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Showing 1 to 20 of 34 for “"Restriction fragment length polymorphism (RFLP)"”.

  1. BoLA Class II Genes: Polymorphism, Disease Association and Linkage Relationships With the Prolactin and Bola-A Genes

    Van Eijk, Michael Josephus TheresiaUniversity of Illinois at Urbana-Champaign2014

    … II genes of the BoLA complex than with BoLA-A. Polymorphisms of BoLA-DQB and -DRB2 genes were identified using restriction fragment length polymorphism (RFLP) analysis. All carriers of DRB2*2A were found to be resistant to PL (P $<$ 0.005), whereas DRB2*1C homozygotes were at higher risk for the …

    uiuc Repository record

  2. Assessment of Chemiluminescent Detection System for Forensic DNA Restriction Fragment Length Polymorphism Analysis

    Bush, Paul J.Drake University1984

    … of 32P isotopic detection for human DNA restriction fragment length polymorphism (RFLP) analysis and have the speed, safety, and reduced cost of colorimetric detection. The assessment was made on the basis of: cost, sensitivity, time of operation, ease of operation, and ease of multiple …

    drake Repository record

  3. Associations of egg production with the major histocompatibility complex in broiler breeder hens

    Tarleton, Becky Jean2019

    … hens. Genomic DNA was isolated from 35 hens. Restriction fragment length polymorphism (RFLP) analysis was performed on each sample, using a chicken class II MHC probe (Xu et al. 1989). Restriction endonuclease digestion using PvuII resulted in polymorphisms in all birds. Five polymorphic bands …

    wvu Repository record

  4. New insights on the ecology of free-living heterotrophic nanoflagellates based on the use of molecular biological approaches

    Lim, Ee LinMassachusetts Institute of Technology and Woods Hole Oceanographic Institution1997

    … of nanoflagellate taxa in water samples. Restriction fragment length polymorphism (RFLP) analysis of small subunit ribosomal DNA (SSU rONA) amplified by the polymerase chain reaction (PCR) differentiated pure cultures of heterotrophic nanoflagellates according to established taxonomic …

    woods-hole Repository record

  5. Combining ability, protein, heterosis, and prediction of F₁ performance with RFLPs in a diallel of maize

    Ball, Dale WarrenVirginia Tech1994

    … by additive gene action. In the second study, restriction fragment length polymorphism (RFLP) data were obtained for the 12 inbreds using 42 genomic clones each with four restriction enzymes. Modified Roger's distances were calculated and used in cluster analyses for heterotic grouping of the …

    vt Repository record

  6. An evaluation of DNA fingerprinting methods for subtyping Salmonella

    Burr, Mark Daniel, 1949-The University of Arizona.1996

    … DNA-based methods, including plasmid analysis, restriction fragment length polymorphism (RFLP) analysis, and polymerase chain reaction (PCR) fingerprinting methods were evaluated. Plasmid analysis, including plasmid profiles and plasmid fingerprints have been shown to be useful primarily in …

    arizona-thes Repository record

  7. Molecular, Genetic, and Agronomic Evaluation of Wp and Wp-M in Soybean

    Hegstad, Jeffrey MarshallUniversity of Illinois at Urbana-Champaign2015

    … The cloned 3'5 ' flavonoid hydroxylase enzyme fragments from soybean did not show restriction fragment length polymorphism (RFLP) between flower color or pubescence isolines. A molecular marker linkage group for wp was constructed using two RFLP and thirteen simple sequence repeat (SSR) …

    uiuc Repository record

  8. Identification of fungi and bacteria associated with internally discolored horseradish roots

    Yu, JunmyoungUniversity of Illinois at Urbana-Champaign2010

    … resulting from polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) assay. A total of 52 bacterial species in 20 genera were identified, which were classified into five classes, Actinobacteria (50 roots), Alphaproteobacteria (10 roots), Bacilli (234 roots), …

    uiuc Repository record

  9. High-Resolution Mapping of the Region around the Soybean Virus Resistance Genes, Rsv1 and Rpv1

    Gore, Michael AllenVirginia Tech2000

    … polymorphic DNA (RAPD), five SSR, and 21 restriction fragment length polymorphism (RFLP) markers. Included in these RFLP markers were seven resistance gene candidate (RGC) and five resistance gene candidate flanking (RGCF) markers. RGC probes encode a protein with homology to previously …

    vt Repository record

  10. Functional study of ubiquitin C-terminal hydrolase-L1 gene promoter haplotypes

    Sanassy, ShaneUniversity of Southampton2007

    … novel UCH-L1 Single Nucleotide Polymorphisms (SNPs), as well as the C54A documented coding region polymorphism (Ser18Tyr), were identified using both denaturing High Performance Liquid Chromatography (dHPLC) and DNA sequencing analysis. In relation to the translational start …

    soton Repository record

  11. An investigation of genetic polymorphism in association with Type 2 diabetes and metabolic syndrome.

    Bhatta, PrabhakarRobert Gordon University2018

    … the putative association of single nucleotide polymorphisms with Type 2 diabetes (T2D), metabolic syndrome (MetS) and the major components of metabolic syndrome. This study used metaanalysis, polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP) and Sanger …

    rgu Repository record

  12. Polymorphic Variation of the DGAT1 Gene in Cal Poly Holstein and Jersey Breeds and the Correlation of Milk Fat Content, and Genetic Values

    Laubscher, Andrea M.2011

    … in the centromeric end of chromosome 14. The polymorphism being analyzed is a K232A substitution (lysine to alanine amino acid change) in exon VII of the diacylglycerol acyl transferase 1 (DGAT1) gene. DGAT1 is a key acyltranferase in triglyceride biosynthesis. DGAT1 encodes an enzyme which …

    calpoly Repository record

  13. Molecular Mapping Of A Soybean Mosaic Virus (SMV) Resistance Gene In Soybean (Glycine Max)

    Kristipati, Sesha Sai VenkataVirginia Tech2004

    … lines (NILs), and bulk segregants with 136 restriction fragment length polymorphism (RFLP) markers yielded 36 markers showing variation between the two parents. These polymorphic RFLP markers unable to provided any indication of linkage to Rsv3. As an alternative strategy, amplified fragment …

    vt Repository record

  14. Avaliação da etiologia das infecções enteroparasitárias em diferentes grupos pediátricos e genotipagem de isolados de Giardia duodenalis

    Silva, Renata Kelly Novais RodriguesFaculdade de Farmácia2017

    … β-giardina and Gdh, followed by Analysis of Restriction Fragment Length Polymorphism (RFLP). Among the 824 children, 30.3% were infected with at least one parasite. Children with cancer had the highest frequency of intestinal parasites (47.1%), followed by the day care group (35.4%), children …

    brazil-ufba Repository record

  15. Single Nucleotide Polymorphisms of the ATP1a2 gene and Their Effects on Blood Pressure and Other Cardiovascular Variable in African Americans and Caucasian Americans

    Thakre, Tushar P.2007

    Thakre, Tushar P., Single Nucleotide Polymorphisms of the ATP1a2 Gene and Their Effects on Blood Pressure and Other Cardiovascular Variables in African Americans and Caucasian Americans. Doctor of Philosophy (Integrative Physiology), May 2007. Mutations in the 3’ – untranslated region (3’-UTR) of …

    tdl Repository record

  16. Insertion sequence IS1141: discovery, characterization, and association with Mycobacterium intracellulare colonial variation

    Via, Laura Ellen AkersVirginia Tech1993

    … The first objective was to clone the unique DNA fragment present in the 68 kb plasmid but absent from the 62 kb plasmid. The second and third objectives were to determine if the unique fragment contained a transposable element and to analyze the role of that element in the mechanism of colonial …

    vt Repository record

  17. Human immunodeficiency virus type-1 distribution in South Africa and the relevance of genetic diversity on vaccine design

    Van Harmelen, Joanne HeidiDivision of Medical Microbiology1999

    … of HIV-1 gag subtypes was developed based on restriction fragment length polymorphism (RFLP) analysis of 400bp (p17) or 650bp (p17 and 5' p24) long PCR fragments. This strategy was appl i ed to eighty-six samples (Cape Town n=47, Johannesburg n=20, Bloemfontein n=17 and Durban n=2) previously …

    cape-town Repository record

  18. The molecular genetics of Type 2 diabetes

    Saker, Philip JohnThe Open University1995

    … chain reaction (PCR), microsatellite markers, restriction fragment length polymorph isms (RFLPs), mutation screening, separation of PCR products and direct sequencing of them. <br></br><br></br> Chapter 3 investigates the role of the glucokinase gene in five pedigrees with maturity onset …

    the-open-u Repository record

  19. An Investigation for Genetic Susceptibility to Type 1 Diabetes Mellitus and its Microvascular Complications

    Hegazy, D.M.2004

    … reaction (PCR)-based microsatellite analysis and restriction fragment length polymorphism (RFLP) analysis were used to genotype the genes coding for NFκB, HSP70-A2, sorbitol dehydrogenase (SORD) and protein kinase C- β(PKC-β). The A10 allele of the NFκB gene and H3 and H7 alleles of the HSP70-A2 …

    plymouth Repository record

  20. Determining role of bacteria associated with bacterial spot on pumpkin and squash fruit in the North Central Region of the United States

    Liu, QiongUniversity of Illinois at Urbana-Champaign2015

    … Both multilocus sequence typing (MLST) and restriction associated DNA sequence analysis (RADseq) were used to study genetic variation among X. cucurbitae isolates. Twenty X. cucurbitae isolates from Illinois, Indiana, Kansas, Ohio, and Wisconsin were subjected to MLST analysis with three …

    uiuc Repository record

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