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Showing 1 to 20 of 79 for “"Restriction enzyme"”.

  1. Propagation and Restriction Enzyme Mapping of Bacteriophage Pbli Dna

    Entrup, Mark Henry

    … the phage and purified. The DNA was cleaved by restriction endonucleases and the fragments were resolved by agarose gel electrophoresis. The enzymes used and the number of fragments produced by each were; BamHI (4), BglI (6), EcoRV (6), and SstII (4). Through the use of partial, simultaneous, …

    mo-state Repository record

  2. Restriction Enzyme Analysis of Incomplete Parental Replicative Forms of øX174

    Bogardus, Alicia Morales

    … contradict that interpretation.</p> <p>Using restriction enzymes we have re-examined the incomplete RF made from UV-damaged phage. Parental RF made <em>in vivo</em> from viruses exposed to various UV doses were purified by velocity sedimentation and benzoylated naphthoylated …

    loma-linda Repository record

  3. On the Repair of DNA Breaks and the Specificity of the EcoRI Restriction Enzyme

    Heitman, Joseph

    <p>We would like to understand how proteins and enzymes interact with DNA. We describe here our studies of the Mrr methylation dependent restriction system, DNA single- and double-strand break repair in E. coli, and substrate recognition by the EcoRI endonuclease. Many species of bacteria make …

    rockefeller Repository record

  4. Genetic Analysis of Clostridium Acetobutylicum Using Two-Dimensional PFGE to Construct a Macrorestriction Map of ATCC 824 and Restriction Enzyme Profiles for Strain Analysis

    Broussard, Suzanne ReneUniversity of Illinois at Urbana-Champaign2014

    … strains were analyzed using rare-cutting restriction enzymes by one-dimensional and two-dimensional PFGE. C. acetobutylicum strains were placed into three groups based on their restriction enzyme profiles following digestion with SalI, SmaI, and MluI. C. acetobutylicum strains ATCC 824, …

    uiuc Repository record

  5. Identifikasi pola haplotipe DNA mitokondria udang jari (Metapenaeus elegans) Segara Anakan Kabupaten Cilacap Jawa Tengah menggunakan enzim restriksi HindIII

    Nugroho, Fitra Arya DwiUniversitas Islam Negeri Maulana Malik Ibrahim2015

    … haplotype pattern of mitochondrial DNA using a restriction enzyme HindIII. Mitochondrial DNA is only maternal inheritance and has a high variation. Restriction enzyme HindIII used in Klinbunga research (1998) and digested many fragments in penaeidae shrimp. This study aims to find out the number …

    malang Repository record

  6. A molecular biological study on Campylobacter pylori

    Penfold, SonyaDivision of Medical Microbiology1989

    … closed circular DNA was rarely isolated. Restriction enzyme digestion was done with a variety of enzymes, but only 3 of the enzymes used digested the DNA. EcoRI and HindIII partially digested the DNA, while Sau3A digested the plasmids completely, generating 2 fragments of 2,2kb and 2,4kb, …

    cape-town Repository record

  7. Physical mapping of an early sea urchin gene battery from Parenchinus angulosus

    Lawson, T NDepartment of Molecular and Cell Biology1988

    … isolated from Parenchinus angulosus, appeared by restriction enzyme mapping and partial sequencing to be identical to H22, an early histone gene battery isolated from Psammechinus miliaris. (This latter gene was obtained from M. Birnstiel.) This was further confirmed by electron microscopy, and …

    cape-town Repository record

  8. Cassette Systems for Creating Intergeneric Hybrid ATCases

    Simpson, Luci N.University of North Texas1999

    … hybrid ATCases were constructed. An MluI restriction enzyme site was introduced at the carbamoylphosphate binding site within the pyrB genes of both Pseudomonas putida and Escherichia coli. Two hybrids, E. coli pyrB polar domain fused with P. putida pyrB equatorial domain and P. putida …

    unt Repository record

  9. Assessment of Parasitism of House Fly and Stable Fly Pupae (Diptera: Muscidae) by Pteromalid (Hymenoptera: Pteromalidae) Parasitoids Using Polymerase Chain Reaction (Pcr)

    Ratcliffe, Susan TwoheyUniversity of Illinois at Urbana-Champaign2015

    … bands prior to digestion of the PCR product by a restriction enzyme. The sizes of the bands corresponded to the sizes produced by amplification of the DNA of Muscidifurax and Spalangia species, possibly indicating hyperparasitism. Comparison of species composition in paired samples indicated …

    uiuc Repository record

  10. ΑΝΤΙΓΟΝΙΚΗ ΜΕΛΕΤΗ. ΤΥΠΟΠΟΙΗΣΗ ΚΑΙ ΑΝΟΣΟΕΝΖΥΜΑΤΙΚΟΣ ΕΛΕΓΧΟΣ ΕΙΔΙΚΩΝ ΑΝΤΙΣΩΜΑΤΩΝ ΕΝΑΝΤΙ ΤΩΝ ΤΥΠΩΝ 1 ΚΑΙ 2 ΤΟΥ ΙΟΥ ΤΟΥ ΑΠΛΟΥ ΕΡΠΗΤΑ (HSV)

    Markoulatos, Panagiotis, Μαρκουλάτος, ΠαναγιώτηςUniversity of Ioannina1989

    … MONOSPECIFIC SERA. THE RESULTS WERE CONFIRMED BY RESTRICTION ENZYME DNA ANALYSIS IN 29 CASES PROVING THAT THE MONOSPECIFIC ANTISERARAISED AGAINST THE TYPE SPECIFIC ANTIGENS OF EACH TYPE (HSV-1 OR HSV-2) CAN BEUSED IN ELISA FOR THE TYPING OF HSV ISOLATES. PRESENTATION OF RESULTS OF ANTIBODY …

    greece Repository record

  11. Development of High Pressure Surface Plasmon Resonance for the Study of Biosystems

    Kelley, Jennifer GannUniversity of Illinois at Urbana-Champaign2015

    … nuclease, and binding of the BamH1 restriction enzyme to its DNA recognition site.

    uiuc Repository record

  12. Accurate Estimation of Isoform and Gene Expression Levels from Next Generation Sequencing Data

    Nicolae, Marius2011

    … oligo(dT) beads, cDNA cleavage with an anchoring restriction enzyme, and release of cDNA tags using a tagging restriction enzyme whose recognition site is ligated upstream of the recognition site of the anchoring enzyme.</p> <p>In this thesis we present two novel expectation-maximization …

    uconn-diss Repository record

  13. A study of protein-DNA interactions using atomic force microscopy and DNA origami

    Liu, DandanUniversity of Cambridge2019

    … such as viruses invading a bacterium. Restriction enzymes defend the host bacteria by recognising specific sites on the viral DNA and cutting invading viruses at those sites. We aim first to understand how these proteins translocate along the viral DNA molecules toward their recognition …

    cambridge Repository record

  14. Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans

    Emanuel, MargotDivision of Medical Microbiology1990

    … from A. actinomycetemcomitans, digested with a restriction enzyme, Sau3A, ligated to plasmid DNA (pUC18) and transformed into JM109 cells to give a partial A. actinomycetemcomitans library. The library was screened using Southern blot analysis. Out of the nine inserts tested, one was found to be …

    cape-town Repository record

  15. Detection of the calreticulin type 1 and type 2 mutations in a group of myeloproliferative neoplasm patients using molecular methods

    University of Pretoria2016

    … evaluate the use of conventional PCR followed by restriction enzyme digestion, confirmation of the PCR results by nucleotide sequencing, as well as real-time PCR (qPCR) to detect these mutations. A conventional PCR commercial kit was also compared to that of the developed conventional PCR. For …

    pretoria Repository record

  16. Characterizing potential secretion components that increase secretion of recombinant proteins in Pichia Pastoris

    Bulahan, Rhobe Justine Artates2012

    … mutant strains were developed by the method of restriction enzyme mediated integration. These mutants have the ability to secrete β-galactosidase at higher levels in comparison to the wild type. This study focused on characterizing the specific mutant ah2 for its ability to secrete HRP, SLPI, …

    u-pacific Repository record

  17. Passive micromixers for DNA analysis using CFD modelling

    Agarwal, RiteshEast Carolina University2018

    … between Deoxyribonucleic acid (DNA) sample and restriction enzyme for a fast digestion process. Mixing is improved by chaotic advection through serpentine mixing channels. This leads to the desired reduction in the fluid diffusion path while at the same time increasing the fluid contact areas. …

    ecu Repository record

  18. The association of bovine parvovirus DNA and proteins with the nuclear matrix of infected cells

    Briggs, Laura LeeVirginia Polytechnic Institute and State University1983

    … with a subsequent rise to 13% at sixteen hours. Restriction enzyme analysis of the matrix DNA indicated that no specific portion of the BPV genome was responsible for its attachment to the matrix at the selected times. In addition both the nonstructural BPV protein, NP-1, and the capsid proteins …

    vt Repository record

  19. Characterization of components that increase secretion of recombinant proteins in pichia pastoris

    Larsen, Sasha Ellen Marie2011

    … lab previously developed mutant strains using restriction enzyme-mediated integration that enabled P pastoris to secrete Pgalactosidase at higher levels than the wild type strain. This study focuses on characterizing the random pREMI-Z mutations in the genomic DNA and examining their secretory …

    u-pacific Repository record

  20. Characterizing phenotypes of Pichia pastoris mutants that show enhanced secretion of recombinant proteins

    Weaver, Jun Eon2018

    … mutant strains of Pichia pastoris using the restriction enzyme mediated integration method. The mutants exhibited an unusual ability to supersecrete beta-galactosidase, due to the effects of a randomly disrupted gene by pREMI-Z. To learn more about the novel effects of the gene disruption, …

    u-pacific Repository record

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