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Showing 1 to 4 of 4 for “"Restriction Endonuclease Protection Selection and Amplification (REPSA)"”.
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Intrinsic Cleavage Inhibition by Thermophilic Type II-S Restriction Endonucleases
<p>Restriction endonucleases (REs) are the cornerstone research tools used in molecular biology and biotechnology. These enzymes are essential to several ground-breaking achievements, including DNA cloning, gene editing, and genome mapping. A subset of these enzymes, Type II-S restriction …
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Determination and Dissection of DNA-binding Specificity for the Thermus thermophilus HB8 Transcriptional Regulator TTHB099
… HB8. Using the <em>in vitro</em> iterative selection method Restriction Endonuclease Protection, Selection and Amplification (REPSA), we identified the preferred DNA-binding motif for TTHB099, 5'-TGT(A/g)n(t/c)c(t/c)(a/g)g(a/g)n(T/c)ACA-3', and mapped potential binding sites, and regulated …
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Explorations of Thermus thermophilus HB8 Transcription Factors TTHA1437 and TTHA1719 using the Combinatorial Selection Method REPSA
… is known regarding the genes they regulate and their biological functions. The novel combinatorial method Restriction Endonuclease Protection, Selection, and Amplification (REPSA) has been successful for identifying and validating consensus binding sequences in <em>T. thermophilus </em>HB8 …
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Discovery of a DNA-binding Consensus and Potential Genomic Regulatory Binding Sites for the Thermus thermophilus HB8 Transcriptional Regulator TTHA1359
… knowledge of the DNA-binding specificities and genes regulated by many TFs, including those of well-studied model organisms such as <em>Escherichia coli</em> and <em>Thermus thermophilus,</em> remains incomplete or lacking which renders gaps in the understanding of the regulatory networks …