Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Restriction-modification systems"”.
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Non-parental-type Recombinants in Crosses between Different Restriction-Modification Systems in SALMONELLA
<p>Many new restriction-modification (R-M) system which are closely linked to <em>serB</em> and allelic to the <em>hsd</em><sub>SB</sub> system of <em>S. typimurium</em> occur in different <em>Salmonella</em> serotypes. In PI transduction studies with these systems, recombinants were isolated which …
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Deoxyribonucleic acid restriction/modification systems and gene transfer strategies in Ruminococcus albus 8 and Ruminococcus flavefaciens FD-1
… FD-1. The limited degradation of DNA by restriction enzymes and confirmation of plasmid uptake were given priority, although the consequences of incompatible plasmid replicons and failure in the expression of selectable markers cannot be overlooked. Fluorescent labelled dextrans were used …
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Investigating Epigenetics as a Mechanism to Regulate Bacterial Heterologous Expression
… transcription. Epigenetic methylation as part of restriction-modification systems is frequently considered a barrier in synthetic biology since it can prevent the introduction of heterologous genes to new host organisms. Due to this, many studies that construct bacterial hosts for use in synthetic …
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Genetic studies of Streptomyces cattleya and Streptomyces olivaceus
… lividans TC10 indicated the absence of restriction-modification systems which affect VCll infectivity of these Streptomyces strains.
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A Fast Assay of Bacteria Cell Permeability for Genetic Transformation
… from confounding factors such as restriction-modification systems. As a result, it provides a more accurate qualitative and quantitative assessment of bacterial membrane disruption. This approach enables same-day evaluation of electroporation conditions regardless of bacterial …
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Prevotella Phylogeny: Genomic And Molecular Insights Into The Role Of The Human Commensal Prevotella In Cystic Fibrosis
… have high recombination rates but also complex restriction-modification systems. Our work highlights the incredible genomic diversity within some of the oropharyngeal commensal Prevotella, indicating that the key to drawing meaningful associations of Prevotella with health and disease is by …
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The evolutionary ecology of methylation-dependent bacterial defense systems
… has driven the evolution of diverse host defense systems. Although recent years have seen a rapid expansion in defense system discovery, there remain considerable gaps in our understanding of the evolutionary ecology of MGEs and host defenses. Given the role of MGEs in spreading drug resistance …
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Comparative genome analysis of human bifidobacteria with commercial potential
… novel B. longum subspecies. Characterisation of restriction-modification systems from two B. longum subsp. longum strains is described in Chapter 3. These defence mechanisms limit the uptake of genetic material, which was successfully demonstrated for some of the identified systems. When these …
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The development of Sinorhizobium meliloti and Deinococcus radiodurans as chassis for synthetic biology applications
… its utility through the generation of robust systems for conjugation-based genome engineering and whole genome cloning in vivo. Using this method, I cloned the large (178 kb) MP1 megaplasmid from D. radiodurans in E. coli. Finally, I developed a strategy to create seamless gene deletions in D. …
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Anti-CRISPRs and CRISPR-Cas: Characterization and Biotechnology
… These include inhibiting phage adsorption, restriction modification systems, and the more recently discovered CRISPR-Cas immune systems. Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci together with their accompanying CRISPR-associated (Cas) genes form the only known …
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Mechanistic studies of Fusobacterium genetic and defense systems
… exploits innate and adaptive defense systems present in Fusobacterium for their use as molecular tools for genome editing. Accordingly, we first investigated restriction-modification systems with a focus on the role of DNA methyltransferases and endonucleases in host defense and …
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On the Repair of DNA Breaks and the Specificity of the EcoRI Restriction Enzyme
… our studies of the Mrr methylation dependent restriction system, DNA single- and double-strand break repair in E. coli, and substrate recognition by the EcoRI endonuclease. Many species of bacteria make restriction-modification systems to destroy foreign DNA that enters the cell. These systems …