Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"Reticulocyte"”.
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Cell free synthesis of ferritin using the Modified Reticulocyte Lysate System.
Thesis: M.S., Massachusetts Institute of Technology, Department of Nutrition and Food Science, 1978
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From the marrow to the blood: Optimising the diagnosis of iron deficiency in the setting of inflammation
… Laboratory markers including red cell indices, reticulocyte haemoglobin and iron studies were evaluated to establish optimal cut-offs for ID diagnosis. A CRP of >5 mg/L was used as a marker of inflammation. The study included 139 patients. Forty-two patients had BM ID with a median serum …
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A Pseudoknot Improves Selection Efficiency in Ribosome Display
… protein. During in vitro translation in rabbit reticulocyte lysate, I observed that incorporation of the pseudoknot into the mRNA resulted in production of a translational intermediate that corresponded to the expected size for ribosomal arrest at the pseudoknot. Complexes containing the mRNA …
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Application of in vitro erythropoiesis from bone marrow derived progenitors to detect and study genotoxicity
… (BM) using the metric of genotoxic damage to the reticulocyte population in mice. An in vitro correlate to this assay might facilitate extension to human cells and thus provide a highly predictive genotoxicity assay. As first steps in developing a toxicity assay, this thesis work (a) adapted a …
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Mechanism of transmissible gastroenteritis virus nsp1-mediated translation inhibition
… mRNAs, in HeLa S10 extract but not in rabbit reticulocyte lysate or wheat germ extract. In this dissertation the mechanism of TGEV nsp1-mediated translation inhibition is further elucidated. Immunofluorescence analysis showed that TGEV nsp1 protein localizes to both the nucleus and cytoplasm …
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Mechanism of Translational Control by the Fragile X Mental Retardation Protein and Creation of the FMRP CTAG Mouse
… stalling ribosomes, both in vivo and in rabbit reticulocyte lysate programmed with endogenous brain polyribosomes. In these systems, loss of FMRP function resulted in increased ribosome runoff after treatment with puromycin, a drug that acts specifically on translocating ribosomes. In addition …
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Identification and molecular characterisation of a putative gene for cell detaching factor from Trichomonas vaginalis.
… using T7 RNA polymerase and translated in rabbit reticulocyte lysate in the presence of 35S-methionine. Several high molecular weight proteins (>200, 165, 150, 130, 118, 80, 47 kDa) were produced. Two polypeptides of 205 and 220 kDa were immunoprecipitated from the in vitro translation mix by the …
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Expression Of Growth Factor Inducible Genes
… generated by SP6 polymerase, and translated in reticulocyte lysates. The in vitro translated TIMP was immunoprecipitable with TIMP antiserum. TIMP was translated up to 10-fold more efficiently from the transcript lacking the upstream AUG.;RNA was isolated from the reproductive tissues dissected …
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Alterations in cerebellar protein synthesis in propylthiouracil-induced hypothyroid rats
… A RNA isolated and translated in an in vitro reticulocyte lysate system. The specific proteins were immunoprecipitated and analyzed by SDS-PAGE. To correlate these results with the amounts of mRNA's for apo E and T1, northern hybridization was performed. Both apo E and T1 levels synthesized in …
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The Role of Queuine Deficient tRNA in Translational Control
… populations of tRNA to supplement rabbit reticulocyte lysate translation assays. The mRNA was produced in a transcription assay using the bacteriophage T3 polymerase to transcribe the cDNA of genes associated with growth and transformation. This allowed a comparison of the amount of …
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Engineering control of eukaryotic translation with application to the malaria parasite Plasmodium falciparum
… Saccharomvces cerevisiae and cell-free rabbit reticulocyte extracts. We show the broad utility of the system in P. falciparum for controlling expression of reporter and endogenous proteins trafficked to a variety of subcellular compartments. Induction and repression are rapid and homogeneous …
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Receptor- G Protein Interactions in the Visual System: A Study of Structures and Mechanisms That Couple the Cytoplasmic Surface of Rhodopsin to the Nucleotide-Binding Pocket of Transducin
… The mutants were expressed in vitro in rabbit reticulocyte lysate and the kinetics of both basal and R*-catalyzed nucleotide exchange were determined by quantitative analysis of trypsin digest patterns. Mutations in a series of residues at the interface between the two domains of Gαt had only …
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Characterisation of two aphid picorna-like viruses
… RhPV RNAs were efficiently translated in rabbit reticulocyte lysate into high molecular weight polypeptides, the sum of which exceeded the coding capacity of the genomes. Putative capsid precursor proteins of ALPV and RhPV were identified by immunoprecipitation. ALPV translation products were …
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Exosporium Morphogenesis in Bacillus Cereus and Bacillus Anthracis
… mass spectrometry. A novel cell surface protein, reticulocyte binding protein (Rbp), was identified in this sample. Inactivation of rbp by insertional mutagenesis resulted in spores devoid of the hair-like nap typical of the exosporium, which suggests that Rbp could be a new component of the …
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Direct Recruitment of eIF4GI and/DAP5 to the 5' UTR of a Subset of Human mRNA Drives Their Cap-independent Translation
… an eIF4GI or DAP5-dependent manner in a rabbit reticulocyte lysate depleted of eIF4GI or DAP5. We also show how translation of this subset of mRNAs with structured 5’ UTRs are either dependent on the availability of an exposed 5’ end or not, allowing us to distinguish between IRES- or …
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The anaemia of pregnancy: A report on the haematological study of 48 cases of pregnancy, with review of the literature
… Red cell counts, haemoglobin estimations, reticulocyte counts, white cell counts, and estimation of plasma bilirubin were part of the routine investigation.
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A microfluidic biosensor to electrically enumerate blood cells at point-of-care for infectious disease diagnosis and management
… the same setup can be used in the future for reticulocyte counting that measures the health of bone marrow, especially for individuals undergoing chemotherapy. For the next step in translating our differential immuno-capture technology, we have explored the sepsis diagnosis application. …
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New approaches to understanding the interactions between Plasmodium falciparum merozoites and human erythrocytes during invasion
… of the EBA-175 like Antigen (PfEBA) family and Reticulocyte Binding Protein Homologue (PfRH) invasion ligand families did have a significant negative impact on attachment. Deletion of individual PfEBA and PfRH ligands reinforced the known redundancy within these families, with the deletion of …
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Non-lysosomal protein degrading systems in chicken skeletal muscle
… lower than those detected in rabbit reticulocytes. Conjugation of ubiquitin to a subset of endogenous proteins was detected by incubating muscle extracts (fraction II: depleted of ubiquitin by DEAE-cellulose chromatography) with 125I-ubiquitin and Mg2+-ATP, followed by analysis of the …
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