 mRNA and downregulates the mRNA levels of TRAIL-R4 -a pro-survival TRAIL ligand receptor. Farnesylation is a post-translational lipid modification that causes proteins to associate to membranes and is required …
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