Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Restriction Site-associated Dna Sequencing"”.
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Linkage mapping in tetraploid blackberry (Rubus spp.) using high-throughput genomic sequencing and restriction-site associated DNA sequencing (RAD-seq)
… markers. More recently, high-throughput genomic sequencing has enabled the discovery of an abundance of co-dominant, single nucleotide polymorphic (SNP) markers, at orders of magnitude greater than previous marker discovery methods. The purposes of this research are 1) to derive a linkage map of …
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Evolutionary and ecological genomics in deep-sea organisms
… characterize eukaryotic genome diversity using restriction-site associated DNA sequencing. These tools are then applied to test global- and regional-scale biogeographic hypotheses of vent fauna using barnacles as model. I suggest that the geological processes and dispersal mechanisms discussed …
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Genetic Diversity of Blue Wildebeest (Connochaetes taurinus) in the Liuwa Plain of Zambia
<p>Advances in DNA sequencing are enabling researchers to identify and genotype large sets of single nucleotide polymorphisms (SNPs) in non-model organisms to answer questions concerning ecology, evolution, and conservation. Analysis of SNP allele frequencies can provide insight into the amount of …
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Systematics and Conservation of the Genus <em>Malacothamnus </em>(Malvaceae)
… to be tested in a phylogenetic framework using a restriction site-associated DNA sequencing (RADseq) dataset. Samples were analyzed using maximum likelihood analysis, quartets-based species tree analysis, neighbor-net analysis, and multidimensional scaling. Based on the totality of evidence, a new …
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Population Structure of Lethrinus Lentjan (Lethrinidae, Percoidei) Across the South China Sea and the Philippines Is Detected With Lane-Affected RADSeq Data
… central and southern Vietnam. This study used restriction-site associated DNA sequencing (RADSeq) to sample single nucleotide polymorphism markers from throughout the genome. Here, RADseq data is successfully used to detect structuring within the Philippines where a previous study of <em> L. …
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The drivers of variation in susceptibility to the amphibian-killing fungus, batrachochytrium dendrobatidis
… or heterozygosity at microsatellite loci. Restriction site-associated DNA sequencing (RADseq) was then used to genotype individuals exposed to Bd at over 10,000 single nucleotide polymorphism (SNP) loci. This revealed clear genetic structure not discernible through microsatellite analysis. …
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Hybridization And Introgression In Marine Invertebrates: Population Genomics Of Phymactis Papillosa And Asterias Sea Stars
… characterized by contrasting genomic datasets: restriction-site associated DNA sequencing (RADseq) SNP data for the eastern Pacific sea anemone <em>Phymactis</em> <em>papillosa</em> and whole genome sequencing (WGS) data for the North Atlantic sea stars <em>Asterias rubens</em> and <em>Asterias …
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Evolutionary and ecological genomics in deep-sea organisms
… characterize eukaryotic genome diversity using restriction-site associated DNA sequencing. Using these tools I find that the predictability of restriction site frequencies in eukaryotic genomes is chiefly determined by the phylogenetic position of the target species and the recognition sequence …
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The Convoluted Origin and Diversification of Strangler Figs in the Neotropics
… fig, F. velutina (sect. Americanae). I combined DNA reads from three sequencing platforms (PacBio Sequel II, Nanopore MinIon, and Illumina Miseq). With this data I was able to assemble 92% of the F. velutina genome. 56.4% of the genome was annotated with either gene predictions or repetitive …
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Population Genetic Structure of Ixodes pacificus Ticks and Detection of Their Pathogens In-Silico
Ticks are hematophagous ectoparasites responsible for the transmission of several serious illnesses in humans, domestic animals, livestock, and wildlife. As climate changes and ticks are introduced into new areas by their host, regions unfamiliar with tick-borne disease become vulnerable to …
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Population Sequencing for Studying Natural and Artifcial Variation in C. elegans
<p>The advent of high coverage and low cost sequencing technologies has allowed for</p><p>newer and more powerful approaches in molecular and population genetics. Transposon</p><p>sequencing, where genome-saturated mutant populations allele frequencies are</p><p>measured before and after selection, …
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Understanding gender determination in dioecious Amaranthus weeds palmer amaranth (Amaranthus palmeri) and waterhemp (Amaranthus tuberculatus)
… both these weedy species. In this work, we use restriction-site associated DNA sequencing (RAD- Seq) to investigate the genetic basis of sex determination in A. tuberculatus and A. palmeri. For each species, approximately 200 plants of each sex were sampled and used to create RAD-Seq libraries. …
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Sex chromosome evolution of papaya: Dynamic structural and expression changes and identification of associated traits
… that control sex type, but they also have genes associated with the different sexes. While the vegetative forms of the three sexes are phenotypically identical, the reproductive structures are unique. In stark contrast to female and hermaphrodite flowers on male plants are borne on long pendulous …
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Behavioral and genomic patterns of evolution during speciation via reproductive and agonistic character displacement
… in genetic incompatibilities. Analyses of restriction-site associated DNA sequencing (RADseq) data in laboratory-generated backcross hybrids revealed strong selection against recombinant individuals. This represents one of the few studies to use fine-scale ancestry mapping in hybrids to …
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Causes and consequences of genetic diversity in pinnipeds
… elephant seal. Using a novel genome sequence and restriction-site associated DNA sequencing, we infer both an extreme recent bottleneck and a likely post-glacial expansion of the species. In chapter 4, we characterise the chemical basis of olfactory mother-offspring recognition in Antarctic fur …
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The ballad of banded killifish: Examining the invasion of a native transplant fish in the Great Lakes region
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
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Dissecting key biomass and adaptation traits of Miscanthus through genetic mapping, exploring mechanisms of cold tolerance, and flowering time in Miscanthus and Saccharum
… nucleotide polymorphism markers generated from restriction site-associated DNA sequencing. We confirmed for the first time a whole-genome duplication in M. sacchariflorus relative to Sorghum bicolor, similar to that observed previously for M. sinensis. Four quantitative trait locus (QTL) …